Genetics Chapter 20
How do you analyze a site-directed mutation?
By introducing it into a living organism
DNA sequencing is used to determine the order of ____________ ____________ in a gene
DNA nucleotides
How is ethidium bromide used to analyze amplification by PCR?
Ehtidium bromide stains DNA, allowing the size of a fragment in a gel to be determined
_________-____________ _____________ allows a researcher to produce a mutation at a specific sequence
Site-directed mutagenesis
When using PCR to amplify DNA, short oligonucleotides called primers
flank the region of DNA to be amplified
In 1958 Kary Mullis developed a way to copy DNA without vectors or host cells called
polymerase chain reaction
During PCR, the process of _____________ _______________ results when the Taq polymerase catalyzes the synthesis of complementary DNA, starting at the primers
primer extension
The enzyme ____________ __________________ is used when PCR is employed to detect and quantify the amount of a specific RNA
reverse transcriptase
The dideoxy sequencing method results in mixtures of DNA strands of different lengths which are run on a gel. The gel is read from the bottom up. This is referred to as a ____________ ______________
sequencing ladder