Parkinson's Disease Lab
Which parameter increases the resolution in size exclusion chromatography?
A moderate flow rate
How does EGCG prevent aSN toxicity?
By binding to the oligomers, it blocks membrane permeabilization
Which sample fraction do you want to collect?
Fraction eluted at 775 mL
When would you use a vesicle model for studying cell degeneration?
In experiments where the membrane integrity needs to be tested
Which technique would you use to separate proteins by charge?
Ion exchange chromatography
What is the purpose of sonication?
Lysing the cells to release their contents
What is the effect of aSN oligomerization on LUVs?
Membrane permeabilization
Taking into account what you have seen in the observed samples, what would you expect to happen in the sample with EGCG in regards to ASN oligomer toxicity?
No popping of vesicles
Compared to the negative control, what is the effect of aSN monomers on LUVs?
None, they remain the same
Which symptom is not common in Parkinson's disease?
Recurrent bleeding
Which part of the chromatogram represents the aSN oligomers?
The one eluted at 240-280 mL
What is the effect of aSN oligomers on the vesicles?
They pop them
Why do you need a negative control in your experiments?
To measure how the assay works under conditions where you should not get a positive outcome
Why is it important to select a good stationary phase?
To retain the desired molecules and separate them from the rest of the sample.
Hypothesis:
aSN oligomers elute earlier than monomers when performing size exclusion chromatography
