CH 15 test bank

Which of the following is a region in DNA that binds to a steroid receptor?

A)GRE

Which of the following would also work as an enhancer for the following bi directional enhancer? 5' GTTC 3' 3' CAAG 5'

A. 5' GAAC 3' 3' CTTG 5'

12. CpG islands are associated with which of the following?

B)DNA methylation

13. Genomic imprinting is a result of ___________.

DNA methylation

8. Which of the following recognizes response elements, control elements, and regulatory elements?

Regulatory transcription factors

. What basal transcription factor is most often affected by regulatory transcription factors?

TFIID`

The activity of some transcription factors can be regulated by covalent modifications.

TRUE

Transcription factors are proteins that influence the ability of the RNA polymerase to transcribe a gene.

TRUE

5. What activates CREB?

phsophorylation

which of the following structural motifs promotes dimerization?

B)leucine zipper

Which of the following might complicate the interpretation of ChIP-Seq data?

Because histones can be moved around or ejected from the chromatin, some DNA might not be sequenced if it is not wrapped around a histone

Which of the following is the most likely location of an insulator sequence?

Between two genes

Histone acetyltransferases are directly involved in which of the following?

C)Chemical modifaction of histones

6. What would be the result of a mutation in Hsp90?

C)expression of the regulated genes would become constitutive

10. Which one of the following directly interacts with the DNA as a transcriptional regulator?

CREB protein dimer

Which DNA sequence important for gene expression is usually contained within the NFR?

Core promoter

Which of the following is a major finding from the ENCODE project?

DNA methylation usually signifies the activation of gene expression.

. A mutation in which of the following would result in little or no effect on the expression of a gene regulated by a CRE?

F) Glucocorticoid receptor

. Activator proteins bind to silencer sequences and repressor proteins bind to enhancer sequences.

FALSE

A heterodimer occurs when two identical transcription factors interact on a sequence of DNA.

FALSE

A repressor protein would enhance the ability of TFIID to bind to the TATA box of the promoter.

False

A transactivation domain is an example of a motif found in transcription factors.

False

DNA methylation usually activates gene expression.

False

DNA that contains actively transcribed genes would most likely contain chromatin in the closed configuration

False

Insulators exert their effects on gene expression through histone acetyltransferase, histone deacetylase, and RNA polymerase.

False

Methylation of DNA bases is one way that regulatory transcription factors may influence gene expression.

False

Prior to hormone binding, receptors for steroid hormones are usually found in the nucleus of the cell.

False

The exons of a gene are always expressed in a functional protein.

False

For which of the following histones has variant forms not yet been identified?

H4

In ChIP-Seq, the DNA fragments to be sequenced are around 150 bp in length. What does this size of DNA represent?

The amount of DNA wrapped around a nucleosome

A particular cell contains all of the standard histones but lacks several histone variants. Which of the following MAY be true of this cell?

The cell will express different sets of genes than other cells in the same organism.

A particular gene has a mutation in its NFR that causes it to bind abnormally tightly to histones. What effect would you expect this mutation has on the expression of this gene?

The expression of the gene would be abnormally low or absent.

What might happen if the insulator between two genes is missing?

Transcription factors influencing one gene's expression may abnormally influence a neighboring gene's expression.

Housekeeping genes are unmethylated and active in most cells.

True

If a portion of a transcription factor's domain is the same in a variety of organisms, it is called a motif

True

Nucleosome location may be changed by a process called ATP-dependent chromatin remodeling.

True

Steroid hormomes are an example of an effector which regulates regulatory transcription factor activity.

True

When cortisol binds to the glucocorticoid receptor, HSP90 is released.

True

cAMP is known as a second messenger since the pathway is first activated by a extracellular signaling molecule.

True