MICROSCOPY midexam

Staining cells such that their color is different from the background enhances which quality of the cells?

Contrast

What is enhanced when a stain is used to make a sample, or parts of a sample, darker than the background in a slide?

Contrast

Which of the following is defined as the ability to distinguish an object from the back

Contrast

Which of the following is defined as the ability to distinguish an object from the background

Contrast

Which of these challenges are microscopes used to overcome?

Many biological specimens are too small to see with the unaided eye.

Which of these challenges are microscopes used to overcome? Multiple choice question.

Many biological specimens are too small to see with the unaided eye.

What is the goal of microscopy?

To create a magnified image of objects that are too small to be seen with the unaided eye

Which microscopy method is beneficial because of its applications for living specimens?

Wet mount

Resolution is an image quality factor that involves being able to see fine , --- which is essential for image clarity.

details

The function of immersion oil in microscopy is to

direct more light rays to the objective lens

Changing the magnification of an image with a microscope changes the portion of the slide that is visible, or the

field of view.

The portion of a slide that is visible through the eyepiece is called the

field of view.

All the organisms that can be seen through naked eyes are

macroscopic organisms

All the organisms that cannot be seen through naked eyes are

microscopic orgainsms

The total magnification of an image is determined by ______ the magnification power of the ocular lens and the magnification power of the objective lens used.

multiplying

The goal of microscopy is to create a magnified image of

objects that are too small to be seen with the unaided eye.

Increasing the _____ would allow you to tell if what appears as one object is really two objects extremely near each other.

resolution

The portion of a slide that is visible through the eyepiece is called the field of -----

views

Select the specimen that could be viewed using a light microscope.

-Bacteria -Cells -Tissues

Select the components used by a bright-field microscope to view a specimen.

-Glass lenses -A light source

Which metric units are most appropriate for expressing the size of macroscopic organisms?

-Meters -Centimeters

Select the benefits of wet mount sample preparation for microscopy.

-The preparation is relatively simple and fast. -Living organisms can be viewed. -Motility of specimens can be observed.

Fill in the blank question. A microscopy sample that is prepared by suspending it in a fluid that temporarily keeps it alive and gives it space to move is called a --- ----

-wet -mount

Which objective lens is used with a sample that has been prepared with a wet mount?

10× low-power lens

If a specimen is being viewed using a 20× objective lens and the ocular lenses, what is the total magnification?

200×

Which lens has a magnification of 40×?

High-power objective

Which phenomenon is reduced by oil immersion microscopy?

Loss of light that scatters into the air or onto the slide

If you are unable to see something with your eyes, you can increase the _____ to make it look bigger.

Magnification

Which image quality factor is defined as the ability to distinguish fine detail?

Resolution