Quiz-Molecular basis of inheritance

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In trying to determine whether DNA or protein is the genetic material, Hershey and Chase made use of which of the following facts?

DNA contains phosphorus, whereas protein does not.

Suppose you are provided with an actively dividing culture of E. coli bacteria to which radioactive thymine has been added. What would happen if a cell replicates once in the presence of this radioactive base?

DNA in both daughter cells would be radioactive

Which enzyme catalyzes the elongation of a dNA strand in the 5'-> 3' direction?

DNA polymerase III

What is the basis for the difference in how the leading and lagging strands of DNA molecules are synthesized?

DNA polymerase can join new nucleotides only to the 3' end of a growing strand.

Which of the following statements describes chromatin?

Heterochromatin is highly condensed, whereas euchromatin is less compact.

Which of the following statements is true of histones?

Histone H1 is not present in the nucleosome bead; instead, it draws the nucleosomes together.

Why do histones bind tightly to DNA?

Histones are positively charged, and DNA is negatively charged

Which of the following statements describes the eukaryotic chromosome?

It consists of a single line molecule of double-stranded DNA plus proteins.

What is meant by the description "antiparallel" regarding the strands that make up DNA?

The 5' to 3' direction of one strand runs counter to the 5' to 3' direction of the other strand

To repair a thymine dimer by nucleotide excision repair, in which order do the necessary enzymes act?

endonuclease, DNA polymerase I, DNA ligase

What is the function of topoisomerase?

relieving strain in the DNA ahead of the replication fork

Which of the following help(s) to hold the DNA strands apart while they are being replicated?

single-strand binding proteins

In his work with pneumonia-causing bacteria and mice, Griffith found that

some substance from pathogenic cells was transferred to nonpathogenic cells, making them pathogenic.

What is the function of DNA polymerases III?

to add nucleotides to the 3' end of a growing DNA strand


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