Microbiology lab on Stain
Why are endospores difficult to stain?
due to the high water content within the endospore
Which of the following would be the least likely to stimulate endospore formation?
radiation
What color are acid fast organisms?
red vegetative cells
What added ability would a microbial cell have if it possesses a capsule?
All of the above are added abilities
This term is defined as one flagella located at each end of the cell
Amphitrichous
This term is defined as tuft of flagella emanating from one position at one end of the cell.
Lophotrichous
The term is defined as a single flagellum located at the end.
Monotrichous
What added ability would a microbial cell have if it possess flagella?
Motility
Acid-fast staining is clinically important for which genus?
Mycobacterium and Nocardia
The term is defined as many flagella around the entire surface of the cell.
Peritrichous
Which of the following diseases are commonly known to produce endospores?
Bacillus anthracis (Anthrax)
What disease state might the acid fast stain be useful for aiding in identification?
Tuberculosis
The present of what material requires the usage of a strong decolorizer in the Ziehl-Nielsen acid fast staining procedure?
Wax D
Which type of specimen would be the best for the observation of an endospore within a vegetative cell?
a 3-5 day old broth or agar culture
This protects some bacterial cells against ingestion by phagocytosis of a host leukocyte or by protozoans.
capsule
This is the primary stain used in the acid-fast staining procedure.
carbol fuchsin
Why is acid alcohol used to decolorize the bacteria in the acid fast stain?
Acid Fast bacteria are resistant to decolorization even when acid is added to the alcohol
What type of fixation procedure should be used for the Anthony's Capsular staining procedure?
Alcohol fixation
Which of the following is not true regarding endospore production?
Endospores production is a method of cellular reproduction.
Why are acid fast organisms difficult to stain?
The cell contain a lot of wax within the cell construction
What added ability would a microbial cell have if it forms endospore?
enhanced survival in harsh environments
This is used to induce the dye to penetrate and combine strongly with the bacterial cell and thus resist decolorization during the acid-fast staining procedure.
heating the stain on the slide until it steams
When does sporulation begin in endospore forming microorganisms?
in a culture that is 2-3 days old
The stain used in the Negative staining method of capsules
india ink or nigrosin
What characteristics of endospores allow their extreme resistance to heat and chemicals?
low water content and the presence of calcium dipicolinate
The primary stain used in the endospore staining procedure was called
malachite green
This is the counter-stain used in the acid-fast staining procedure.
methylene blue
