Parkinson's Disease Lab

Which parameter increases the resolution in size exclusion chromatography?

A moderate flow rate

How does EGCG prevent aSN toxicity?

By binding to the oligomers, it blocks membrane permeabilization

Which sample fraction do you want to collect?

Fraction eluted at 775 mL

When would you use a vesicle model for studying cell degeneration?

In experiments where the membrane integrity needs to be tested

Which technique would you use to separate proteins by charge?

Ion exchange chromatography

What is the purpose of sonication?

Lysing the cells to release their contents

What is the effect of aSN oligomerization on LUVs?

Membrane permeabilization

Taking into account what you have seen in the observed samples, what would you expect to happen in the sample with EGCG in regards to ASN oligomer toxicity?

No popping of vesicles

Compared to the negative control, what is the effect of aSN monomers on LUVs?

None, they remain the same

Which symptom is not common in Parkinson's disease?

Recurrent bleeding

Which part of the chromatogram represents the aSN oligomers?

The one eluted at 240-280 mL

What is the effect of aSN oligomers on the vesicles?

They pop them

Why do you need a negative control in your experiments?

To measure how the assay works under conditions where you should not get a positive outcome

Why is it important to select a good stationary phase?

To retain the desired molecules and separate them from the rest of the sample.

Hypothesis:

aSN oligomers elute earlier than monomers when performing size exclusion chromatography