post course bio lab quiz

200x

Compound microscopes have two lenses. If you are looking through one that has a 10x lens and a 20x lens in the viewing path from the specimen to your eye, what is the microscope's total magnification?

0.5 um and 1000 um

Micropipettes are a critical tool when doing molecular research. What is the minimum and maximum amount of liquid a normal set of micropipette can move at a time?

stomata

a plant leaf has a lot of ___ to allow gas exchange

finding the absorbance at 260 nm

a spectrophotometer can be used to quantify DNA by __

error associated with that mean

bars located on a graph of the mean data indicate...

they have evolved to work in a specific environment

enzymes have optical conditions under which they work because ____

by measuring the rate of oxygen produced

how can the rate of photosynthesis of a plant be directly measured?

less

osmosis is the movement of free water from the side of a semi permeable membrane with __ solutes than the other

transformation

placing bacteria in a hot environment for a short period of time while they are surrounded by plasmid DNA is a step in which bio technique?

ligase

the enzyme that covalently links the backbone of one DNA fragment to another DNA fragment is ___

gel electrophoresis

what bio technique is used to separate fragments of DNA by size?

transfer of genes between species

what does the universal nature of the genetic code allow?

restriction enzyme

what enzyme does a scientist use when they want to cut DNA at a specific sequence

it binds double stranded nucleic acids and compliments single stranded DNA moving in the 3' to 5' direction

what role does the DNA polymerase enzyme perform in the PCR reactions?

ATP + NADPH + CO2 --> C6H12O6 + ADP + NADP+

what's the equation of photosynthetic dark reactions?

recombinant DNA

when DNA from two different species are mixed, this is called ____

270,000 nm

when looking at a leaf under a microscope, you notice that a tear in the leaf is 270 micrometers (um) long, what is this value if you convert it to nano meters?

decrease

when using a light microscope, as the magnification increases, we can expect the depth of field to ____

the amount of light that was scattered by your sample

when using a spectrophotometer the output is usually an ABS value, what is this actually measuring?

hypertonic

when you drink a solution that is really salty, your cheek cells are put in an ___ environment

40 times

when you look at a red blood cell and it is 8 um in diameter under the 4x objective lens of a typical lab microscope, how much is the red blood cell being magnified

photosynthesis during the day - respiration during the day and night

your typical C3 photosynthesizing plant performed respiration and photosynthesis during which times of the day?

isolating a sequence of DNA and making multiple copies of it

DNA cloning refers to the process of