Kato Thick & Kato-Katz Technique

Steps 1-3

- procedure on how to prepare your Kato Thick Smear Technique

Step 4

- reporting and grading of results

Kato-katz technique

-The procedure is useful for assessing the intensity of infection with Schistosoma and common soil-transmitted helminths like Ascaris, Trichuris, and hookworm. -QUANTITATIVE TECHNIQUE FOR COUNTING HELMINTH EGG

50 and 60

CELLOPHANE THICK SMEAR -About __ to __ mg of stool (approximately the size of two mung beans) is placed over a glass slide and covered with cut cellophane paper soaked in a mixture of glycerine and malachite green solution.

10 and 20

CELLOPHANE THICK SMEAR -The preparation is best examined within __ to __ minutes

Glycerine

CELLOPHANE THICK SMEAR -is a clearing solution

Malachite green

CELLOPHANE THICK SMEAR -is used to give color to the cellophane in order to give a pale green background to the eggs and to minimize the brightness of the microscopic field.

thick and thin

CELLOPHANE THICK SMEAR It is very good in detecting eggs with ______ shells (e.g. Ascaris and Trichuris) but not eggs with ______ shells (e.g. hookworm).

24

CELLOPHANE THICK SMEAR MATERIALS Cellophane cut into squares 1 x 1 are soaked in malachite green solution for __ hours before use.

Distilled water, glycerine, and 3% malachite green solution in water.

CELLOPHANE THICK SMEAR MATERIALS The formula for glycerine-malachite green solution is as follows: - _________ ______ ± 500ml - ________ ± 500ml - ___________ _________________ ± 5ml

Glycerine

CELLOPHANE THICK SMEAR MATERIALS in the mixture provides a clearer differentiation of the eggs.

Cellophane

CELLOPHANE THICK SMEAR MATERIALS protects the eyes from the strong light during microscopic exam.

Ascaris lumbricoides

Identify

Fertilized egg of ascaris lumbricoides

Identify

Hookworm

Identify

Schistosoma mansoni

Identify

Unfertilized egg of ascaris lumbricoides

Identify

hookworm

Identify

3% malachite green, glycerol, and distilled water

KATO-KATZ TECHNIQUE materials _ ______ _____ 1 ml _________100 ml _________100 ml

3, 4, and 1.37

Kato-katz technique materials -Template either made of plastic or cardboard (_ x_ x_.__ cm) with a 6 mm hole in its center.

Kato-katz technique

Purpose: To detect helminth egg in feces, including those soil-transmitted helminth and intestinal schistosoma

Kato thick and kato-katz technique

to determine if there are any helminth eggs or if there is intestinal infection of your helminths