Biology Lab Study Guide

Select all that apply Which of the following are true statements about an enzyme-catalyzed reaction? An enzyme reduces the activation energy required for a reaction. An enzyme speeds up the conversion of substrate to product. Every time a substrate is converted to a product, a bit of the enzyme is used up. Enzymes are not used up in the chemical reaction.

An enzyme reduces the activation energy required for a reaction. An enzyme speeds up the conversion of substrate to product. Enzymes are not used up in the chemical reaction.

Select all that apply Which of the following is true about enzyme activity and pH? At a pH level above or below optimum, conversion of substrate to product happens more slowly. At optimum ph level, an enzyme converts substrate to product very slowly. Enzymes working at their optimum pH level will be most active. An enzyme converts substrate to product most quickly at optimum pH.

At a pH level above or below optimum, conversion of substrate to product happens more slowly. Enzymes working at their optimum pH level will be most active. An enzyme converts substrate to product most quickly at optimum pH.

How will you test the specificity of catalase in this experiment? Combine water and catalase in a test tube. Combine water and hydrogen peroxide in a test tube.

Combine water and catalase in a test tube.

If catalase (enzyme) is present in excess in a reaction, how will reducing the amount of hydrogen peroxide (substrate) in the reaction mix affect the amount of oxygen and water (products) produced? Increase the amount of oxygen and water (products) produced. Not effect the amount of oxygen and water (products) produced. Decrease the amount of oxygen and water (products) produced.

Decrease the amount of oxygen and water (products) produced.

Select all that apply Which of the following statements about enzymes are true? Enzymes lower the energy of activation for the reaction that they catalyze. Enzymes bind their substrate but are not changed in the reaction. Enzymes are not consumed in the reaction they catalyze. Enzymes are only able to be used once before becoming unstable.

Enzymes lower the energy of activation for the reaction that they catalyze. Enzymes bind their substrate but are not changed in the reaction. Enzymes are not consumed in the reaction they catalyze.

Enzymes are inorganic molecules. True / False

False

To determine the activity of an enzyme, you can compare the concentration of the enzyme before and after the chemical reaction. True / False

False

When water is added to hydrogen peroxide in this experiment, you will expect to see oxygen bubbles. True / False

False

Select all that apply Hydrogen peroxide is broken down into oxygen and water with the help of the enzyme catalase. Which of the following can be measured to determine the activity of the enzyme catalase? How much hydrogen peroxide is consumed in the reaction. How much water is produced. How much oxygen is produced. How much catalase is consumed during the reaction.

How much hydrogen peroxide is consumed in the reaction. How much water is produced. How much oxygen is produced.

The pH of an enzyme solution can alter the shape of the enzyme and its active site by affecting ________ bonding.

Ionic / Chemical

Select all that apply Which of the following is/are method(s) used to measure the activity of an enzyme? Measuring how much substrate is used. Measuring the rate at which the temperature changes. Measuring how much enzyme is consumed. Measuring how much product is made.

Measuring how much substrate is used. Measuring how much product is made.

Select all that apply Heating up a solution of molecules will result in which of the following events to occur? Movement of molecules will stay the same. Molecules will move around faster. Molecules will bump into one another more frequently. Molecules will move more slowly.

Molecules will move around faster. Molecules will bump into one another more frequently.

Select all that apply If enough substrate is present, how will adding more enzyme to a reaction mixture affect the rate of product formation? More enzyme will speed up the rate of product formation. More enzyme will decrease the rate of product formation. More enzyme will slow down the rate of product formation. More enzyme will increase the rate of product formation.

More enzyme will speed up the rate of product formation. More enzyme will increase the rate of product formation.

If you add more catalase (enzyme) to an excess amount of hydrogen peroxide (substrate), what will happen to the products? Oxygen (product) will be produced faster Water (product) will be consumed faster Water (product) will be produced slower

Oxygen (product) will be produced faster

What do you predict will happen when you mix water with the enzyme catalase in this experiment? Oxygen bubbles will form Oxygen bubbles will not form Catalase will be broken down

Oxygen bubbles will not form

Measuring the amount of _______ made by a reaction is one way to determine the activity of an enzyme

Product

What is the term for the substance that is formed after a chemical reaction occurs? Product Substrate Reactant Enzyme

Product

What effect do enzymes have on the rate of a chemical reaction? No effect on chemical reactions Speed up chemical reactions Slow down chemical reactions

Speed up chemical reactions

What is another term for the reactant that combines with an enzyme in a reaction? Active site Product Substrate

Substrate

What substance decreases during a chemical reaction and can be used to measure the activity of an enzyme? Substrate Product Enzyme

Substrate

Select all that apply Why is enzyme concentration NOT used to measure the rate of the enzyme-catalyzed reaction? The concentration of an enzyme used in a chemical reaction does not change. It is too difficult to measure the concentration of an enzyme used in a chemical reaction. The enzyme is completely used up after a chemical reaction

The concentration of an enzyme used in a chemical reaction does not change.

In this experiment, how will you recognize when a chemical reaction between hydrogen peroxide and catalase is occurring? The consumption of hydrogen peroxide will decrease the contents of the test tube. The production of oxygen will cause bubbling in the test tube. The production of water will increase the volume of the test tube.

The production of oxygen will cause bubbling in the test tube.

Select all that apply What is the purpose of preparing a tube with water and hydrogen peroxide in this experiment? This tube acts as a positive control. This tube acts as a negative control.

This tube acts as a negative control.

At higher temperatures, an enzyme may denature. True / False

True

Catalase activity in the reaction can be detected by observing the formation of oxygen bubbles. True / False

True

Changing the pH of an enzyme reaction can alter how rapidly a product is formed. True / False

True

An enzyme reduces the energy necessary to start a chemical reaction. This means that the rate of the reaction will ___.

increase

Increasing the concentration of enzyme at the beginning of the experiment results in ___ substrate interacting with the enzyme per unit of time. less more the same amount of

more

Identify which statement(s) about enzymes is/are FALSE? Most enzymes are proteins. An enzyme binds to a substrate at the passive site. Enzymes increase the rate of chemical reactions. Enzymes can bind with many different substrates.

An enzyme binds to a substrate at the passive site. Enzymes can bind with many different substrates.