chapter 3 labratory
What percentage of agar is typically added to nutrient broth to make nutrient agar? Excess of 25% 1% to 5% 15% to 25% 6% to 10%
1% to 5%
Phase-contrast microscopy compared to bright-field microscopy gives which of the following? Less internal detail of the cell Greater internal detail of the cell Only external cell detail About the same amount of cell structure detail
Greater internal detail of the cell
Which of the following is an organic compound, such as a vitamin or amino acid, that must be provided in the diet to facilitate survival of certain bacteria. Dye Mineral Glucose Growth factor
Growth factor
Which of the following methods would be preferred to visualize a microorganism for a true assessment of size, shape, color, and motility? Gram stain Basic stain Hanging drop method
Hanging drop method
The color-bearing portion of a dye is termed the _________ and has a charge that attracts it to certain cell parts bearing the opposite charge.
chromophore
The condenser converges (focuses) light to a single point on the specimen in a ________ microscope.
compound
A scanning __________ microscope uses a laser beam of light to scan various depths in a specimen.
confocal
Dyes can be used as differential agents because many of them are _________ indicators which change color in response to the production of acids or bases.
pH
To be effective, a microscope must have which of the following characteristics?(3) Multiple select question. UV fluorescence capabilities Adequate magnification Resolution Clarity of image
Adequate magnification Resolution Clarity of image
Identify the polysaccharide found in seaweed that is commonly used to prepare solid culture media. Gelatin Agar Peptone Starch
Agar
Which immunological compounds can be used in conjunction with fluorescent dyes to better visualize microorganisms? Neutrophils Antibodies T cells Complement proteins
Antibodies
How does a bacterial colony appear? Multiple choice question. Throughout liquid culture In a solid lawn of organisms on a solid agar plate As an individual isolate on solid agar
As an individual isolate on solid agar
Why is the use of immersion oil sometimes necessary when using a microscope? Because oil evaporates slower than water Because oil has the same refraction as glass and lens Because oil doesn't dissolve the specimen Because oil keeps the specimen wet
Because oil has the same refraction as glass and lens
Identify the selective agent in EMB agar. Vinegar Safranin Crystal violet Bile salts
Bile salts
Identify the selective agent in MacConkey agar that allows isolation of intestinal pathogens. Mucus Acid Bile salts Antibiotics
Bile salts
Which is a common component found in feces and, therefore, used in media to selectively culture Gram-negative bacteria? Bile salts Crystal violet Vinegar Malachite green
Bile salts
___________ ___________, or resolution defines the capacity of an optical system to distinguish tow adjacent objects or points from one another.
Blank 1: Resolving Blank 2: power
_______ power is the ability of a microscope to show detail.
Blank 1: Resolving or Resolution
_______ media are used to maintain and preserve specimens that have to be held for a period of time before clinical analysis.
Blank 1: Transport
The ________ ________ contrast microscope produces well-defined images that are vividly colored and appear three-dimensional.
Blank 1: differential Blank 2: interference
A _______ culture is a container that holds two or more easily differentiated species of microorganisms.
Blank 1: mixed or mix
Which of the following wavelengths should give the best resolution? Red Blue Yellow Green
Blue
How does a scanning electron microscope produce its image? Bombards the specimen with electrons while scanning it Bombards the specimen with a laser beam while scanning it Bombards the specimen with metal ions while scanning it
Bombards the specimen with electrons while scanning it
What is the total magnification for a microscope when the ocular is a 10X and you are using the high power objective 40X? Multiple choice question. 4X 50X 40X 4000X 400X
400x
Once liquefied, agar does not resolidify until it cools to what temperature? 60οC 75οC 42οC 100οC
42οC
Media that allows the growth of multiple types of microbes but are designed to distinguish characteristics among members of the culture are called which of the following? Selective media Special media Differential media Specific media
Differential media
Which of the following is a medium that works by showing two reaction types, such as a color change in some colonies but not in others? Minimal medium Enriched medium Selective medium Differential medium
Differential medium
True or false: A wet, hanging drop mount can assess whether an organism is Gram-negative or positive.
False
True or false: All specimens are prepared in the same manner for microscopic examination.
False
True or false: Mannitol Salt Agar (MSA) is typically used to grow Gram-negative bacteria.
False
Which staining is based on the dying of external cell structures used for locomotion in order to enlarge them and make them visible? Negative Flagellar Capsular Gram
Flagellar
Which of the following is an optical microscope that uses UV radiation as the illuminating source? Dark-field Fluorescence Interference Phase-contrast
Fluorescence
Capsule staining is used to observe the unstructured protective layer surrounding some of which groups of microorganisms? Multiple select question. Helminths Fungi Viruses Bacteria
Fungi Bacteria
Which of the following determines the type of microbe at the species level? Infection Incubation Inspection Isolation Identification
Identification
What is the name given to the circular plastic or glass dish that is used for plating in the loop dilution technique? Blunt Petri Sergei Well
Petri
Gram positive bacteria exhibit with color after the Gram-stain? Blue Pink Purple Green
Purple
Which of the following is defined as the magnification of the ocular lens times the magnification of the objective lens? Total magnification Resolution Index of refraction Resolving power
Total magnification
True or false: Liquid media can be used to better understand the oxygen demands of a microorganism.
True
Identify all the benefits of using solid agar compared to using liquid media.. Multiple select question.(3) Useful for isolating bacteria Useful for isolating fungi Provides a firm surface on which cells can form discrete colonies Solid agar allows greater growth of microorganisms.
Useful for isolating bacteria Useful for isolating fungi Provides a firm surface on which cells can form discrete colonies
The technique of subculturing bacteria is used to obtain pure or ______ cultures.
axenic
Negative staining of a cell can yield results concerning which of the following? Multiple select question.(3) Cell shape and arrangement The presence of a capsule Cell size The presence of flagella The type of cell wall
Cell shape and arrangement The presence of a capsule Cell size
Which of the following genera are capable of producing endospores? Multiple select question.(2) Streptococcus Escherichia Clostridium Staphylococcus Bacillus
Clostridium Bacillus
Which of the following microscope components is used to converge (or focus) the rays of light to a single point on the specimen? Iris diaphragm Condenser Stage Coarse adjustment knob Lamp
Condenser
Which of the following terms is used to describe a collection of bacteria growing in broth or on agar? Fastidious Growth Colony Culture
Culture
________ stains require only a single dye.
Simple
The numerical aperture in the oil immersion lens is approximately which of the following? 0.50 1.25 0.75 1.00
1.25
What concentration of gelatin should be added to broth to make a solid medium for bacterial growth? 20-25% 10-15% 1.0-1.5% Greater than 25%
10-15%
If the ocular is a 10X lens and the nosepiece objective happens to be a 10X objective, then what is the total magnification? 100X 1000x 20X 10X 1X
100X
Agar melts at which temperature? 75°C 100°C 42°C 80°C
100°C
Resolution is the ability of a microscope to do which of the following? Magnify an image large enough to view with the naked eye Accurately distinguish between two separate entities Use electrons to provide clarity of a very small organism Provide a real image to the eye in the ocular lens
Accurately distinguish between two separate entities
Which of the following staining procedures is used to detect Mycobacterium tuberculosis? Endospore stain Gram stain Negative stain Acid-fast stain
Acid-fast stain
Nonliquefiable solid media can contain one of the following materials as the solidifying agent except which of the following? Cooked meat media Potato slices Rice grains Agar
Agar
What is involved in making a smear? Multiple select question. Applying a liquid suspension to a cover slip Staining the slide background and then applying cells Air drying a liquid application on a glass slide Spreading a thin film of suspended cells on a glass slide
Air drying a liquid application on a glass slide Spreading a thin film of suspended cells on a glass slide
Which chemicals are used to fix microbial cells? Multiple select question.(2) Water Chloride Sodium Alcohol Formalin
Alcohol Formalin
Which of the following describes any technique which limits the possible contamination of media or self with unwanted microorganisms? Streak plate Aseptic Isolation Inoculation Culturing
Aseptic
Which are correct regarding the six "I"s used for culturing microorganisms? Multiple select question. (2) The six "I"s always begin with inoculation. Aseptic technique should be maintained. It is important to work with a pure culture for proper identification. The process is a progression of steps that must be followed in order.
Aseptic technique should be maintained. It is important to work with a pure culture for proper identification.
________ is a solidifying agent used in media that melts at 100°C and does not resolidify until it reaches 42°C.
Blank 1: Agar
_______ staining is designed to observe the unstructured protective layer surrounding some bacteria and fungi. Listen to the complete question
Blank 1: Capsule, Negative, or Capsular
__________ dyes have a positive charge, whereas _________ dyes have a negative charge.
Blank 1: Cationic or Basic Blank 2: Anionic or Acidic
___________ - ___________ microscopy is most effectively used to visualize living cells that would be distorted by drying or heat.
Blank 1: Dark Blank 2: field
________ staining is designed to distinguish between spores stained with malachite green and the vegetative cells from which they are produced.
Blank 1: Endospore or Spore
_______ staining is a method of revealing flagella.
Blank 1: Flagellar, Flagellum, or Structural
________ is the ability of a microscope to make objects appear enlarged.
Blank 1: Magnification
_________ staining is a general type of staining that does not disrupt the cell structure with heat fixation and can indicate the presence of a capsule.
Blank 1: Negative
________ solid media do not melt and contain materials such as rice grains, cooked meat media, and potato slices.
Blank 1: Nonliquefiable or Nonliquifiable
_______ media are useful for growing anaerobic bacteria.
Blank 1: Reducing
The selective agent in MacConkey Agar is _____ salts.
Blank 1: bile
Different types of culture ________ provide nutrients for microbial growth.
Blank 1: medium or media
The _______ ________ is the mathematical constant that describes the relative efficiency of a lens in bending light rays.
Blank 1: numerical Blank 2: aperture
Microscopes are equipped with a nosepiece holding three ________ lenses which vary in magnification.
Blank 1: objective
Basic dyes carry a _______ charged chrompohore and acidic dyes have a ________ charged chrompohore.
Blank 1: positively or positive Blank 2: negatively or negative
A _______ medium contains a substance such as thioglycollic acid which reduces the availability of oxygen in the medium.
Blank 1: reducing or anaerobic
The first microscopes were _______, meaning they contained just one magnifying lens and few working parts.
Blank 1: simple
A _________ -condenser is a small opaque disk that blocks all of the light from hitting the specimen directly and forces the light to come only from the sides of the specimen.
Blank 1: stop
Another name for chemically defined media is called _______ media.
Blank 1: synthetic
Stuart's and Amie's _______ media contain buffers and absorbants to prevent cell destruction but will not support growth.
Blank 1: transport
VBNC is the abbreviation for __________ but ________ and may describe most of the microbes in the environment.
Blank 1: viable Blank 2: nonculturable
Which type of microscope forms its image when light is transmitted through a specimen? Dark-field microscope Bright-field microscope Phase-contrast microscope
Bright-field microscope
How is semisolid media inoculated? By injecting with a syringe By stabbing carefully in the center By mixing the media By streaking on the surface
By stabbing carefully in the center
How does a transmission electron microscope produce its image? By transmitting light through the specimen By transmitting electrons through the specimen By transmitting a laser beam through the specimen
By transmitting electrons through the specimen
Which of the following are characteristics of an enriched medium? Multiple select question.(3) Can be used to aid in the growth of fastidious organisms May contain organic substances such as blood, serum or hemoglobin May contain special growth factors Contains antibiotics to assist in keeping cultures pure Its components are always chemically defined
Can be used to aid in the growth of fastidious organisms May contain organic substances such as blood, serum or hemoglobin May contain special growth factors
How much resolution can an electron microscope obtain (i.e. what can it see)? Can see structures as small as 0.5 mm Can see structures as small as 0.5 nm Can see structures as small as 100 nm Dark-field microscopes can magnify better.
Can see structures as small as 0.5 nm
All of the following apply to the loop dilution and pour plate method except which one? Multiple choice question. Colonies grow on the surface and within the medium. The number of cells is reduced in each liquid agar tube. Cells have ample space to grow into discrete colonies. Colonies grow only on the surface of the plates.
Colonies grow only on the surface of the plates.
Which term is used to describe a cluster of visible bacteria of the same species on an agar plate? Lawn Mold Colony Plaque
Colony
Which of the following is the defining difference between compound microscopes and simple microscopes? Compound microscopes have an "arm". Compound microscopes have an iris diaphragm. Compound microscopes have multiple lenses. Compound microscopes have two stage adjustment knobs.
Compound microscopes have multiple lenses.
Which of the following microscopes uses a laser beam? Confocal Interference Bright-field Fluorescence
Confocal
When a culture shows two or more microbes of uncertain identity that have been introduced into it, the culture is considered to be which of the following? Contaminated Mixed Axenic/pure
Contaminated
Which of the following staining procedures employ both a primary dye and a counterstain? Simple stain Differential stain Capsule stain
Differential stain
Which of the following has the greatest magnification power? Dark-field microscope Light microscope Electron microscope They are all the same! Fluorescent microscope
Electron microscope
Identify a medium which contains complex organic substances and/or special growth factors which allows even fastidious organisms to thrive. Enriched medium General medium Selective medium
Enriched medium
Media ingredients that cannot be chemically defined include which of the following? Multiple select question. (3) Extracts of animals, plants, or yeasts Disodium hydrogen phosphate Blood and serum Sodium chloride Cell secretions
Extracts of animals, plants, or yeasts. Blood and serum. Cell secretions.
Light-based microscopes can obtain greater magnification than electron microscopes. True False
False
Dark structures as seen using transmission electron microscopy correspond to which of the following? Higher densities Lower densities
Higher densities
What can be used to enhance the amount of light passing through the specimen and the many lenses to reach the eye? Immersion oil Water Decrease the temperature Safranin Crystal violet
Immersion oil
What can be used to enhance the amount of light passing through the specimen and the many lenses to reach the eye? Water Immersion oil Safranin Crystal violet Decrease the temperature
Immersion oil
Which of the following describes the process of placing a culture of microorganisms in a temperature-controlled chamber to encourage growth? Incubation Inoculation Identification Inspection
Incubation
Identify a dye that results in a negative stain. Alcohol Cyanosin India ink Eosin
India ink
Which can be used to produce a negative stain? Multiple select question.(2) Iodine India ink Safranin Nigrosin Crystal violet
India ink Nigrosin
Which one of the following is not part of the six basic techniques used to grow, examine and manipulate microorganisms in the laboratory? Infection Inspection Inoculation Isolation
Infection
Which term identifies the introduction of microorganisms into an environment in which they will multiply? Inoculation Inspection Identification Seeding Incubation
Inoculation
Which of the following are required for proper isolation of microorganisms? Multiple select question. Inoculation tools Agar in a petri dish Nutrient broth in a tube A syringe
Inoculation tools Agar in a petri dish
Which of the following six "I'"s deals with the evaluation of cultures both macroscopically and microscopically? Isolation Inspection Inoculation Identification Incubation
Inspection
Identify the four types of visible-light microscopes. Multiple select question.(4) Confocal Scanning tunneling Atomic force Interference Dark-field Bright-field Phase-contrast
Interference Dark-field Bright-field Phase-contrast
Which describes any technique that results in individual bacteria separate from others? Isolation Incubation Infection Inoculation
Isolation
A scanning confocal microscope is characterized by which of the following? Its use of ultraviolet radiation to scan the specimen Its use of a laser beam to scan the specimen Its use of a beam of electrons to scan the specimen
Its use of a laser beam to scan the specimen
The main difference(s) between light microscopes and electron microscopes is(are) which of the following? Multiple select question. Light microscopes transmit light through the specimen for visualization. Light microscopes use a light beam to scan the specimen. Light microscopes are many times more powerful. Electron microscopes are many times more powerful. Electron microscopes use a beam of electrons to scan the specimen.
Light microscopes transmit light through the specimen for visualization. Electron microscopes are many times more powerful. Electron microscopes use a beam of electrons to scan the specimen.
Magnification results from the interaction of which of the following? Optical aperture and light source Lens curvature and optical aperture Light waves and lens curvature
Light waves and lens curvature
How are broths best categorized? Liquid, water based, and able to solidify at temperatures above freezing Liquid, water based, solid below 46ο C but liquid above 55ο C Liquid, water based, and not able to solidify at temperatures above freezing
Liquid, water based, and not able to solidify at temperatures above freezing
A wet mount is suitable for observing which of the following? Dry, motile or non-motile cells Stained, motile or non-motile cells Living, motile or non-motile cells
Living, motile or non-motile cells
Which of the following identifies the method that requires you to serially dilute microorganisms by passing them from one tube of cool but still liquid agar to another, and then eventually pouring them into petri plates with the intended purpose of obtaining individual colonies? Spread plate method Loop dilution method Streak plate method
Loop dilution method
Heat fixing a sample in microscopy is done for which purpose? Multiple select question. (3) Making a more permanent mount for long-term study Highlighting cell structure for observation Drying the liquid used to make the smear Preserving various cellular components in a natural state Simultaneously killing and securing the sample to the slide
Making a more permanent mount for long-term study Preserving various cellular components in a natural state Simultaneously killing and securing the sample to the slide
Why is identification of bacteria on the macroscopic appearance limited? Many bacteria will carry out the same biological reactions. Bacterial growth is rarely visible on a macroscopic level. Many bacteria exhibit similar colony growth.
Many bacteria exhibit similar colony growth.
Identify the statement which accurately describes the six "I"s for the culturing of microorganisms. Microbes must be grown in pure culture Contamination is not a concern during inspection. The microbe collection must not be contaminated.
Microbes must be grown in pure culture
Which of the following is an example of semisoild agar? Urea broth Nutrient broth Motility agar Nutrient agar
Motility agar
Which of the following is absolutely true for all viruses? Must grow in or on living cells Must be resistant to light Must be airborne
Must grow in or on living cells
Which of the following staining procedures produces a reverse image of that created by a positive stain, thus forming a silhouette around the microorganism's outer boundary? Negative stain Differential stain Simple stain Gram stain
Negative stain
Agar as a media solidifying agent has many benefits including which of the following? Multiple select question.(4) Not harmful to microbes or humans Being flexible and moldable Not digestible as a nutrient by most microbes Providing a nutrient source to the media Retaining moisture and nutrients
Not harmful to microbes or humans. Being flexible and moldable. Not digestible as a nutrient by most microbes. Retaining moisture and nutrients.
Which lens forms the initial image of the specimen viewed under a light microscope? Condenser Ocular Objective Iris
Objective
Which of the following are applications of bright-field microscopy? Observation of live and preserved stained specimens Observation of dark specimens in a white field Observation of fluorescent samples Observation of the internal structure of viral particles
Observation of live and preserved stained specimens Observation of dark specimens in a white field
Organelles, granules, and bacterial endospores are best observed using which microscope? Phase-contrast Dark-field Bright-field Scanning-electron
Phase-contrast
Which of the following microscopes provide detailed views of unstained, live specimens? Multiple select question. Phase-contrast microscope Dark-field microscope Differential interference microscope Electron microscope Bright-field microscope
Phase-contrast microscope Dark-field microscope Differential interference microscope Bright-field microscope
Which of the following represent inoculation of a microbial sample? Multiple select question. Sequencing of the microbial DNA Placing a sample in sterile medium Examining a microbe under the microscope Maintaining a sample at body temperature Streaking a sample on an agar plate
Placing a sample in sterile medium Streaking a sample on an agar plate
________ staining involves any staining technique in which the dye actually sticks to the specimen and gives it color.
Positive
Proper use in aseptic technique results in which of the following? Multiple choice question. Good size of microbial organisms on the medium Introduction of only beneficial microbes to the growth medium Good microbial growth in the medium Prevention of the introduction of unwanted microbes into the sterile medium
Prevention of the introduction of unwanted microbes into the sterile medium
Which of the following is the initial image of a specimen formed by the objective lens? Fixed image Real image Virtual image
Real image
What color do Gram-negative bacteria exhibit after Gram staining? Red Blue Green Purple
Red
Which of the following describes a media which is designed to remove or decrease the penetration of oxygen into the media? Oxidizing media Sequestering media Deoxy-media Reducing media
Reducing media
Which of the following ingredients makes Mannitol Salt Agar (MSA) selective for Staphylococcus species? Mannitol Crystal violet Agar Salt
Salt
_______ media contain compounds which limit the growth of some microorganisms.
Selective
Which of the following wavelengths should give the best resolution? Long wavelengths Ultra long wavelengths Short wavelengths
Short wavelengths
Identify disadvantages of wet mount preparations. Multiple select question. Slide very susceptible to drying Damage to large cells by cover glass Easy contamination of handler's fingers Dyes easily transferred to handler's fingers. Living cells cannot be visualized with wet mounts.
Slide very susceptible to drying Damage to large cells by cover glass Easy contamination of handler's fingers
In brain heart infusion broth (a complex, nonsynthetic media), none of the ingredients can be chemically defined, except which of the following? Brain extract Peptone extract Sodium chloride Beef extract
Sodium chloride
At room temperature, agar will always be which of the following? Selective Liquid Solid
Solid
Which of the following is not true for viruses? Must grow within a cell Cannot replication on their own Some viruses can be grown in chicken eggs Some viruses can be grown in nutrient agar
Some viruses can be grown in nutrient agar
A sterile tool called a "hockey stick" is used in which bacterial isolation technique? Spread plate Loop dilution Streak plate Pour plate
Spread plate
Identify the inoculation technique that uses a sterile tool, such as an L-shaped glass rod, to distribute the sample evenly around the surface of a plate? Streak plate technique Pour plate technique Loop dilution technique Spread plate technique
Spread plate technique
Which of the following is unique to dark-field microscopes? Coarse adjustment knob Ocular lens Objective lens Stop-condenser Nosepiece
Stop-condenser
Identify the method of spreading a small culture sample over a solid medium in a pattern that gradually thins out the sample resulting in isolated colonies. Inoculation plate method Loop dilution/pour plate method Spread plate method Streak plate method
Streak plate method
Which of the following describes a second-generation culture made from a well-established colony of microorganisms? Pure Primary Subculture Axenic
Subculture
Which of the following media are chemically definable? Nonsynthetic Synthetic
Synthetic
Identify all of the following conditions which modern incubators can regulate. Multiple select question.(3) Temperature Moisture Salinity Atmosphere conditions (Gases)
Temperature Moisture Atmosphere conditions (Gases)
Which is the difference between Gram and acid-fast stains? The Gram stain is differential while the acid-fast stain is not. The acid-fast stain is differential while the Gram stain is not. The acid-fast stain shows cell shapes while the Gram stain does not. The Gram stain uses crystal violet while the acid-fast stain uses carbol fuschin.
The Gram stain uses crystal violet while the acid-fast stain uses carbol fuschin.
The type of mount in which a specimen is prepared for optical microscopy is dependent upon which of the following? Multiple select question.(3) The condition of the specimen The type of microscopy available The aims of the examiner The number of microbes present Whether the microbe is pathogenic or not
The condition of the specimen The type of microscopy available The aims of the examiner
The streak plate technique works by using an inoculating loop to spread a bacterial sample in a pattern that thins it out but this only works if which of the following is done? Multiple choice question. The culture is pure to begin with. The inoculating loop is sterilized only at the beginning of the pattern. The inoculating loop is re-sterilized at each thinning step of the pattern. There is a new diluted sample added at each step.
The inoculating loop is re-sterilized at each thinning step of the pattern.
How does the loop dilution/pour plate technique result in colony isolation? Spread on the surface The sample is partially killed by the melted agar. The sample is serially diluted before dispensing into petri dishes. Introduced by a needle allowing isolated subsurface growth
The sample is serially diluted before dispensing into petri dishes.
General-purpose media are characterized by which of the following? They are synthetic and chemically definable. They allow the growth of a broad range of microbes. They contain only one nonsynthetic nutrient.
They allow the growth of a broad range of microbes.
Why can dyes be used as differential agents in media? They are pH indicators. They have inhibitory properties. They are weak salts. They serve as growth factors.
They are pH indicators.
Which describes fastidious organisms? They require simple nutrients when grown in culture. They grow on minimal media. They require growth factors and/or complex nutrients.
They require growth factors and/or complex nutrients.
Which of the following describes general-purpose media? Multiple select question.(4) They usually contain a mixture of nutrients. They are usually chemically defined synthetic media. They are usually nonsynthetic. They include nutrient agar and broth. They grow a broad spectrum of microbes. They include Mannitol salt and MacConkey agar.
They usually contain a mixture of nutrients. They are usually nonsynthetic. They include nutrient agar and broth. They grow a broad spectrum of microbes.
What is the method of choice to view detailed structural characteristics of both cells and viruses? Fluorescence microscopy Transmission electron microscopy Phase-contrast microscopy
Transmission electron microscopy
True or false: The specimen used in SEM is metal-coated before it is bombarded with electrons.
True
A fluorescent microscope uses which of the following to view a specimen? Ultraviolet light Visible light An atomic probe An electron beam
Ultraviolet light
What term is used to describe the image generated by the ocular lenses of a microscope? Virtual image Actual image Real image
Virtual image
Which of the following are obligate parasites? Multiple select question. Most bacteria Yeasts Viruses Rickettsias Fungi
Viruses Rickettsias
The resolving power of a microscope is calculated by dividing the wavelength of light by double the _______ of the objective lens. length refraction magnification power numerical aperture
numerical aperture
The resolving power of a microscope is calculated by dividing the wavelength of light by double the _______ of the objective lens. length refraction numerical aperture magnification power
numerical aperture
A collection of bacteria on a loop which is thinly spread across a glass slide, allowed to air-dry, and heat fixed is called a ________.
smear
The procedure that involves applying colored cationic or anionic chemicals (dyes) to specimens is called ________.
staining
Magnification of an object or specimen by a compound microscope occurs in how many phases? 3 4 5 1 2
2
The ________ image of a microscopic specimen is the one that will be received by the eye and converted to a retinal and visual image.
virtual
