Bio 111 Cumulative Lab Quiz Questions

For the pipet above, what volume in microliters, will be dispensed? Do not include units in your answer. P-20 050 Lab Quiz 1

5

It is frequently necessary to perform dilutions of a stock solution in the laboratory. If you were instructed to make a 1:4 dilution of a stock solution to arrive at a final volume of 20, how much stock solution would you need to pipet? Lab Quiz 9

5

Select the wavelengths that will be tested for their effect on photosynthesis. More than one answer may be correct. Lab Quiz 5 A. Green light B. White light C. Red light D. Ultraviolet light D. X-rays E. Blue light

A, B, C, and E

Which of the following are true statements regarding safety in this week's lab? More than one answer may be correct. Lab Quiz 6 A. Sucking on hard candy in lab is ok. B. Arrive wearing close-toed shoes. C. Drinking water at the lab bench is ok, just avoid eating a snack in lab. D. Pull back long hair.

Arrive wearing close-toed shoes and Pull back long hair.

What is the function of the Chelex beads? Lab Quiz 6

Bind metal ions so they can be removed from the sample.

What is the purpose of PCR? Lab Quiz 6 A. Make many, many copies of a specific region of RNA B. Create new DNA that is more stable than genomic DNA C. Make many, many copies of a specific region of DNA D. Translate a region of DNA into a great deal of protein

C. Make many, many copies of a specific region of DNA

What is the function of the thermocycler? Lab Quiz 6

Can be programmed to keep samples/reactions at specific temperatures for specific lengths of time.

Experiment 1 this week will examine the effect which substrate [a] has on enzyme [b]. Experiment 2 will instead vary [c] concentration. Both experiments will utilize the [d] to measure changes in [e]. Lab Quiz 3 Activity, Absorbance, Concentration, Enzyme, Catalyst, Mass, Movement, Substrate, Bacteria

Experiment 1 this week will examine the effect which substrate [Concentration] has on enzyme [Activity]. Experiment 2 will instead vary [Enzyme] concentration. Both experiments will utilize the [Spectrophotometer] to measure changes in [Absorbance].

What are the steps of PCR? Lab Quiz 6 Denature, Extend, Assemble reaction with template DNA and master mix, Anneal

1. Assemble reaction with template DNA and master mix 2. Denature 3. Anneal 4. Extend

Put the steps of the central dogma of molecular biology in order including the molecules and the processes that create one from the other. Lab Quiz 9 DNA, Protein, Translation, RNA, mRNA, Transcription

1. DNA 2. Transcription 3. mRNA 4. Translation 5. Protein

Place the following steps of aerobic respiration in order. Lab Quiz 4 A. Oxidation of pyruvate B. Krebs/citric acid cycle C. Electron transport chain D. Glycolysis

1. Glycolysis 2. Oxidation of pyruvate 3. Krebs/citric acid cycle 4. Electron transport chain

Place the steps of measuring a sample's absorbance in order from 1-5. Lab Quiz 3 A. Adjust absorbance/transmittance dials till the readings are 0. (Then remove cuvette.) B. Set wavelength on spectrophotometer C. Wipe the cuvette containing the sample and insert it into the spectrophotometer D. Record absorbance reading E. Wipe the cuvette containing the blank and insert it into the spectrophotometer.

1. Set wavelength on spectrophotometer. 2. Wipe the cuvette containing the blank and insert it into the spectrophotometer. 3. Adjust absorbance/transmittance dials till the readings are 0. (Then remove cuvette.) 4. Wipe the cuvette containing the sample and insert it into the spectrophotometer. 5. Record absorbance reading.

For the pipet above, what volume in microliters, will be dispensed? Do not include units in your answer. P-200 1-1-8 Lab Quiz

118

For the pipet above, what volume in microliters, will be dispensed? Do not include units in your answer. (Disregard the picture) Lab Quiz 1 Pipette reads P-200 1 2 5

125

Match the item or reagent to the proper disposal container for this week's experiments. Lab Quiz 6 A. 15 mL tube for pelleting cells B. Pipette tips C. Spit cups D. Gloves

15 mL tube for pelleting cells-Biohazard waste Pipette tips-Biohazard waste Spit cups-Biohazard waste Gloves-Trash can

Cells from an organism were observed under the microscope and the cell cycle status of each cell was recorded as in the table below. Lab Quiz 8 Phase # cells % of cells Interphase 657 86.8% Prophase 79 10.4% Metaphase 11 1.5% Anaphase 6 0.8% Telophase 4 0.5% Total 757 Approximately how many hours are these cells in prophase?

2.4

Based on the phenotypes of the parental plants and F1 plants from the Heredity I lab, which phenotypes are dominant? More than one answer may be correct. A. GFP positive B. Trichomes C. No GFP D. Glabrous

A and B

Which statements about genetics and heredity are TRUE? More than one answer may be correct. A. The genotypic and phenotypic frequencies in a cross between AaBb x AaBb are not the same. B. Genotype refers to the alleles an individual possesses. C. The genotypic and phenotypic frequencies in a cross between AaBb x AaBb are always the same. D. The genotype of a random Arabidopsis plant can be easily determined by observation with tools like compound scopes and a light to check for fluorescence.

A and B

Which of the following would not be expected to have an effect on the activity of ß-galactosidase? More than one answer may be correct. Lab Quiz 3 A. Absorbance B. ß-galactosidase C. BSA Concentration D. Temperature E. ONPG (ortho-nitrophenol-ß-galactoside) concentration F. pH

A and C

Which of the following statements are TRUE regarding footwear in BIOL 111 labs? More than one answer may be correct. Lab Quiz 1 A. You may lose TA points if you do not arrive wearing closed-toed shoes in lab. B. Sandals are acceptable if outdoor temperatures are above 85F. C. Shoes with a peep toe are fine as long as the majority of your foot is covered. D. Shoes must cover your toes and a majority of your foot.

A and D

Which of the following will be components in your PCR mixture? More than one answer may be correct. Lab Quiz 6 A. Master Mix B. E. coli DNA C. Bradford Reagent D. Human DNA

A and D

Which of the following are true statements about Arabidopsis? More than one answer may be correct. Lab Quiz 10 A. It is possible to create plants that carry genes not normally found in Arabidopsis. B. It has long generation times. C. It is a model organism. D. Arabidopsis is easy to grow in a lab.

A, C, and D

If you allow a heterozygous tall plant that is heterozygous for purple flowers to self-pollinate, how many tall and purple plants would you expect in the F2 generation if you sample 16 individuals? Both tall and purple flowers are dominant. Lab Quiz 10 A. 9 B. 3 C. 1 D. 4 E. 0

A. 9

You are preparing to set up a Punnett square for the cross, AaBb x aabb. The gametes for the underlined parent would be: Lab Quiz 8 A. AB, Ab, aB, ab B. AA, BB, aa, bb C. Aa, Bb, AA, BB, bb, aa D. AABB, AAbb, aaBB, AaBb

A. AB, Ab, aB, ab

Which of the following statements about cellular respiration are TRUE? More than one answer may be correct. Lab Quiz 4 A. Anaerobic respiration produces fewer ATP than aerobic respiration. B. Fermentation is most commonly utilized when cells have plenty of O2 available. C. Aerobic respiration is advantageous because it does not require the presence of O2. D. Anaerobic respiration utilizes NAD+ as the final electron acceptor.

A. Anaerobic respiration produces fewer ATP than aerobic respiration.

This cell is in: Lab Quiz 8 A. Metaphase B. Anaphase C. Cytokinesis D. Interphase E. Prophase

A. Metaphase

James struggled in his first semester and ended up Q-dropping his BIOL111 class. He seems to have things turned around and is taking BIOL111 again in the spring semester. James did pretty well in lab his first time through, so when lab starts up, he just submits his old lab reports that he scored well on instead of redoing the assignment. Which of the following types of academic dishonesty did James commit? A. Multiple submissions B. Falsification C. Plagiarism D. Complicity E. Fabrication

A. Multiple Submissions

In the lac operon, the operator functions to Lab Quiz 9 A. bind the repressor and prevent lac gene expression. B. binds to the promoter to promote lac gene expression. C. prevent the repressor from binding and allow lac gene expression. D. bind RNA polymerase and block lac gene expression.

A. bind the repressor and prevent lac gene expression.

In animals, what type of sequence is commonly used to construct a phylogeny and determine ancestry? Lab Quiz 7 A. mitochondrial DNA B. telomeric DNA C. plasmid DNA D. centromeric DNA

A. mitochondrial DNA

Based on the DNA ladder in lane 1, select the band containing DNA that is 700 bp long. (refer to the picture) Lab Quiz 7 A B C D

B

Of the following, which are sequences you can search the databases of GenBank with? More than one answer may be correct. Lab Quiz 7 A. Fatty Acid B. DNA C. Protein D. Carbohydrate

B and C

Which genotypes are possible from a cross of parents with the genotypes Dd x DD? More than one answer may be correct. Lab Quiz 8 A. dd B. Dd C. DD D. DDDd

B and C

This week's procedures will include (More than one answer may be correct): Lab Quiz 10 A. Plating fertilized seeds to examine an F3 generation. B. Examining plants under blue light to look for GFP. C. Examining P generation plants to determine inheritance pattern of GFP. D. Examining plants under the dissecting scope to look for trichomes.

B and D

How will the bands on the gel be visualized? Lab Quiz 7 A. The bands are visible with the naked eye and no special equipment. B. A UV light will make the fluorescent dye attached to the DNA glow. C. Green light in a light box will activate dye attached to the DNA. D. The bands will be stained after the gel is finished running so they will be visible to the naked eye.

B. A UV light will make the fluorescent dye attached to the DNA glow.

If the graph represents the change in β-galactosidase production (Y-axis) in E.coli cells over time (X-axis), which of the following is true? Lab Quiz 9 I- The amount of allolactose is highest at B and E II- The amount of allolactose is highest at D and G III- The amount of active repressor is highest at D and G IV- The amount of active repressor if highest at B and E A. Only I B. I and III C. Only IV D. II and IV

B. I and III

During the process of glycolysis, the potential chemical energy released by the breakdown of glucose is stored as Lab Quiz 4 A. ADP and NAD+ B. NADH and ATP C. CO2 and H2O D. Pyruvate

B. NADH and ATP

This cell is in: Lab Quiz 8 A. Anaphase B. Prophase C. Metaphase D. Cytokinesis E. Interphase

B. Prophase

What is bioinformatics? Lab Quiz 6 A. The use of molecular techniques to investigate how biological molecules store information. B. The application of computer technology and associated software to biological data. C. A tool for analyzing sequence similarity built by the NCBI (National Center for Biotechnology Information). D. Information about biology.

B. The application of computer technology and associated software to biological data.

At the conclusion of this experiment, all used culture materials should be placed in what location? Lab Quiz 9 A. The liquid cultures should be poured back into the original culture flasks. B. The liquid waste disposal container on the side bench. C. Down the sink drain, flushed with plenty of water. D. They should be left on the bench for the lab instructor to deal with.

B. The liquid waste disposal container on the side bench.

In a prokaryotic operon, the promoter functions to Lab Quiz 9 A. break down lactose. B. bind the RNA polymerase. C. prevent the expression of lac genes. D. bind the repressor.

B. bind the RNA polymerase.

In the lac operon, the repressor Lab Quiz 9 A. binds to allolactose and becomes active, blocking lac gene expression. B. binds to allolactose and becomes inactive, allowing lac gene expression to occur. C. binds allolactose and binds to the promoter, and induces lac gene expression. D. binds to the promoter and blocks lac gene expression.

B. binds to allolactose and becomes inactive, allowing lac gene expression to occur.

All used pipet tips and microcentrifuge tubes should be disposed of Lab Quiz 9 A. by leaving them on the lab bench for the lab instructor to dispose of. B. in the waste bag/bucket on my bench. C. back into the container where we got them. D. in the trash can.

B. in the waste bag/bucket on my bench.

What does PCR stand for? Lab Quiz 6 A. Polyclonal coli reaction B. Polymerase chain reaction C. Pretty cool reaction D. Phosphate chlorate reaction

B. polymerase chain reaction

When you arrive at lab, which of the following do you need to be wearing? Lab Quiz 3 A. Goggles B. Gloves C. Closed-toed Shoes D. Lab Coat

C. Closed-toed Shoes

Ortho-nitrophenol production can be measured with a spectrophotometer due to which of the following characteristics? Lab Quiz 3 A. Mass B. pH C. Color D. Temperature

C. Color

This cell is in: Lab Quiz 8 A. Anaphase B. Prophase C. Cytokinesis D. Interphase E. Metaphase

C. Cytokinesis

When heterozygous tall plants with purple flowers self pollinate to give rise to tall and dwarf plants with purple flowers, it demonstrates the following: Lab Quiz 10 A. Independent assortment of alleles B. Co-dominance of alleles C. Segregation of alleles D. Dependent assortment of alleles

C. Segregation of alleles

Which of the following are true about spectrophotometers? More than one answer may be correct. Lab Quiz 3 A. Can measure how much light is absorbed by a solution. B. Measures the mass of solutes in a solution. C. Can measure how much light passes through the sample (transmittance). D. Works by shining light of a specific wavelength through the sample.

Can measure how much light is absorbed by a solution, Can measure how much light passes through the sample (transmittance), and Works by shining light of a specific wavelength through the sample.

Which of these statements are good examples of following lab safety protocols? More than one answer may be correct. Lab Quiz 3 A. Reapplying lip balm at the lab bench. B. Removing your gloves while pipetting so you have better dexterity. C. Chewing gum while setting up your lysis reactions. D. Asking the TA if you can step outside to get a drink of water.

D. Asking the TA if you can step outside to get a drink of water.

Because the Arabidopsis are transgenic (they have had genetic material added/modified), what is the proper disposal procedure? Lab Quiz 10 A. Planting outside In the tip bucket C. Tash can D. Biohazard disposal

D. Biohazard disposal

Which type of microscope is best for observing presence/absence of trichomes? Lab Quiz 8 A. Confocal B. Compound C. Scanning Electron D. Dissecting

D. Dissecting

Which bacteria is the source of the enzyme used in this week's experiments? Lab Quiz 3 A. Streptococcus pyogenes B. Helicobacter pylori C. Escherichia vulneris D. Escherichia coli

D. Escherichia coli

Which of the following values show how closely matched your sequence is to potential matches from the database? More than one answer may be correct. Lab Quiz 7 A. Identity score B. 16S score C. E-value D. Max score

D. Max score

What are trichomes in reference to plants? Lab Quiz 8 A. The pores through which gas exchange happens for photosynthesis B. The very tips of the fine root hairs C. Where a plant does most of its vertical growth D. Small hairs projecting from the leaves

D. Small hairs projecting from the leaves

Which microorganism will be used in the fermentation experiments? Lab Quiz 4 A. Bacteria B. Mold C. Protozoa D. Yeast

D. Yeast

Based on the phenotypes of the P generation and F1 generation, what are possible phenotypes in the F2 generation? More than one answer may be correct. Lab Quiz 10 A. Trichomes, no GFP B. Glabrous, no GFP C. Trichomes, GFP positive D. Glabrous, GFP positive E. All of the above

E. All of the above

Select the safety statements which apply to this lab. More than one answer may be correct. Lab Quiz 7 A. Loose/dangling clothing/accessories should be removed or contained so they will not fall into experiments. B. Goggles must be on and covering eyes when any experiments are being done in the lab. C. There are reagents and supplies that require disposal in biohazardous waste. D. Arrive at lab already wearing close-toed shoes. E. All of the above

E. All of the above

Which of these statements are true about micropipettors? More than one answer may be correct. Lab Quiz 3 A. The second stop is used to dispense fluid from the tip. B. Dispense very small and precise volumes of fluid. C. The first stop is used to draw the desired volume into the tip. D. Have a specific range of volumes which each size micropipette can handle. E. All of the above

E. All of the above

Joanna takes the online lab exam when it first opens. Rachel later posts a question in the GroupMe chat about what she should study. Joanna runs through an overview the exam and even mentions some of the questions specifically. Rachel is surprised by the response and reports it to her TA. Lab Quiz 1 Which of the following types of academic dishonesty did Joanna commit? A. Plagiarism B. Fabrication C. Falsification D. Multiple Submissions E. Complicity

E. Complicity

Which of the following are not allowed in lab? More than one answer may be correct. Lab Quiz 4 A. Applying cosmetics (including lip balm) B. Drinking water (or anything else) C. Eating food D. Chewing gum E. All of the above

E. all of the above

Enzymes bind [a], which fit into a spot called the [b]. The enzyme then [c] a biochemical reaction, [d] the rate at which the reaction takes place. Lab Quiz 3 Active Site, Substrates, Speeding Up, Slowing Down, Catalyzes, Decreases, Passive Site, Concentration

Enzymes bind [Substrates], which fit into a spot called the [Active Site]. The enzyme then [Catalyzes] a biochemical reaction, [Speeding Up] the rate at which the reaction takes place.

Gel [a] uses an electrical charge to move [b] through a gel matrix made of [c]. This causes fragments to be separated by [d]. Lab Quiz 7 Electrophoresis, Size, pH, RNA, DNA, Color, Agarose

Gel [Electrophoresis] uses an electrical charge to move [DNA] through a gel matrix made of [Agarose]. This causes fragments to be separated by [Size].

What is the correct disposal location for each of the following waste items? Answer choices may be used once, more than once, or not at all. Lab Quiz 7 Gloves Agarose Gels Tubes Paper Towels Tips

Gloves-Trashcan Agarose Gels-Biohazard Waste Tubes-Tip Bucket Paper Towels-Trashcan Tips-Tip Bucket

In [a], energy from sunlight is used to extract electrons from [b]. The electrons travel through the [c] to [d], which reduces [e] to [f]. Lab Quiz 5 H2O (Water), Oxygen, CO2, Glucose, NADPH, NADP+, ATP, Electron Transport Chain, Photosystem I, Photosystem II

In [Photosystem II], energy from sunlight is used to extract electrons from [H2O (Water)]. The electrons travel through the [Electron Transport Chain] to [Photosystem I], which reduces [NADP+] to [NADPH].

In general, a slight increase in temperature will [a] the rate of cellular respiration. This is most directly due to [b] increasing in [c] conditions. Lab Quiz 4 Warmer, Cooler, Enzyme activity, Concentration, pH, H+, Color, Temperature, Speed up, Slow down, Accelerate, Decelerate, Constant

In general, a slight increase in temperature will [Accelerate] the rate of cellular respiration. This is most directly due to [Enzyme activity] increasing in [Warmer] conditions.

What is the function of the micropipette? Lab Quiz 6

Measures and transfers very specific, usually small, volumes of fluid accurately.

Photosynthesis results in the production of [a], which is the primary energy source in cells. The byproduct of photosynthesis is [b]. Lab Quiz 5 Oxygen, Carbon Dioxide, Water, Glucose

Photosynthesis results in the production of [Glucose], which is the primary energy source in cells. The byproduct of photosynthesis is [Oxygen].

Which of the following are good routine practices to maintain safety in the lab? More than one answer may be correct. Lab Quiz 1 A. Pushing your lab stool in under the desk when not in use. B. Using a closable sippy cup for your water while in lab. C. Keeping food and drink out of the lab or stored securely inside a backpack. D. Placing backpack and bags in the cubby or on the hook in the knee hole.

Pushing your lab stool in under the desk when not in use, Keeping food and drink out of the lab or stored securely inside a backpack, and Placing backpack and bags in the cubby or on the hook in the knee hole.

Match the following components to their role in the experiment. Lab Quiz 4 Soaked beans Dry beans Water bath Respirometer

Soaked beans-Experimental sample Dry beans-Control Water bath-Keeps samples at a constant temperature Respirometer-A tool to measure the consumption of gas

What is the function of the centrifuge? Lab Quiz 6

Spins at a high speed to separate things by density (denser stuff moves lower in the tube).

What is denaturation? Lab Quiz 6

The DNA template is heated so the strands separate.

Match the following terms based on this week's experiments: Lab Quiz 3 The substrate is The colorless product is The enzyme is The product which you will measure via spectrophotometry is

The Substrate: ONPG (ortho-nitrophenyl-beta-galactoside) The colorless product: galactose The enzyme: Beta-galactosidase The product which you will measure via spectrophotometry: ortho-nitrophenol

The [A] assay can be used to determine the expression of [B] under different environmental conditions. If the gene is expressed and the [C] is present, [D] will be broken down and create a [E] pigment. Lab Quiz 9 Red, Orange, Yellow, Green, Blue, Purple, Substrate, Enzyme, ONPG, β-gal, LacZ

The [β-gal] assay can be used to determine the expression of [LacZ] under different environmental conditions. If the gene is expressed and the [Enzyme] is present, [ONPG] will be broken down and create a [Yellow] pigment.

What is a thermocycler? Lab Quiz 6

The machine that controls the temperature of the reaction and has been programmed to move through the heating and cooling steps.

What is annealing? Lab Quiz 6

The reaction is cooled so the primers can stick to the DNA template.

What is extension? Lab Quiz 6

The temperature is changed to the ideal temperature for Taq polymerase to build (synthesize) a new strand based on the template.

This week's experiment will examine cellular respiration by measuring [x] consumption. Meanwhile, any [y] produced by the cells will react with KOH (potassium hydroxide) to form non-gaseous molecules. Lab Quiz 4 CO2 (carbon dioxide), C6H12O6 (glucose), O2 (oxygen), H2O (water), ADP, NAD+, NADH, ATP

This week's experiment will examine cellular respiration by measuring [O2 (oxygen)] consumption. Meanwhile, any [CO2 (carbon dioxide)] produced by the cells will react with KOH (potassium hydroxide) to form non-gaseous molecules.

This week, two generations of plants will be observed: the [a] that were crossed to create the first generation and those offspring, called the [b] generation. The seeds that you will plate are the [c] generation. Lab Quiz 8 F1,F2,Brothers, Sisters, Parents, Siblings, Younger, Older

This week, two generations of plants will be observed: the [Parents] that were crossed to create the first generation and those offspring, called the [F1] generation. The seeds that you will plate are the [F2] generation.

When migrating through the [a], [b] fragments of [c] can move quickly so will be found [d] from/to the wells than [e] fragments. Lab Quiz 7 Small, Large, Farther, Closer, DNA, RNA, Protein, Agarose

When migrating through the [Agarose], [Small] fragments of [DNA] can move quickly so will be found [Further] from/to the wells than [Large] fragments.

When you are doing an experiment, your hands should be covered by [x] and your [y] should be covering your eyes. Goggles which are propped on your [z] and gloves which are still in their [a] will not protect you from chemicals. Lab Quiz 5 Goggles, Lab coat, Close-toed shoes, Forehead, Neck, Arm, Bag, Gloves, Dirt, Box, Container

When you are doing an experiment, your hands should be covered by [Gloves] and your [Goggles] should be covering your eyes. Goggles which are propped on your [Forehead] and gloves which are still in their [Box] will not protect you from chemicals.

[a] is an overarching term that includes all processes living organisms use to produce [b]. Lab Quiz 4 CO2, C6H12O6, O2, H2O, ADP, NAD+, NADH, ATP, Cellular Respiration, Photosynthesis, Glycolysis, Hydrolysis, Krebs/citric acid cycle, Fermentation

[Cellular Respiration] is an overarching term that includes all processes living organisms use to produce [ATP].