Ch 15

A geneticist introduces a transgene into yeast cells and isolates five independent cell lines in which the transgene has integrated into the yeast genome. In four of the lines, the transgene is expressed strongly, but in the fifth there is no expression at all. Which of the following is a likely explanation for the lack of transgene expression in the fifth cell line? A) The transgene integrated into a heterochromatic region of the genome. B) The transgene integrated into a euchromatic region of the genome. C) The transgene was mutated during the process of integration into the host cell genome. D) The host cell lacks the enzymes necessary to express the transgene. E) The transgene integrated into a region of the genome characterized by high histone acetylation.

A

A mutation that inactivates the regulatory gene of a repressible operon in an E. coli cell would result in A) continuous transcription of the structural gene controlled by that regulator. B) complete inhibition of transcription of the structural gene controlled by that regulator. C) irreversible binding of the repressor to the operator. D) inactivation of RNA polymerase by alteration of its active site. E) continuous translation of the mRNA because of alteration of its structure.

A

Most repressor proteins are allosteric. Which of the following binds with the repressor to alter its conformation? A) inducer B) promoter C) RNA polymerase D) transcription factor E) cAMP

A

The functioning of enhancers is an example of A) transcriptional control of gene expression. B) a post-transcriptional mechanism to regulate mRNA. C) the stimulation of translation by initiation factors. D) post-translational control that activates certain proteins. E) a eukaryotic equivalent of prokaryotic promoter functioning.

A

The phenomenon in which RNA molecules in a cell are destroyed if they have a sequence complementary to an introduced double-stranded RNA is called A) RNA interference. B) RNA obstruction. C) RNA blocking. D) RNA targeting. E) RNA disposal.

A

Which of the following best describes siRNA? A) a short double-stranded RNA, one of whose strands can complement and inactivate a sequence of mRNA B) a single-stranded RNA that can, where it has internal complementary base pairs, fold into cloverleaf patterns C) a double-stranded RNA that is formed by cleavage of hairpin loops in a larger precursor D) a portion of rRNA that allows it to bind to several ribosomal proteins in forming large or small subunits E) a molecule, known as Dicer, that can degrade other mRNA sequences

A

Which of the following is most likely to have a small protein called ubiquitin attached to it? A) a cyclin that usually acts in G1, now that the cell is in G2 B) a cell surface protein that requires transport from the ER C) an mRNA that is leaving the nucleus to be translated D) a regulatory protein that requires sugar residues to be attached E) an mRNA produced by an egg cell that will be retained until after fertilization

A

Which of the following mechanisms is used to coordinate the expression of multiple, related genes in eukaryotic cells? A) A specific combination of control elements in each gene's enhancer coordinates the simultaneous activation of the genes. B) The genes share a single common enhancer, which allows appropriate activators to turn on their transcription at the same time. C) The genes are organized into large operons, allowing them to be transcribed as a single unit. D) A single repressor is able to turn off several related genes. E) Environmental signals enter the cell and bind directly to promoters.

A

A geneticist introduces a transgene into yeast cells and isolates five independent cell lines in which the transgene has integrated into the yeast genome. In four of the lines, the transgene is expressed strongly, but in the fifth there is no expression at all. Of the lines that express the transgene, one is transcribed but not translated. Which of the following is a likely explanation? A) no promoter B) no AUG in any frame C) no compatible ribosome D) high histone acetylation E) missing transcription factor

B

A researcher found a method she could use to manipulate and quantify phosphorylation and methylation in embryonic cells in culture. In one set of experiments using this procedure in Drosophila, she was readily successful in increasing phosphorylation of amino acids adjacent to methylated amino acids in histone tails. Which of the following results would she most likely see? A) increased chromatin condensation B) decreased chromatin condensation C) activation of histone tails for enzymatic function D) decreased binding of transcription factors E) inactivation of the selected genes

B

Two potential devices that eukaryotic cells use to regulate transcription are A) DNA methylation and histone amplification. B) DNA amplification and histone methylation. C) DNA acetylation and methylation. D) DNA methylation and histone modification. E) histone amplification and DNA acetylation.

D

A researcher found a method she could use to manipulate and quantify phosphorylation and methylation in embryonic cells in culture. In one set of experiments she succeeded in decreasing methylation of histone tails. Which of the following results would she most likely see? A) increased chromatin condensation B) decreased chromatin condensation C) activation of histone tails for enzymatic function D) decreased binding of transcription factors E) inactivation of the selected genes

B

Allolactose, an isomer of lactose, is formed in small amounts from lactose. An E. coli cell is presented for the first time with the sugar lactose (containing allolactose) as a potential food source. Which of the following occurs when the lactose enters the cell? A) The repressor protein attaches to the regulator. B) Allolactose binds to the repressor protein. C) Allolactose binds to the regulator gene. D) The repressor protein and allolactose bind to RNA polymerase. E) RNA polymerase attaches to the regulator.

B

Among the newly discovered small noncoding RNAs, one type reestablishes methylation patterns during gamete formation and blocks expression of some transposons. These are known as A) miRNA. B) piRNA. C) snRNA. D) siRNA. E) RNAi.

B

In eukaryotes, general transcription factors A) are required for the expression of specific protein-encoding genes. B) bind to other proteins or to a sequence element within the promoter called the TATA box. C) inhibit RNA polymerase binding to the promoter and begin transcribing. D) usually lead to a high level of transcription even without additional specific transcription factors. E) bind to sequences just after the start site of transcription.

B

In positive control of several sugar-metabolism-related operons, the catabolite activator protein (CAP) binds to DNA to stimulate transcription. What causes an increase in CAP? A) increase in glucose and increase in cAMP B) decrease in glucose and increase in cAMP C) increase in glucose and decrease in cAMP D) decrease in glucose and increase in repressor E) decrease in glucose and decrease in repressor

B

In response to chemical signals, prokaryotes can do which of the following? A) turn off translation of their mRNA B) alter the level of production of various enzymes C) increase the number and responsiveness of their ribosomes D) inactivate their mRNA molecules E) alter the sequence of amino acids in certain proteins

B

Transcription of the structural genes in an inducible operon A) occurs continuously in the cell. B) starts when the pathway's substrate is present. C) starts when the pathway's product is present. D) stops when the pathway's product is present. E) does not result in the production of enzymes.

B

When DNA is compacted by histones into 10-nm and 30-nm fibers, the DNA is unable to interact with proteins required for gene expression. Therefore, to allow for these proteins to act, the chromatin must constantly alter its structure. Which processes contribute to this dynamic activity? A) DNA supercoiling at or around H1 B) methylation and phosphorylation of histone tails C) hydrolysis of DNA molecules where they are wrapped around the nucleosome core D) accessibility of heterochromatin to phosphorylating enzymes E) nucleotide excision and reconstruction

B

Which of the following would not be true of cDNA produced using human brain tissue as the starting material? A) It could be amplified by the polymerase chain reaction. B) It would contain sequences representing all the genes in the genome. C) It was produced from mRNA using reverse transcriptase. D) It could be used as a probe to detect genes expressed in the brain. E) It lacks the introns of the human genes.

B

Which of the following, when taken up by the cell, binds to the repressor so that the repressor no longer binds to the operator? A) ubiquitin B) inducer C) promoter D) repressor E) corepressor

B

Which one of the following techniques involves reverse transcriptase, PCR amplification, and gel electrophoresis? A) DNA microarray assays B) RT-PCR C) in situ hybridization D) RNA interference E) nucleic acid hybridization

B

You are given an experimental problem involving control of a gene's expression in the embryo of a particular species. One of your first questions is whether the gene's expression is controlled at the level of transcription or translation. Which of the following might best give you an answer? A) You explore whether there has been alternative splicing by examining amino acid sequences of very similar proteins. B) You measure the quantity of the appropriate pre-mRNA in various cell types and find they are all the same. C) You assess the position and sequence of the promoter and enhancer for this gene. D) An analysis of amino acid production by the cell shows you that there is an increase at this stage of embryonic life. E) You use an antibiotic known to prevent translation.

B

A researcher introduces double-stranded RNA into a culture of mammalian cells, and can identify its location or that of its smaller subsections experimentally, using a fluorescent probe. In addition, she finds what other evidence of this single-stranded RNA piece's activity? A) She can measure the degradation rate of the remaining single strand. B) She can measure the decrease in the concentration of Dicer. C) The rate of accumulation of the polypeptide encoded by the target mRNA is reduced. D) The amount of miRNA is multiplied by its replication. E) The cell's translation ability is entirely shut down.

C

Altering patterns of gene expression in prokaryotes would most likely serve the organism's survival in which of the following ways? A) organizing gene expression so that genes are expressed in a given order B) allowing each gene to be expressed an equal number of times C) allowing the organism to adjust to changes in environmental conditions D) allowing young organisms to respond differently from more mature organisms E) allowing environmental changes to alter the prokaryote's genome

C

DNA microarrays have had a huge impact on genomic studies because they A) can identify the function of any gene in a genome. B) can be used to introduce entire genomes into bacterial cells. C) allow the expression of many or even all of the genes in a genome to be compared at once. D) allow physical maps of the genome to be assembled in a very short time. E) dramatically enhance the efficiency of restriction enzymes (endonucleases).

C

During DNA replication, A) all methylation of the DNA is lost at the first round of replication. B) DNA polymerase is blocked by methyl groups, and methylated regions of the genome are therefore left uncopied. C) methylation of the DNA is maintained because methylation enzymes act at DNA sites where one strand is already methylated and thus correctly methylates daughter strands after replication. D) methylation of the DNA is maintained because DNA polymerase directly incorporates methylated nucleotides into the new strand opposite any methylated nucleotides in the template. E) methylated DNA is copied in the cytoplasm, and unmethylated DNA is copied in the nucleus.

C

For a repressible operon to be transcribed, which of the following must occur? A) A corepressor must be present. B) RNA polymerase and the active repressor must be present. C) RNA polymerase must bind to the promoter, and the repressor must be inactive. D) RNA polymerase cannot be present, and the repressor must be inactive. E) RNA polymerase must not occupy the promoter, and the repressor must be inactive.

C

Gene expression might be altered at the level of post-transcriptional processing in eukaryotes rather than prokaryotes because of which of the following? A) Eukaryotic mRNAs get 5' caps and 3' tails. B) Prokaryotic genes are expressed as mRNA, which is more stable in the cell. C) Eukaryotic exons may be spliced in alternative patterns. D) Prokaryotes use ribosomes of different structure and size. E) Eukaryotic coded polypeptides often require cleaving of signal sequences before localization.

C

If you were to observe the activity of methylated DNA, you would expect it to A) be replicating nearly continuously. B) be unwinding in preparation for protein synthesis. C) have turned off or slowed down the process of transcription. D) be very actively transcribed and translated. E) induce protein synthesis by not allowing repressors to bind to it.

C

One way scientists hope to use the recent knowledge gained about noncoding RNAs lies with the possibilities for their use in medicine. Of the following scenarios for future research, which would you expect to gain most from RNAs? A) exploring a way to turn on the expression of pseudogenes B) targeting siRNAs to disable the expression of an allele associated with autosomal recessive disease C) targeting siRNAs to disable the expression of an allele associated with autosomal dominant disease D) creating knock-out organisms that can be useful for pharmaceutical drug design E) looking for a way to prevent viral DNA from causing infection in humans

C

Researchers are looking for better treatments for breast cancer. For a particular DNA microarray assay (DNA chip), cDNA has been made from the mRNAs of a dozen patients' breast tumor biopsies. The researchers will be looking for a A) particular gene that is amplified in all or most of the patient samples. B) pattern of fluorescence that indicates which cells are overproliferating. C) pattern shared among some or all of the samples that indicates gene expression differing from control samples. D) group of cDNAs that act differently from those on the rest of the grid. E) group of cDNAs that match those in non-breast-cancer control samples from the same population.

C

There is a mutation in the repressor that results in a molecule known as a super-repressor because it represses the lac operon permanently. Which of these would characterize such a mutant? A) It cannot bind to the operator. B) It cannot make a functional repressor. C) It cannot bind to the inducer. D) It makes molecules that bind to one another. E) It makes a repressor that binds CAP.

C

Which of the following is an example of post-transcriptional control of gene expression? A) the addition of methyl groups to cytosine bases of DNA B) the binding of transcription factors to a promoter C) the removal of introns and alternative splicing of exons D) the binding of RNA polymerase to transcription factors E) the folding of DNA to form heterochromatin

C

At the beginning of this century there was a general announcement regarding the sequencing of the human genome and the genomes of many other multicellular eukaryotes. There was surprise expressed by many that the number of protein-coding sequences was much smaller than they had expected. Which of the following could account for most of the rest? A) "junk" DNA that serves no possible purpose B) rRNA and tRNA coding sequences C) DNA that is translated directly without being transcribed D) non-protein-coding DNA that is transcribed into several kinds of small RNAs with biological function E) non-protein-coding DNA that is transcribed into several kinds of small RNAs without biological function

D

Genomic imprinting, DNA methylation, and histone acetylation are all examples of A) genetic mutation. B) chromosomal rearrangements. C) karyotypes. D) epigenetic phenomena. E) translocation.

D

If a particular operon encodes enzymes for making an essential amino acid and is regulated like the trp operon, then A) the amino acid inactivates the repressor. B) the enzymes produced are called inducible enzymes. C) the repressor is active in the absence of the amino acid. D) the amino acid acts as a corepressor. E) the amino acid turns on transcription of the operon.

D

In a genome-wide expression study using a DNA microarray assay, each well is used to detect the A) fate of proteins produced by a cell. B) location of a protein produced by a cell. C) location of a gene within a cell. D) expression of a specific gene by a cell. E) type of chemical modification of proteins produced by a cell.

D

Steroid hormones produce their effects in cells by A) activating key enzymes in metabolic pathways. B) activating translation of certain mRNAs. C) promoting the degradation of specific mRNAs. D) binding to intracellular receptors and promoting transcription of specific genes. E) promoting the formation of looped domains in certain regions of DNA.

D

Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. If she moves the operator to the far end of the operon, past the transacetylase (lacA) gene, which of the following would likely occur when the cell is exposed to lactose? A) The inducer will no longer bind to the repressor. B) The repressor will no longer bind to the operator. C) The operon will never be transcribed. D) The structural genes will be transcribed continuously. E) The repressor protein will no longer be produced.

D

Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. If she moves the promoter for the lac operon to the region between the beta galactosidase (lacZ) gene and the permease (lacY) gene, which of the following would be likely? A) The three structural genes will be expressed normally. B) RNA polymerase will no longer transcribe permease. C) The operon will no longer be inducible. D) Beta galactosidase will not be produced. E) The cell will continue to metabolize but more slowly.

D

The lactose operon is likely to be transcribed when A) there is more glucose in the cell than lactose. B) the cyclic AMP levels are low. C) there is glucose but no lactose in the cell. D) the cyclic AMP and lactose levels are both high within the cell. E) the cAMP level is high and the lactose level is low.

D

Transcription factors in eukaryotes usually have DNA binding domains as well as other domains that are also specific for binding. In general, which of the following would you expect many of them to be able to bind? A) repressors B) ATP C) protein-based hormones D) other transcription factors E) tRNA

D

What would occur if the repressor of an inducible operon were mutated so it could not bind the operator? A) irreversible binding of the repressor to the promoter B) reduced transcription of the operon's genes C) buildup of a substrate for the pathway controlled by the operon D) continuous transcription of the operon's genes E) overproduction of catabolite activator protein (CAP)

D

Which of the following is a protein produced by a regulatory gene? A) operon B) inducer C) promoter D) repressor E) corepressor

D

A lack of which molecule would result in the cell's inability to "turn off" genes? A) operon B) inducer C) promoter D) ubiquitin E) corepressor

E

A researcher found a method she could use to manipulate and quantify phosphorylation and methylation in embryonic cells in culture. One of her colleagues suggested she try increased methylation of C nucleotides in a mammalian system. Which of the following results would she most likely see? A) increased chromatin condensation B) decreased chromatin condensation C) activation of histone tails for enzymatic function D) decreased binding of transcription factors E) inactivation of the selected genes

E

Since Watson and Crick described DNA in 1953, which of the following might best explain why the function of small RNAs is still being explained? A) As RNAs have evolved since that time, they have taken on new functions. B) Watson and Crick described DNA but did not predict any function for RNA. C) The functions of small RNAs could not be approached until the entire human genome was sequenced. D) Ethical considerations prevented scientists from exploring this material until recently. E) Changes in technology as well as our ability to determine how much of the DNA is expressed have now made this possible.

E

Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. If she moves the repressor gene (lac I), along with its promoter, to a position some several thousand base pairs away from its normal position, which will you expect to occur? A) The repressor will no longer be made. B) The repressor will no longer bind to the operator. C) The repressor will no longer bind to the inducer. D) The lac operon will be expressed continuously. E) The lac operon will function normally.

E

The role of a metabolite that controls a repressible operon is to A) bind to the promoter region and decrease the affinity of RNA polymerase for the promoter. B) bind to the operator region and block the attachment of RNA polymerase to the promoter. C) increase the production of inactive repressor proteins. D) bind to the repressor protein and inactivate it. E) bind to the repressor protein and activate it.

E

The tryptophan operon is a repressible operon that is A) permanently turned on. B) turned on only when tryptophan is present in the growth medium. C) turned off only when glucose is present in the growth medium. D) turned on only when glucose is present in the growth medium. E) turned off whenever tryptophan is added to the growth medium.

E

Which of the following statements about the DNA in one of your brain cells is true? A) Most of the DNA codes for protein. B) The majority of genes are likely to be transcribed. C) Each gene lies immediately adjacent to an enhancer. D) Many genes are grouped into operon-like clusters. E) It is the same as the DNA in one of your kidney cells.

E