Genetics Chapter 20

How do you analyze a site-directed mutation?

By introducing it into a living organism

DNA sequencing is used to determine the order of ____________ ____________ in a gene

DNA nucleotides

How is ethidium bromide used to analyze amplification by PCR?

Ehtidium bromide stains DNA, allowing the size of a fragment in a gel to be determined

_________-____________ _____________ allows a researcher to produce a mutation at a specific sequence

Site-directed mutagenesis

When using PCR to amplify DNA, short oligonucleotides called primers

flank the region of DNA to be amplified

In 1958 Kary Mullis developed a way to copy DNA without vectors or host cells called

polymerase chain reaction

During PCR, the process of _____________ _______________ results when the Taq polymerase catalyzes the synthesis of complementary DNA, starting at the primers

primer extension

The enzyme ____________ __________________ is used when PCR is employed to detect and quantify the amount of a specific RNA

reverse transcriptase

The dideoxy sequencing method results in mixtures of DNA strands of different lengths which are run on a gel. The gel is read from the bottom up. This is referred to as a ____________ ______________

sequencing ladder