Blood Bank Lab CE questions
What is the rare phenotype found exclusively in male patients that is caused by X-linked inheritance from a carrier mother, often demonstrating a chronic but well-compensated anemia as well as muscle and nerve disorders?
McLeod
If an individual had blood type O, what is their genotype?
OO
Which of the following noninfectious complications of blood transfusion is prevented by the irradiation of blood components?
TA-GVHD
The plant lectin obtained for the dolichos biflorus plant will agglutinate which of the following?
A1 cells
Which blood groups reacts least strongly with anti-H?
A1B
When processing umbilical cord blood samples for hematopoietic progenitor cells (HPC), what tests are performed on both the mother's blood and cord blood?
ABO and Rh
When giving whole blood to a patient, what consideration must be made in regard to the ABO/Rh compatibility between the recipient and the donor?
ABO and Rh must be identical
In the antibody screening test, at what phase of reactivity is it most important to read the reactions of clinically significant antibodies?
AHG
The "recognition unit" of the classical complement pathway refers to which of the following?
C1q
A low-incidence antigen is one that occurs in less that 10% of the population. Which of the following is a low-incidence antigen?
C^w
Which of the following would be a possible cause of a positive direct antiglobulin test (DAT)?
Clot tube stored at 4oC, transfusion reaction, and warm autoimmune disease.
Which of the following is used as a source for irradiation of blood products?
Cs^137
Cytomegalovirus (CMV) is a widespread infection that can be transmitted through transfusion. Units that test negative for CMV are set aside for intrauterine transfusion or blood replacement for premature infants and immunocompromised adults. CMV-reduced-risk blood products can also be achieved by leukocyte reduction because the virus resides within intact white cells.
*Just a tid bit of info regarding last question*
Irradiation is performed using cesium-137 or cobalt-60. To confirm a product was irradiated, a radiochromic film label is affixed to the component before it is placed into the metal canister of the irradiator. Darkening of the film confirms irradiation requirements.
*Tid bid info for last question*
Which of the following is the proper storage temp for FFP?
-20C
A refrigerator is used to store whole blood must be able to maintain a temp range of what?
1-6 C
At what temperature should whole blood be stored?
1-6 C
When administering FFP, which one of the following is considered standard blood bank practice?
should be ABO compatible with patient RBC
An Rh negative mother has just given birth to an Rh positive baby. Her physician suspects that she has experienced a fetal-maternal hemmorhage since her rosette test was positive. Upon performing the Kleihauer-Betke stain procedure, the percentage of fetal cells is found to be 0.85%. The mother's total blood volume is 4,565 mL. What dose of Rh Immune Globulin (RhIG or RhoGam) should be administered to the mother?
2 vials
Rejuvenation solutions for donated red blood cell units may be used to improve what factor that is affected by storage lesion?
2,3 DPG
Platelets should be stored at what temperature?
20-24 C
In which of the following sections of CFR title 21, good manufacturing practices, would you find requirements for quality control testing of donor units of platelets?
21 CFR 640.25 Platelets- General requirements
Red blood cell units that contain CPD can be stored for up to how many days?
21 days
How soon must granulocyte concentrates be administered after donation?
24 hrs
What is the minimum number of reactive antigen-positive and non-reactive antigen-negative panel cells that must be present in order to identify a red cell antibody with statistical confidence?
3
What is the expected life span of a normal rbc?
3-4months
What is the minimum acceptable hematocrit value for a female who wants to donate an autologous unit of blood for an upcoming surgery?
33%
Donated RBC coonatin CPDA-1 may be stored how many days?
35 days
An Rh negative mother has just given birth to an Rh positive baby after 18 hours of strenuous labor. Her rosette test was positive. Upon performing the Kleihauer-Betke stain procedure, the percentage of fetal cells is found to be 1.9%. The mother's total blood volume is 5,000 mL. What dose of Rh Ig (RhoGam) should be administered to the mother?
4 vials
What is the maximum number of white cells allowed in a leukocyte-reduced unit of rbc
5X10^6
A standard blood donation of 450-500 mL requires a minimum anticoagulant volume of?
63mL
The A1 subgroup represents approximately what percentage of group A individuals?
75-80%
Platelets that are not collected by an apheresis method must be prepared within ________ of the collection of whole blood.
8 hrs
The accepted interval between whole blood donations is
8 weeks or 56 days
Which of the following represents the approximate percentage of the caucasian population that is rh positive?
85%
What does the hh genotype gives rise to?
Bombay phenotype
When a group O patient's serum is not compatible with donor O red blood cells and the auto control is negative what is most likely the cause?
Bombay phenotype
The immediate spin phase of an indirect antiglobulin test (antibody screen and antibody identification) could most likely lead to the detection of which of the following?
clinically insignificant cold antibodies
Which of the following genotypes are known to be resistant to P. vivax (malaria) merozoites?
Fy(a-b-)
In which system are DR antigens found?
HLA system
An antibody to which platelet antigen is most commonly encountered in blood bank?
HPA-1a
Which of the following choices is the basic technique used to detect unexpected antibodies with a commercial set of reagent cells?
IAT
Patients with which of the following conditions would benefit most from washed red cells?
IgA deficiency with anti-IgA
The immunoglobulin class responsible for the etiology of Hemolytic Disease of the Fetus and Newborn (HDFN) is
IgG
ABO antibodies are classified as which of the following 2 immunoglobulin classes?
IgG and IgM
Coombs control check cells used to verify negative reactions at the anti-globulin (IAT) phase are coated with:
IgG antibodies
A DAT can be used to detect all of the following except?
IgG antibodies in the patient's serum
which of the following is the predominant immunoglobulin class for anti-A and anti-B antibodies in group b and group a individuals?
IgM
The addition of Low Ionic Strength Solution (LISS) to the testing environment when performing an indirect antiglobulin test is designed to do what?
LOWERING THE ZETA POTENTIAL
Which of the following is considered a high-incidence antigen?
Little k
Which of the following conditions is most frequently associated with anti-I?
cold agglutinin disease
Which is the best component to treat a patient with fibrinogen deficiency?
cryo
Which one of the following blood components contains the most Factor VIII concentration?
cryo
What must be true for the antiglobulin phase of the serologic crossmatch to be omitted (i.e., immediate spin crossmatch is done)?
the antibody screen must be negative and no history of unexpected antibodies
Which organism is MOST likely responsible for septic reactions associated with red blood cell transfusions?
Yersinia centercolitica
Which of these units of RBC, that has been issued and returned to blood bank, can be returned to inventory and is acceptable to use
a unit of RBC that was not transfused but stored in a temperature-controlled remote refrigerator in the operation room for 2 hrs
An exchange transfusion has been ordered for a low birth weight infant. The physician has ordered irradiated Red Blood Cells for this purpose. Why is it necessary to irradiate the blood products for this patient?
to prevent TA-GVHD
What is considered the cause of urticarial transfusion reactions?
transfusion of certain food allergens or drugs in plasma products
Which of the following is the most important factor in determining the immediate outcomes of kidney transplants?
abo compatibility
Antibodies directed against granulocyte antigens have been implicated for all of the following except?
acute hemolytic transfusion reactions
Both hemoglobinuria and hemoglobinemia will be present in what type of reactions?
acute hemolytic transfusion reactions
The AABB Standards for Blood Banks and Transfusion Services requires a control system for antiglobulin tests interpreted as negative. What is done for this control system?
adding IgG sensitized red cells
Which of the following individuals is a candidate to receive RhIg?
an Rh negative mother upon delivery of first child
Which of the following antibodies is the MOST common cause of severe hemolytic disease of the newborn?
anti-D
What antibody is best described as common autoantibody that can be found in virtually all sera?
anti-I
If detected in antibody screen testing, which of the following antibodies is not considered clinically significant in prenatal patients?
anti-LeB
Which of the following best describes a transfusion reaction?
any adverse event associated with transfusion of blood components
Leukocyte reduction may be performed at the same time as which step in blood component collection?
at the same time the additive solution is added
A 9-year-old male in Alaska presents with hemolysis and hematuria. The patient history reveals that he recently recovered from a flu-like virus. The ABO type is A+, and the DAT reacts at 1+. What blood group antibody should be suspected?
autoanti-P
Which of the following can be used to confirm if someone has the bombay phenotype?
ulex europaeus
Which of the following is most commonly encountered presenting sign of a delayed hemolytic transfusion reaction?
unexplained decrease in H&H
All of the following are appropriate indications for the use of FFP except:
volume expansion
If Jka is showing dosage, how might reactions on an antibody panel appear
weaker is heterozygous
Transfusion of which blood product is most likely to cause circulatory overload in patients?
whole blood
All of the following serological test results may occur in a patient experiencing a hemolytic transfusion reaction due to the presence of a clinically significant Rh antibody such as anti-c except:
binding complement
Which type of blood component is most implicated in bacterial contamination?
platelets
A 40-year-old female receives two units of Red Blood Cells during a surgical procedure. The patient has no prior history of transfusions. Seven days later, she presents with extensive bruising of the extremities and bleeding of the gums, with no additional symptoms. Her platelet count is 5 x 109/L (reference interval 150 - 400 x 109/L). What is the most likely diagnosis?
post transfusion purpura (PTP)
In which situation is gamma irradiation of cellular blood components required?
prevention of graft-versus-host disease
What is characteristic of a person who is weak D?
demonstrates a reduced number of D antigen sites on the surface of the red cell
platelet products will be prepared from a whole blood donation. what must be used to prevent potential contamination with skin cells and bacteria?
diversion pouch
Which of the following is the most common reagent source for anti-A1?
dolichos biflorus
What is considered the definition of allogenic blood?
donated by a donor for a recipient other than the donor to use
An error was made in a handwritten blood bank record. Which of the following methods is acceptable for correcting the error to remain in compliance with current good manufacturing practices requirements?
draw a single line through the error so it can still be read and add initials next to it
A patient experiences a mild allergic reaction to a transfusion, including urticaria, skin redness, and itching. What is the most likely source of the allergen?
drugs or food consumed by the donor
The Rh nomenclature which uses the letters DCE is found in which of the following genetic models?
fisher-race
Which of these methods measures fetal hemoglobin or d positive red cells or both to evaluate fetomaternal hemorrhage?
flow cytometry
What sugar configuration is necessary as a base for attachment of other sugars to produce ABO antigens?
galactose plus fucose
When testing for the quality of platelet concentrates, all of the following must be included except
gas production
Many rbc processes, such as binding, transporting, and releasing oxygen, do not require energy. Energy however, is required for cellular viability. How does the rbc obtain most of its energy?
glycolytic pathways
Which additional information is required on a label or tie tag of an autologous unit?
id of patient
All of the following can be indications of anemia and the potential need for a transfusion of packed red cells except?
increased haptoglobin
Which of the following is generally considered equivalent to CMV seronegative RBC for use in an exchange transfusion of a newborn?
leukoreduced RBC
Acute transfusion reactions are divided into categories based on all of the following symptoms except:
liver faliure
A newborn presents with neonatal alloimmune thrombocytopenia. What is the most likely cause of this condition
maternal antibodies attacking neonatal platelet antigens
In HDFN, which of the following antigen-antibody reactions is occurring?
maternal antibody directed against fetal antigen
Which of the following conditions would not allow HDFN to occur as a result of Rh incompatibility?
mother and father both Rh neg
A prego female had RHIG at 28 weeks and has a positive antibody screen at delivery. The antibody has been confirmed as anti-D alone and reacts only weakly. This antibody may be the result of
passive anti-D(RhIg) received at 28 weeks
A patient front-grouped as AB but backed-grouped as O. the screening cells and auto control were positive. If the testing was performed correctly, what is the most likely cause of this discrepancy?
patient has a cold autoantibody
What should be done first if a donor unit of red blood cells is found to be incompatible at the AHG phase but compatible at IS with several different recipients?
perform a DAT on donor unit
Which procedure should be followed when one cross-matched unit out of five is incompatible at AHG phase?
perform a DAT on the incompatible unit