Section 1

What is true about non-replicative transposons? A. Can physically leave a sequence and "hop" to another sequence. B. Can result in disrupting a gene. C. Can be present on plasmids. D. Cell carry antibiotic resistant genes. E. All statements are true.

E. All statements are true!

T/F: The trp operon is constitutively expressed

FALSE

What structure is NEVER found in Gram-positive bacteria?

LPS

Which of the following is NOT true regarding regulation of the Lac operon when Lactose and Glucose are present?

LacZ, which encodes B-galactosidase, is transcribed at high levels

Streptococcus is....

A Gram-positive cocci.

What is NOT true of the Sensor Kinase in a Two Component Signal Transduction System? A. binds the chromosome to alter transcription B. Is active when phosphorylated C. Is activated by environmental signals D. found on the cell surface E. all of the above are true

A. Binds the chromosome to alter transcription

The tryptophan operon has a leader sequence that can regulate transcription by what process?

Attenuation

If a bacterium cannot grow on a defined minimal media, it is called a(n) ________?

Autotroph

What is TRUE of Horizontal Gene transfer by transduction? A. uptake of naked DNA B. requires cell contact C. initiated by transposons D. initiated by bacteriophage E. is mediated by plasmids

D. initiated by bacteriophage

When performing fluorescent microscopy, if you want to visualize nucleic acid, which stain would you use?

DAPI

Which molecule is responsible for loading DNA helicase during replication?

DnaC

What is true about phage capsids? A. They can be filamentous B. They contain nucleic acid C. They are constructed of repeating protein units D. They can be icosohedral E. all of the above are true

E. all of the above are true

You isolate an unknown bacteria growing on MacConkey agar that contains lactose. You know just from looking at the colonies, which are white, that your unknown will likely be which of the following?

Gram-negative, does not ferment lactose

The genes in the Lac operon are considered to be?

Inducible

Bacteria that obtain carbon from organic compounds and derive energy from light are called?

Photoheterotroph

What cell surface structure is ONLY found on Gram-positive bacteria?

S-layer

What structure protects bacteria against harsh environment conditions?

SPORE

What is most unique to the life cycle of a lysogenic phage.

The phage DNA integrates into the bacterial chromosome

What type of microscopy would be the best to visualize bacterial internal structures?

Transmission Electron Microscopy

What is TRUE when tryptophan is present (high levels) in the bacterial cell?

Trp repressor is bound to operator

How is Glucose is transported?

Uses the PTS system

A prophage refers to what part of the bacteriophage life cycle?

Where the phage genome is integrated into the host chromosome.

What is true about the process of bacterial chemotaxis?

flagella are turning counter clockwise.

The cell wall found in most bacteria consists of what structure?

peptidoglycan

What name refers to having flagella attached all around the cell?

peritrichous

What is true about a facultative anaerobe?

prefers to grow with no oxygen

What is true about conjugation?

requires cell-to-cell contact.

Gene transfer that involves a lysogenic bacteriophage is known as?

specialized transduction.

Peptidoglycan consists of two joined amino sugars (NAM and NAG): during cell wall synthesis which enzymes are responsible for forming the cross links between amino acids?

transpeptidases

DnaB is responsible for which process during DNA replication?

unwinding the double stranded DNA helix.

What is TRUE of Horizontal Gene transfer by transformation?

uptake of naked DNA