Media Lab- Vitamin D

What are the potential interferences in a patient sample that, if present, may cause erroneous results in immunoassays for vitamin D?

All of the above.

Rickets affects what population?

Children

A 40 year old woman from Alaska presents to her physician with muscle aches and pains and generalized weakness. The following results were obtained. What is most likely the cause of her symptoms?

Decreased Vitamin D.

June is a 63 year-old female that takes a multivitamin daily that contains 400 IU of vitamin D and takes in another 50 IU from her diet. June feels she is getting enough vitamin D but her family is concerned that she is not getting the recommended daily allowance. Do you agree with June or her family?

Her family is correct. June is not getting the recommended daily allowance of Vitamin D from the supplement alone,

John is a 56 year old veterinarian who was recently tested for Vitamin D levels. His current result came back inconsistent with his results in the past. What could be the explanation for this erroneous result?

Heterophile antibodies are present,

A physician calls the laboratory to add on a Vitamin D test to a sample drawn 2 days ago. The laboratory assistant pulls the sample from the refrigerator, generates a test order and label, and delivers the sample to the Chemistry department. The technologist performing the test is using a spectrophotometer-type instrument and notices the sample is grossly hemolyzed. Should the tech continue to run the test?

No- The sample is grossly hemolyzed and this may interfere with the testing methodology.

The sample seen in the image was obtained on a patient from the ICU at 5:00 am this morning. The following tests were ordered on the sample: Electrolytes Vitamin D (using a spectrophotometric method). Will you accept the sample to run both tests?

Reject the sample for both tests.

All of the following are functions of vitamin D, EXCEPT?

Stimulates maturation of fat cells.

Which of the following types of samples are NOT appropriate for vitamin D testing?

Urine and throat swab.

Sarah is a 75 year old retired school teacher originally from Minnesota. She now lives in Florida and frequents the beach 4 days a week. Which form of Vitamin D would she most likely test the highest for?

Vitamin D3