Gel Electrophoresis Steps
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Step 3
A current is applied to the gel. DNA has a negative charge
Step 2
All pieces are placed into small wells in the agar (in a buffer solution)
Step 1
DNA is cut into varying sized pieces with restriction enzymes
Step 5
DNA is stained and banding pattern is observed
Gel Electrophoresis
Separating different sized pieces of DNA through agarose gel by applying an electric current
Step 4
The length the DNA moves is inversely proportional to the size of the fragment (smaller move farther)