The Ames test
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Allows for
easy observation and monitoring of gene expression and mutation rate
mutation of salmonella typhimurium
reversion to his+, produces histidine, grows on plate (back mutation)
salmonella typhimurium
strain with defective histidine gene (his-) point mutation, 1 incorrect base can't grow on medium lacking histidine
the ames test
test to detect chemicals with carcinogenic potential
the ames test uses bacteria
to test the mutagenic effect of certain products
chemicals capable of mutating bacterial DNA
will also likely mutate mammalian DNA
