Bio lab I
Hydrogen peroxide is broken down to produce water and oxygen with the help of the enzyme catalase. Which of the following can be measured to determine the enzymatic activity? Select all that apply.
how much hydrogen peroxide is used Correct! how much water is produced Correct! how much oxygen is produced
Which of the following unexpected results could occur if you used the catalase before it had a chance to reach your experimental temperature? Select all that apply.
in the experiment with the cooled enzyme, you would get a higher bubble column that expected in the experiment with the over heated enzyme, you would get a higher bubble column than expected
In the enzyme experiment, what was the purpose of preparing a tube with water and hydrogen peroxide?
it is a negative control
If you have a constant amount of substrate and add an increasing concentration of enzyme, what will happen to the rate of reaction?
it will increase
Which of the following determines the quality and clarity of the microscope image? Select all that apply.
magnification contrast resolving power
Increasing the concentration of enzyme results in __ substrate interacting with the enzyme per unit volume.
more
Where are the pill bugs organs of smell?
on the antennae
Phospholipids have a similar structure to fats except they contain a __ group in place of the third fatty acid.
phosphate
When should the lab bench be cleaned and disinfected? Check all that apply.
prior to the lab activity Correct! after the completion of the lab activities
Which topics belong in a scientific paper? Select all that apply.
procedures Correct! results introduction
A positive reaction for the presence of protein using the Biuret test would be __ in color.
purple
Which of the following colors indicates a positive reaction for the presence of reducing sugar using Benedict's test?
red
The functions of lipids include which of the following? Select all that apply.
storage of energy Correct! construction of membranes
Besides storing carbon, having structural functions and being involved in communication, what other role do carbohydrates have?
storing energy
In the enzyme experiment, what will happen if the test tubes are left an hour instead of 20 seconds? Select all that apply.
the bubbles will burst Correct Answer the tubes will all have the same height bubbles the bubbles will no longer be formed
At very high temperatures enzymes can exhibit less activity or none at all. Which of the following could explain this? Select all that apply.
the enzyme has denatured and cannot interact with the substrate the active site has changed shape so that the substrate can not fit
How are the metric units of measurement different that the standard system used in the U.S.? Select all that apply.
the metric system is used in more countries worldwide metric units are based on multiples of 10
What are the advantages of using a wet mount?
the motility of a specimen can be viewed under the microscope The specimen can be viewed as living cells
Which of the following techniques can be used to view living specimens? Select all that apply.
wet mount simple stain
Why is it important to perform an experiment with a control? Correct!
without a control, a positive test could have been explained by some other factor causing a false positive Correct Answer without a control, a negative test could have been explained by some other factor causing a false positive
Three test tubes are set in a rack. They contain the following: Tube 1: 4 cm of hydrogen peroxide & 3 cm catalase Tube 2: 4 cm of hydrogen peroxide & 5 cm catalase Tube 3: 4 cm of hydrogen peroxide & 7 cm catalase In which tube will you see the highest column of bubbles?
3
58.6 centimeters corresponds to __ millimeters.
586
How many pairs of legs does a pill bug have?
7
An enzyme at its optimum pH will produce a column of bubbles that is higher than when it is not at its optimum pH because it is less active.
False
To determine the activity of an enzyme, you can compare the concentration of the enzyme before and after the chemical reaction.
False
When the enzyme catalase is mixed with the substrate water, oxygen bubbles are produced.
False
Consider the following experiment: Test tube 1 contains 1 mL of catalase and 1 mL of hydrogen peroxide. Test tube 2 contains 1 mL of catalase and 2 mL of hydrogen peroxide. Test tube 3 contains 1 mL of catalase and 4 mL of hydrogen peroxide. If the concentration of the substrate is the limiting reaction rate, which tube is expected to produce the highest column of bubbles?
Tube 3
The __ __ preparation allows one to observe the behavior and movement of microorganisms with a light microscope. (two words)
WET MOUNT
Which type of appendage is used by the pill bug to explore its environment?
antennae
How many pounds are in 1 gram?
0.0022 lbs
How many cubic centimeters is 1 milliliter?
1
The pH of an enzyme solution can alter the shape of the enzyme and its active site by affecting __ bonding.
Ionic
Which of the following are found in both plant and animal cells?
Nuclei
Plants store glucose as __.
Starch
In the enzyme experiment, how was the temperature of the catalase reaction controlled?
catalase samples are either heated or cooled
In addition to storing carbon and energy, what else are lipids used for in cells?
constructing membranes
