Restriction Enzymes

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What does it mean if a restriction enzymes if class II?

it was discovered second

What are the 2 types of fragment ends?

1. Sticky 2. Blunt

How long are restriction sites?

4-6 base pairs long

What bonds do class II restriction enzymes ALWAYS break?

Covalent Bonds

How do you attach restriction fragments to each other?

DNA Ligase

What is the word used for when a retriction enzymes cuts DNA?

Digest

What bonds do class II restriction enzymes SOMETIMES break?

Hydrogen bonds

Where do restriction enzymes cut?

In the middle of DNA molecules in a specific site

What does it mean if the enzymes if 'endonuclease'?

It cuts in the inside in the middle

What does it mean if an enzymes of "exonuclease'?

It cuts outside by removing the ntds from the temomeres

What are restriction enzymes like?

Molecular Scissors

What are Restriction enzymes used for in daily life?

Recombinant DNA (humulin) DNA Profiling (murder/rape, paternity, Identify Remains)

What is another name for restriction enzymes?

Restriction Endonucleases

What is unique about restriction sites?

They are palindromes

What bonds can sticky ends form and with what?

They can form hydrogen bonds with other complementary fragments

What is unique about restriction enzymes?

They have an evolutionary defense against bacteriophages

What produced restriction enzymes?

Bacterial Cells

What bonds do restriction enzymes break?

Breaks phosphodiester bonds and hydrogen bonds

What does it mean if the fragment is sticky?

The enzyme breaks phosphodiester and hydrogen bonds

What does it mean if the fragment is blunt?

The enzyme only breaks only phosphodiester bonds

What is the cutting criteria of class II restriction enzymes?

Unmethylated Double Stranded DNA

What are bacteriophages?

Viruses that attack bacterial cells


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