Ch 22 Linkage mapping
In situ Hybridization
(in place) procedure conducted on chromosomes that are held in place (adhered to a surface) - DNA probes hybridize to specific chromosomes (Color them)
What is a sequence-tagged site?
A DNA sequence that can be amplified uniquely by PCR
What is a microsatellite?
A short DNA sequence repeated many times in a row
A collection of recombinant vectors that each contain a particular fragment of chromosomal DNA is called a DNA
DNA Library
linkage studies
Frq of recombinant offspring.
A research endeavor with the goal of determining the sequence of DNA bases in an organism's entire genome is called a(n) - project.
Genome Sequencing Project
Physical mapping
Most specific/Highly detailed (uses cloned genes to define regions of chromosomes)
If DNA is collected from a haploid cell, how many bands will be produced by an STS that is amplified by PCR and subjected to gel electrophoresis?
One
Which mapping strategy involves cloning many pieces of DNA and characterizing their sizes and relative locations along a chromosome?
Physical mapping
To analyze a microsatellite sequence, a scientist must first amplify it by PCR. This requires the use of PCR ___________ that flank the repeated sequence in the microsatellite.
Primers (Must be complementary)
Mapping
Process of determining the relative locations of genes or other segments on a Chromosome.
Molecular Marker
Segment of DNA that is found at a specific site along a chromosome (vary from individual to individual)
Cytogenetic mapping
Viewed Microscopically, commonly used for eukayotes (due to them being larger in size) can be distinguished using size, centromere location, and banding patterns (dyes produce)
STS (sequence tagged sites)
any marker amplified by PCR
The presence of two bands corresponding to a sequence-tagged site that has been amplified by PCR and subjected to gel electrophoresis indicates that the ______.
cell was heterozygous for the STS
A short repetitive DNA sequence, such as a dinucleotide sequence repeated many times in a row, is called a(n) Blank______.
microsatellite
A microsatellite can be amplified by PCR using primers that are located ______ the microsatellite.
on both sides of
A DNA library is a collection of ______.
recombinant vectors containing various fragments of chromosomal DNA.
