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When changing magnification from 10x to 40x, the specimen disappears
Adjust the iris diaphragm to let in more light
Your field of view is partially obscured by dark area
Check to make sure the objective lens is clicked into position or check to see if the objective and ocular lenses are clean
There are hollow, dark circles in your field of view
These are air bubbles. Lift the cover slip and lower it gently at 45 angle to expel the air
There's a fingerprint in your field of view
Use lens paper to clean the slide, the objective lenses, and the ocular lens
