ap biology - chapter 3

Which of the following statements best describes how a growth factor stimulates cell division from outside a cell?

The growth factor binds to receptors on the cell surface, initiating a signal transduction pathway that activates specific target genes.

When the concentration of GAl is highest, the average height of the control seedlings is approximately

10 cm

In mammals, an increase in the concentration of sodium in the blood triggers the release of antidiuretic hormone (ADH) from the pituitary gland. As the concentration of sodium in the blood returns to previous levels, the release of ADH from the pituitary gland is reduced. Based on the information presented, which of the following describes the most likely role of ADH in maintaining blood osmolarity?

ADH promotes an increase in the movement of water into the bloodstream.

Steroid hormones, such as testosterone, pass through the plasma membrane and bind to an intracellular protein, as shown in the diagram below. The hormone-receptor complex then enters the nucleus, where it interacts with DNA to promote transcription of a specific gene. Based on the information presented, which of the following will also occur in response to steroid signaling?

Production of a specific mRNA will increase as a result of the binding of the hormone-receptor complex to the DNA

Cell communication is critical for the function of both unicellular and multicellular eukaryotes. Which of the following is likely true of cell signaling?

Similar cell signaling pathways in diverse eukaryotes are evidence of conserved evolutionary processes.

The diagram above illustrates feedback control as exerted by the hormone thyroxine. Following surgical removal of the thyroid gland, the level of TSH in the blood will increase. Which of the following best explains this increase?

A decrease in thyroxine levels means a loss of inhibition to the hypothalamus and anterior pituitary, leading to increased TSH secretion.

The graph above shows changes in glucagon and insulin secretions at different concentrations of blood glucose. Which of the following feedback mechanisms is best supported by the data?

A low glucose level causes the release of glucagon, which stimulates the release of more glucose from tissues, which in turn lowers the amount of glucagon being released.

The mechanism of action of many common medications involves interfering with the normal pathways that cells use to respond to hormone signals. Which of the following best describes a drug interaction that directly interferes with a signal transduction pathway?

A medication enters the target cell and inhibits an enzyme that normally synthesizes a second messenger.

The diagram above represents a model of signal transduction pathways (I and II) in a cell that is targeted by two different hormones (H1 and H2). The components of the signal transduction pathways are identified in the figure legend. Each cellular molecule in both pathways can exist in an inactive or active form. When the components in pathway I are sequentially activated, the TAP molecules promote cell division. When the components in pathway II are sequentially activated, downstream signaling by the G protein is inhibited. Based on the model, which of the following mutations is most likely to result in a cell that will generate a cancerous tumor?

A mutation in the gene encoding G-PIP that results in a nonfunctional protein

The figure above shows a model of a ligand precursor being cleaved to produce an active ligand that binds to a specific receptor. Which of the following is most likely to reduce the binding of the active ligand to its receptor?

A mutation in the receptor gene that causes a substitution of a charged amino acid for a nonpolar amino acid in the ligand binding site of the receptor

Damaged tissue releases chemicals that activate platelets and stimulate the formation of blood clots. Which of the following predictions about the activity of platelets best describes a positive feedback mechanism?

Activated platelets release chemicals that activate more platelets.

Based on the model of ferritin synthesis presented in Figure 2, which of the following describes the role of feedback on the control of intracellular iron levels?

An increase in iron levels activates synthesis of ferritin protein. Ferritin protein in turn binds iron, thereby decreasing both free iron levels and ferritin synthesis.

The vertebrate forelimb initially develops in the embryo as a solid mass of tissue. As development progresses, the solid mass near the end of the forelimb is remodeled into individual digits. Which of the following best explains the role of apoptosis in remodeling of the forelimb?

Apoptosis involves the regulated activation of proteins in specific cells of the developing forelimb that leads to the death of those cells.

Antidiuretic hormone (ADH) is important in maintaining homeostasis in mammals. ADH is released from the hypothalamus in response to high tissue osmolarity. In response to ADH, the collecting duct and distal tubule in the kidney become more permeable to water, which increases water reabsorption into the capillaries. The amount of hormone released is controlled by a negative feedback loop. Based on the model presented, which of the following statements expresses the proper relationship between osmolarity, ADH release, and urine production?

As tissue osmolarity rises, more ADH is released, causing less water to be excreted as urine.

The model shown in the figure represents the role of two hormones, calcitonin and parathyroid hormone (PTH), in maintaining normal blood calcium levels in humans. If a dietary change results in an increase in blood calcium concentration above normal levels, which of the following is the most likely effect on calcium homeostasis?

Calcitonin levels will rise, thus promoting the deposit of calcium into bones.

Based on the model, which of the following best explains how regulation of neurotransmitter release might increase the range of responses to a stimulus in the nervous system?

Different neurons in the same neural network can release different amounts of neurotransmitter.

Ethylene gas is an organic molecule that serves many cell signaling roles in flowering plants. Which of the following best explains how a positive feedback mechanism involving ethylene works?

Cells of ripening fruit produce ethylene, which activates the ripening response in other fruit cells.

Previous experiments indicate that CDK5 is active only when attached to a protein called p35. Which of the following best predicts how p35 might play a role in regulating neuron function?

Degradation of p35 results in increased synaptic activity.

Epinephrine is a protein hormone found in many animals. Epinephrine stimulates a signaling pathway that results in the breakdown of glycogen to glucose in the liver cells. Which of the following describes the initial steps in the process whereby epinephrine stimulates glycogen breakdown?

Epinephrine binds to a cell-surface receptor; the activated receptor stimulates production of the second messenger, cAMPM

Which of the following claims is most consistent with the data shown in Figure 2 ?

EspEsp expression is necessary to prevent the overproduction of insulin.

Ethylene is an organic compound produced by ripening fruits. In a controlled experiment, researchers found that ethylene gas stimulated the ripening process in newly harvested fruits. Which of the following describes the most likely connection between natural ethylene production and fruit ripening?

Ethylene gas is a chemical signal through which ripening fruits trigger the ripening process in other fruits.

A researcher claims that the epinephrine signaling pathway controls a catabolic process in muscle cells. Which of the following statements best helps justify the researcher's claim?

Glycogen phosphorylase catalyzes the conversion of glycogen to glucose-1-phosphate.

Based on the data, which of the following best represents how the mice with the implanted hESC-β cells use negative feedback to return blood glucose levels to normal if blood glucose levels increase?

Increased insulin secretion by hESC-β cells → increased glucose uptake by body cells → decreased insulin secretion by hESC-β cells

Many human cells can be stimulated to divide by hormonelike growth factors that bind to receptor proteins (R) on the plasma membrane and trigger an internal signal-transduction cascade. In many cases, however, the process of contact inhibition prevents mitosis when cells are in direct contact with one another. Contact inhibition occurs when proteins called cell adhesion molecules (CAMs) interact, causing them to change shape so that the growth-factor signaling proteins that normally associate with CAMs are replaced by another protein, called M. Both pathways are depicted in the figures below. Which of the following statements accurately uses the information presented to support the hypothesis that interruption of M function in a single body cell can result in cancer?

Growth-factor signaling can trigger mitosis in cells that are in direct contact with other cells

Based on the experimental results, which of the following describes the most likely defect in muscle cells of patients with type 2 diabetes?

IRS-1 activation is reduced at high insulin concentrations.

Anabaena is a simple multicellular photosynthetic cyanobacterium. In the absence of fixed nitrogen, certain newly developing cells along a filament express genes that code for nitrogen-fixing enzymes and become nonphotosynthetic heterocysts. The specialization is advantageous because some nitrogen-fixing enzymes function best in the absence of oxygen. Heterocysts do not carry out photosynthesis but instead provide adjacent cells with fixed nitrogen, in exchange receiving fixed carbon and reduced energy carriers. As shown in the diagram above, when there is low fixed nitrogen in the environment, an increase in the concentration of free calcium ions and 2-oxyglutarate stimulates the expression of genes that produce two transcription factors (NtcA and HetR) that promote the expression of genes responsible for heterocyst development. HetR also causes production of a signal, PatS, that prevents adjacent cells from developing as heterocysts. Based on your understanding of the ways in which signal transmission mediates cell function, which of the following predictions is most consistent with the information given above?

In an environment with low fixed nitrogen, treating the Anabaena cells with a calcium-binding compound should prevent heterocyst differentiation.

The brain coordinates the circulatory and respiratory systems of the human body. The control of breathing, for example, involves neural pathways among the structures represented in the figure above. One important stimulus in the control of breathing is an increase in blood CO2 concentration, which is detected as a decrease in blood pH. Which of the following best describes the physiological response to an overall increase in cellular respiration in the body?

In response to low blood pH, the pH sensors send a signal to the brain, which then sends a signal to the diaphragm, resulting in an increased rate of breathing to help eliminate excess blood CO2

Cortisol is a hormone produced in response to stress, including starvation, in humans. Which of the following is most likely an immediate effect of a starvation-induced increase in cortisol secretion?

Increased mobilization of fatty acids from fat cells

Insulin is a protein hormone that is secreted in response to elevated blood glucose levels. When insulin binds to its receptors on liver cells, the activated receptors stimulate phosphorylation cascades that cause the translocation of glucose transporters to the plasma membrane. Based on the information provided, which of the following best describes the role of insulin in this liver cell signal transduction pathway?

It acts as a ligand

In a certain signal transduction pathway, the binding of an extracellular molecule to a cell-surface protein results in a rapid increase in the concentration of cyclicAMP inside the cell. The cyclic AMP binds to and activates cytosolic enzymes that then activate other enzymes in the cell. Which of the following statements best describes the role of cyclic AMP in the signal transduction pathway?

It acts as a second messenger that helps relay and amplify the signal within the cell.

Ethylene causes fruits to ripen. In a signaling pathway, receptors activate transcription factors, which ultimately leads to ripening. Which of the following best supports the claim that ethylene initiates the signal transduction pathway that leads to ripening of fruit?

Loss-of-function mutations in ethylene receptors result in changes to the ripening process.

Figure 1 is a proposed model of the feedback system controlling erythrocyte (red blood cell) production. Air is less dense at very high elevations, so less oxygen is available than in the denser air at sea level. Based on the model in Figure 1, if a person travels from sea level to a high elevation location, which of the following correctly predicts the response to the decreased blood oxygen level?

More erythropoietin will be secreted from the kidneys, increasing production of erythrocytes.

Which of the following is a correct conclusion that can be drawn from the data in Figures 1, 2, and 3?

Most of the increase in the concentration of all gibberellins 40 to 45 days after planting is due to an increase in the concentration of GAl.

Which of the following was a positive control in the experiment?

Normal strain

Cyclic AMPAMP phosphodiesterase is an enzyme that catalyzes the conversion of cyclic AMPAMP to a different molecule. Which of the following best predicts the effect of inhibiting cyclic AMPAMP phosphodiesterase in a muscle cell stimulated by epinephrine?

Phosphorylase kinase will remain active because protein kinase A will no longer be deactivated.

In flowering plants, plasmodesmata are narrow channels through cell walls that connect the cytoplasms of adjacent cells. An explanation of how plant cells communicate across cell walls will most likely refer to the diffusion through plasmodesmata of which of the following?

Small, water-soluble molecules

A student claims that the YY chromosome contains the sex-determining region gene, known as the SRYSRY gene, which causes male fetuses to develop testes. Which of the following provides correct information about cell signaling that supports the claim?

The SRY gene produces a protein that binds to specific regions of DNA in certain tissues, which affects the development of these tissues.

Type 1 diabetes results from the destruction of insulin-producing cells in the pancreas. Individuals with type 1 diabetes produce insufficient amounts of insulin, a hormone that regulates the concentration of glucose in the blood. Which of the following best explains how treatment with a drug that stimulates the production of insulin receptors on target cells will affect the insulin signaling pathway in an individual with type 1 diabetes?

The drug will have little or no effect on the signaling pathway because the receptors will not be activated in the absence of insulin.

Glucocorticoids are steroid hormones that control cellular responses through several different signaling pathways. One of the signaling pathways involves the glucocorticoid receptor, an intracellular protein that is activated by binding to a glucocorticoid molecule. A simplified model of the glucocorticoid receptor signaling pathway is represented in Figure 1. Which of the following statements best predicts the effect of a mutation that results in a loss of the glucocorticoid receptor's ligand binding function?

The glucocorticoid receptor will remain associated with the accessory proteins.

The following questions refer to the data in Figures 1 through 4 below, which were collected during a study of the growth of plant seedlings. Which of the following is a correct conclusion that can be drawn based only on the data in Figure 4?

The greater the inhibition of GAl synthesis, the lower the plant height after 80 days.

The epinephrine signaling pathway plays a role in regulating glucose homeostasis in muscle cells. The signaling pathway is activated by the binding of epinephrine to the beta-2 adrenergic receptor. A simplified model of the epinephrine signaling pathway is represented in Figure 1. Which of the following outcomes will most likely result from the irreversible binding of GDPGDP to the G protein?

The intracellular concentration of glycogen will increase.

Figure 1 shows a model of a signal transduction cascade, initiated by the binding of a ligand to the transmembrane receptor protein A. A DNA mutation changes the shape of the extracellular domain of transmembrane receptor protein A produced by the cell. Which of the following predictions is the most likely consequence of the mutation?

The molecule that normally binds to protein AA will no longer attach, deactivating the cellular response.

Which of the following best explains how small molecules move between adjacent cells in a plant shoot?

The molecules pass freely through plasmodesmata, which are cytoplasmic strands connecting two cells.

In response to elevated blood glucose levels, beta (β) cells in the pancreas release insulin, a regulatory hormone. Insulin signals body cells to take up glucose from the blood, which returns blood glucose levels back to normal. Type 1 diabetes is an autoimmune disorder that destroys β-cells, resulting in elevated blood glucose levels. Researchers have proposed that diabetes could be treated by implanting human embryonic stem cells (hESCs) that have been induced to develop into β-cells (hESC-β). To test the proposed treatment, the researchers set up two groups of genetically identical mice and implanted the mice from one group with hESC-β cells. Several weeks after the hESC-β implant, both groups of mice were given a drug (STZ) that selectively destroys the naturally occurring mouse β-cells but does not affect the implanted hESC-β cells. Figure 1 shows a comparison of average blood glucose levels in both groups of mice. In a continuation of the experiment, the researchers removed the hESC-β implant from one of the mice 16 weeks after STZ treatment. Figure 2 shows the blood glucose levels in the mouse over the duration of the experiment. Based on the data in Figure 2, which of the following best justifies the researchers' claim that hESC-β implants can functionally replace the mouse's naturally occurring β-cells?

The mouse with an hESC-β implant successfully regulated blood glucose levels after STZ treatment but not after the implant was removed.

Hormones are chemical signals that are released by cells in one part of the body that travel through the bloodstream to signal cells in another part of the body. Insulin is a hormone that is released by the pancreas that induces the uptake of glucose molecules from the bloodstream into cells. In this way, insulin lowers the overall blood glucose levels of the body. Osteoblasts and osteoclasts are two types of bone cells that play a role in regulating blood glucose levels (Figure 1). Binding of insulin to the insulin receptor on osteoblasts activates a signaling pathway that results in osteoblasts releasing a molecule, OPGOPG, that binds to neighboring osteoclasts. In response, the osteoclasts release protons (H+H+) and create an area of lower pHpH outside the cell. This low pHpH activates osteocalcin, a protein secreted in an inactive form by osteoblasts. The EspEsp gene encodes a protein that alters the structure of the insulin receptor on osteoblasts and interferes with the binding of insulin to the receptor. A researcher created a group of osteoblasts with an EspEsp mutation that prevented the production of a functional EspEsp product (mutant). The researcher then exposed the mutant strain and a normal strain that expresses EspEsp to glucose and compared the levels of insulin in the blood near the osteoblasts (Figure 2). Based on the information provided, which of the following best justifies the claim that osteocalcin is a hormone?

The osteoblasts in the bone secrete osteocalcin, which causes cells in the pancreas to change their activity.

The coagulation cascade controls blood clot formation in response to blood vessel injury. Thrombin is an enzyme that plays a key role in regulating the coagulation cascade. A simplified model of thrombin's role in regulating the coagulation cascade is represented in Figure 1. Argatroban is a competitive inhibitor of thrombin. Which of the following effects on the coagulation cascade is most likely to result from inhibiting thrombin activity with argatroban?

The rate of fibrin formation will decrease.

Which of the following best describes the scientists' findings concerning the density of symbionts presented in Figure 2 ?

The symbiont density at 32°C32°C was different from the density at 27°C27°C on days 55 and 1010 of the experiment.

In contrast to type 1 diabetes, where there is no insulin production, type 2 diabetes is characterized by a failure of body cells to respond normally to insulin. Based on the results of the experiment, which of the following best predicts the effectiveness of using hESC-β implantation to treat type 2 diabetes?

The treatment would fail because insulin produced by implanted hESC-β cells will not stimulate glucose uptake by body cells.

Trypsinogen is split by the enzyme enterokinase to form an activated molecule of the protease trypsin. Which of the following would confirm that the activation of trypsin is an example of how a positive feedback mechanism can amplify a biological process?

The trypsin produced by the reaction is capable of splitting and activating additional trypsinogen molecules.

Platelets are fragments of larger cells and normally circulate in the blood without adhering to blood vessel walls. When the wall of a blood vessel is damaged, collagen fibers in the wall are exposed to the interior of the blood vessel. The exposed fibers and chemicals released from the endothelial cells that line the blood vessel attract platelets, which start to form a plug and release other chemicals (Figure 1). Which of the following best explains the feedback mechanism illustrated in Figure 1 ?

This is an example of positive feedback, because the few platelets that initially bind attract more platelets to the damaged area.

Notch is a receptor protein displayed on the surface of certain cells in developing fruit fly embryos. Notch's ligand is a membrane-bound protein called Delta that is displayed on the surface of adjacent cells. When Notch is activated by its ligand, the intracellular tail of the Notch protein becomes separated from the rest of the protein. This allows the intracellular tail to move to the cell's nucleus and alter the expression of specific genes. Which of the following statements best explains Delta's role in regulating cell communication through the Notch signaling pathway?

Delta restricts cell communication to short distances within a developing embryo.

Researchers investigating the regulation of neurotransmitter release from presynaptic neurons proposed a model (Figure 1) in which CDK5, a protein expressed in axon terminals, inhibits the movement of synaptic vesicles to the presynaptic membrane. To test their model, the researchers used a modified version of green fluorescent protein GFP. In slightly alkaline conditions, GFP exhibits a bright green fluorescence. In acidic conditions, GFP exhibits no fluorescence. Using standard techniques, the gene encoding GFP is easily introduced into living cells. By engineering the expression of GFP in laboratory-cultured nerve cells, the researchers found that a bright green fluorescence was exhibited only when a presynaptic neuron was given a certain stimulus. Which of the following observations best supports the hypothesis that CDK5 negatively regulates neurotransmitter release?

Inhibition of CDK5 activity in neurons increases the movement of synaptic vesicles to the plasma membrane in response to a specific stimulus.

A researcher observes that mice from the mutant strain experience low blood sugar. Which of the following best describes the feedback mechanism in the pathway (Figure 1) causing the low blood sugar in the mutant strain?

The positive feedback of insulin production

Precise regulation of specific hormone levels is required for optimal sperm production in mammals, as summarized in the figure above. Anabolic-androgenic steroids (AAS) are synthetic variants of testosterone that are sometimes abused by persons who desire to enhance their athletic performance or alter their physique. Assuming that AAS function in the same way as naturally occurring testosterone, it is most likely that long-term abuse of AAS would

reduce sperm production

Based on the information presented, which of the following genetic changes in an individual without diabetes is most likely to result in a disrupted cellular response to insulin signaling similar to that of an individual with type 2 diabetes?

A deletion in the gene encoding the insulin receptor that removes only the cytoplasmic domain of the protein

Certain reef-building corals contain photosynthetic, symbiotic algae that have the ability to make dimethylsulphoniopropionate (DMSPDMSP), a chemical involved in the marine sulfur cycle. DMSPDMSP is released into the surrounding water, where it is converted to the gas dimethyl sulfide (DMSDMS) by microorganisms and enters the atmosphere. Once in the atmosphere, it triggers the formation of sulfate aerosols, which induce cloud formation and block sunlight from heating up the water. The symbiotic algae produce DMSPDMSP when they are stressed by a high water temperature. If water temperature is too high, corals will expel the symbiotic algae that produce DMSPDMSP. Researchers measured the amount of DMSPDMSP produced by juvenile and adult coral and their symbionts under normal and thermally stressed conditions. The data are shown in the graphs in Figure 1. Figure 1: DMSPDMSP concentration in juvenile and adult corals and their symbionts in normal and thermally-stressed conditions. Error bars represent ±2SEx¯±2SEx¯. The researchers also measured the density of the symbiont as well as the photosynthetic yield in adult corals at the two temperatures. Photosynthetic yield is an index measure of energy output compared to sunlight energy input in which larger photosynthetic yield values represent photosynthetic organisms producing more energy. Figure 2: Variation in symbiont density and photosynthetic yield in adult corals grown in normal and thermally-stressed conditions. Error bars represent ±2SEx¯±2SEx¯. Which of the following best describes the production of DMSPDMSP by coral and coral symbionts?

A negative feedback mechanism that reverses the environmental change

Scientists compared the chemical structure of several molecules that various bacterial species use for quorum sensing. Quorum sensing is an ability some bacteria have to detect the number of related cells nearby. The chemical structure of some of these molecules found in certain species of bacteria are shown in Figure 1. Figure 1. The chemical structure of several molecules used for quorum sensing in three species of bacteria Which of the following research questions would best guide an investigation of the link between the structure of the signaling molecules and the evolution of quorum sensing?

Did these species evolve from a common ancestor that used a similar signaling molecule?

Which of the following best describes the effect of insulin binding to the receptor on the osteoblast cells?

Insulin binding ultimately increases pancreatic secretion of additional insulin.

Blood vessels are surrounded by cells called smooth muscle cells. Nitric oxide triggers a signaling cascade in smooth muscle cells that causes the cells to relax (Figure 1). Which of the following is represented by the gradual increase in thickness of the arrows from the top to the bottom of Figure 1 ?

The number of signaling molecules that are produced or activated increases with each step in the pathway.

Vertebrate immune responses involve communication over short and long distances. Which of the following statements best helps explain how cell surface proteins, such as MHCMHC proteins and T cell receptors, mediate cell communication over short distances?

The proteins interact directly with proteins on the surfaces of other cells.

Insulin, a hormone secreted by pancreatic cells, stimulates glucose uptake in skeletal muscle cells by mobilizing glucose transporter proteins (GLUT4) to the plasma membrane. As depicted in Figure 1, binding of insulin to the insulin receptor triggers an intracellular signaling cascade in which certain molecules activate other molecules in a relay of the hormone signal to cell targets. One outcome of the signaling cascade is mobilization of GLUT4 from vesicle storage sites in the cytoplasm to sites at the cell surface, where GLUT4 allows glucose to enter the cell. In type 2 diabetes, the cellular response to insulin is disrupted, and individuals with type 2 diabetes cannot properly regulate their blood glucose levels. In an investigation of the insulin signaling pathway, samples of skeletal muscle were isolated from individuals who have type 2 diabetes and from individuals who do not. The results of several experiments that were performed on the muscle samples are shown in Figure 2, Figure 3, and Figure 4. Which of the following is a valid interpretation of the experimental results that explains how individuals with type 2 diabetes differ from individuals without diabetes?

The relatively low levels of glucose uptake in individuals with type 2 diabetes indicate that mobilization of GLUT4 to the cell surface is reduced in muscle cells of those individuals.

Excess intracellular iron is toxic to cells (iron-induced toxicity). Ferritin is an intracellular iron storage protein that binds excess iron. The presence of ferritin can protect cells from iron-induced toxicity. In an experiment to investigate the effects of dietary iron intake on ferritin synthesis, rats were given food containing different amounts of iron. Subsequently, the levels of ferritin protein in the liver were measured. The results are shown in Figure 1. Based on these and other data, researchers have developed the following model demonstrating how ferritin synthesis is regulated by iron. When iron levels are low, a repressor of translation, iron response protein (IRP), binds to an iron response element (IRE), which is a stem-loop structure near the 5¢ end of ferritin mRNA. When iron levels are high, intracellular iron binds to the IRP, and the iron-IRP complex dissociates from the IRE, permitting ribosomes to proceed with the translation of ferritin mRNA. Figure 2 represents the model of the regulation of ferritin mRNA translation by iron. Based on the model of ferritin synthesis presented in Figure 2, which of the following best describes the mechanism whereby iron most likely regulates ferritin production?

Translation occurs under high intracellular iron concentration when the IRP-iron complex dissociates from ferritin mRNA, permitting ribosomes access to the ferritin coding region.

The Hedgehog protein (Hh) plays a critical role during a certain period of embryo development, but it normally has no role in adults except for the maintenance of adult stem cells. However, the Hedgehog protein has been detected in 70 percent of pancreatic cancer cell samples. As illustrated in the figures below, the Hedgehog protein binds to an integral membrane protein receptor known as Patched (Ptc), thus initiating a pathway of gene expression. When Hedgehog is absent, Ptc inhibits another protein known as Smoothened (Smo), which, in turn, blocks the activation of a group of proteins collectively known as the Hedgehog signaling complex (HSC). The inactivation is the result of proteolytic cleavage of one component of the HSC complex, a transcription factor known as Cubitus interruptus (Ci). When Hedgehog is present, it binds to Ptc, which prevents the inhibition of Smo by Ptc. The result is that Ci remains intact and can enter the nucleus, where it binds to and activates certain genes. One approach to treating patients with pancreatic cancer and other cancers in which the Hedgehog protein is detected is to modify the Hedgehog signaling pathway. Which of the following is the most useful approach?

Treating patients with a membrane-soluble compound that can bind to Smo and block its activity