The Ames test

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Allows for

easy observation and monitoring of gene expression and mutation rate

mutation of salmonella typhimurium

reversion to his+, produces histidine, grows on plate (back mutation)

salmonella typhimurium

strain with defective histidine gene (his-) point mutation, 1 incorrect base can't grow on medium lacking histidine

the ames test

test to detect chemicals with carcinogenic potential

the ames test uses bacteria

to test the mutagenic effect of certain products

chemicals capable of mutating bacterial DNA

will also likely mutate mammalian DNA


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