BIO 100 Exam 2 Quizzes

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What are the four nitrogenous bases of DNA? Both A and B Adenine, Guanine, Cytosine, Thymine None of the above Adenine, Guanine, Uracil, Cytosine

Adenine, Guanine, Cytosine, Thymine

Which of the following best describes the organism used in class for DNA extraction? Eukaryotic, multicellular, presence of cell wall and organelles Prokaryotic, multicellular, presence of cell wall and organelles Eukaryotic, multicellular, absence of cell wall, organelles present Eukaryotic, unicellular, presence of cell wall and organelles Prokaryotic, unicellular, absence of cell wall and organelles

Eukaryotic, multicellular, presence of cell wall and organelles

According to the ACE reading "DNA exonerations" which of the following contributes to the most wrongful convictions? Eyewitness misidentification testimony. Improper forensic science. Informants. False confessions.

Eyewitness misidentification testimony.

According to one of the videos we watched in class, what leads to wrongful convictions? Eyewitness misidentification. Informant. Partial fingerprints. Crime committed by unknown identical twin. Contaminated forensic evidence.

Eyewitness misidentification.

According to the article "Forensics specialist discusses a discipline in crisis", which of the following techniques was declared inadmissible by the New York Supreme Court? Low - copy number DNA profiling. Ear - print evidence. Eyewitness identification. Restriction fragment length polymorphism. DNA fingerprinting.

Low - copy number DNA profiling.

According to the ACE reading "DNA exonerations", what constitutes unvalidated forensic science? Shoe print and bite mark comparisons only Hair microscopy. Shoe print comparisons, bite mark comparisons and hair microscopy. Shoe print comparisons. Bite mark comparisons. Bite mark comparisons and hair microscopy only.

Shoe print comparisons, bite mark comparisons and hair microscopy.

Which of the following options would tend to increase the speed at which DNA migrates in an agarose gel? increase the length of the fragment. Increase the amount of agar in the gel. Shorten the length of the fragment. increase the amount of buffer used to run the gel.

Shorten the length of the fragment.

What is the conclusion of the ACE class activity based on the DNA evidence we analyzed? Suspect 3 was the kidnapper. Results were inconclusive. Suspect 1 was the kidnapper. Suspect 8 was the kidnapper. Suspect 7 was the kidnapper.

Suspect 7 was the kidnapper.

Which sentence best describes the function of the first step during the DNA extraction (smashing and grinding)? To separate components of the cell. To break up proteins stuck to DNA. To precipitate DNA from solution. To dissolve the cell membrane. To break open the cells.

To break open the cells

What is the role of salt in DNA extraction? To precipitate DNA from solution. To break open the cells. To separate components of the cell. To break up proteins stuck to DNA. To dissolve the cell membrane.

To break up proteins stuck to DNA.

What is the role of detergent in DNA extraction? To separate components of the cell. To break open the cells. To break up proteins stuck to DNA. To dissolve the cell membrane. To precipitate DNA from solution.

To dissolve the cell membrane

What is the role of the ethanol in DNA extraction? To separate components of the cell. To dissolve the cell membrane. To break up proteins stuck to DNA. To precipitate DNA from solution. To break open the cells.

To precipitate DNA from solution

An AT rich genome is easier to degrade. True False

True

What is the charge of DNA? Neutral Multi-charge No charge Positive Negative

-ve

One liter (1000 ml) of DNA extraction buffer contains among other ingredients 50 ml of dishwasher detergent. What it s the final concentration of the detergent assuming the detergent is 100% pure? 5% 10% 25% 50% 150%

5%

Strawberries are octoploid, meaning they have eight copies of each type of chromosome. You repeat the experiment using the same amount in grams of bananas, a triploid organism. If you get 24 mg of DNA from the strawberry extraction, how many mg of DNA will you obtain using the banana? 6 mg 24 mg 3 mg 12 mg 9 mg

9 mg

According to the ACE reading "DNA exonerations", people that were wrongfully convicted were exonerated due to: Fingerprint analysis. DNA testing. Ballistics. Matching hair.

DNA Testing

What does DNA stand for? Deoxynitrogonous acetylcholine Deoxynucleic acid Deoxyribonucleic acid Do Not Attempt

Deoxyribonucleic acid

The extracted genetic material in class comes from what part of the cell? Cell wall Nucleus Cell membrane Nucleoid Cytoplasm

Nucleus

According to the ACE reading "DNA exonerations", eyewitness misidentification occurs in part because: People are not wearing glasses. People feel threatened by the suspect in the line-up. People are less able to recognize faces of a different race than their own. People forget very easily after a traumatic event.

People are less able to recognize faces of a different race than their own.

When doing a DNA gel electrophoresis like the one we did in class, based on the DNA charge, to which end does the DNA migrate to? Negative becasue DNA is positively charged due to the phosphate group. Positive because DNA is negatively charged due to the hydroxyl group. Positive because DNA is positively charged due to the phosphate group. Positive because DNA is negatively charged due to the phosphate group. Negative because DNA is positively charged due to hydroxyl group.

Positive because DNA is negatively charged due to the phosphate group

The technique we used to identify the kidnapper is called: Karyotyping. Restriction fragment length polymorphism. Low - copy number DNA profiling. High - copy number DNA profiling. Fluorescence in situ hybridization.

Restriction fragment length polymorphism


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