Forensic Analysis/Restriction enzymes
Why must DNA be digested before gel electrophoresis?
DNA must be digested before gel electrophoresis to break the DNA into fragments to produce bands
What is the difference between qualitative and quantitative measurements?
Qualitative is data that is not numerical. Quantitative is measurement of data that can be put into numbers
What is the purpose of conducting a gel electrophoresis?
The purpose of gel electrophoresis is to find and compare DNA sequences to determine alterations or find matches.
What is the purpose of the agarose gel in gel electrophoresis?
The purpose of the aragose gel is so that the DNA has a platform to cross and organize DNA.
What is the function of the electric current?
The purpose of the current is to push the negatively charged DNA through the gel
Which bones proved most useful in determining sex? race? height?
sex: pelvis, skull, femur, tibia, humerus race: skull height: femur, tibia, humerus
Why is it useful to use more than one restriction enzyme during DNA fingerprinting?
so you get more accurate results
What would happen if a student group put their DNA in the electrophoresis box incorrectly? With the wells containing DNA oriented toward the positive end?
They would not move because "opposites attract" and DNA is negatively charged so it would not move to the negatively charged end.
What does the term "sticky end" refer in the context of restriction enzymes?
The ends of the cut have an overhanging piece of single-stranded DNA are called "sticky ends" because they are able to form base pairs with any DNA molecule that contains the complementary sticky end.
In what organisms dis restriction enzymes evolve? What was their purpose in that organism?
they evolve from bacteria. Their purpose was to protect the body/bacteria from virus DNA.
What is the function of a restriction enzyme?
they recognize specific sequences in DNA and then cut the DNA and then cut the DNA to produce fragments, called restriction fragments.