BIO 335 - Methods in Molecular Genetics
What is a microsatellite?
A short DNA sequence repeated many times in a row
During a researchers in situ hybridization procedure, what causes the probe to stick to the chromosome?
Complementary base pairing in the corresponding gene
The most common method of in situ hybridization uses ______ DNA probes
Fluorescent
The mapping technique that relies on the frequency of recombinant offspring for determination of the distance between genetic loci is ______ mapping
Linkage
A short repetitive DNA sequence, such as dinucleotide sequence repeated many times in a row is called
Microsatellite
A segment of DNA that is located at a specific site along a chromosome and that has properties that allow it to be uniquely identified using molecular tools is called a ______ marker
Molecular
One of the benefits of sequencing the human genome is that it will make it easier to identify genes that cause disease when they are
Mutated
A microsatellite can be amplified by PCR using primers that are located
On both sides of the microsatellite
cytogenetic mapping
Determination of the locations of specific sequences relative to bands on chromosomes
physical mapping
Determining the locations of genes and other DNA sequences using DNA cloning techniques
linkage mapping
Determining the relative locations of genes on a chromosome based on their frequency of genetic recombination
What is the goal of genome sequencing project?
To determine the DNA sequence of the entire genome of a given species
The ability to rapidly sequence large amounts of DNA is called _____ - ______ sequencing
High throughput
One of the goals of the human genome project was to obtain a genetic linkage map of the human genome. This was accomplished by
Identifying millions of genetic markers and their locations on chromosomes
What outcomes have already resulted from the human genome project?
Improved DNA sequencing technology Improvements in software for accessing and analyzing sequence DNA Improvements in cloning technology Sequence data for the 22 human autosomes Sequence data for the human X and Y chromosomes
