GCD Chapter 24

A gene knockout is a gene a. whose function has been inactivated b. that has been transferred to a different species c. that has been moved to a new location in the genome d. that has been eliminated from a species during evolution

whose function has been inactivated

Which of the following can be analyzed using a protein microarray? a. The amounts of particular proteins made by a sample of cells b. Protein function c. Protein-protein interactions d. All of the above

All of the above

Which of the following is a reason why the proteome of a eukaryotic cell is usually much larger than its genome? a. Alternative splicing b. RNA editing c. Posttranslational covalent modifications d. All of the above are reasons for the larger size of a proteome

All of the above are reasons for the larger size of a proteome

For the method of RNA sequencing (RNA-Seq), which of the following is the correct order of steps? a. Isolate RNAs, synthesize cDNAs, fragment RNAs, sequence cDNAs, align cDNA sequences b. Synthesize cDNAs, sequence cDNAs, isolate RNAs, fragment RNAs, align cDNA sequences c. Isolate RNAs, fragment RNAs, synthesize cDNAs, sequence cDNAs, align cDNA sequences d. Synthesize cDNAs, isolate RNAs, fragment RNAs, sequence cDNAs, align cDNA sequences

Isolate RNAs, fragments RNAs, synthesize cDNAs, sequence cDNAs, align cDNA sequences

During two-dimensional gel electrophoresis, proteins are separated based on a. their net charge at a give pH b. their mass c. their ability to bind to a specific resin d. both a and b

both a and b

Homologous genes a. are derived from the same ancestral gene b. are likely to carry out the same or similar functions c. have similar DNA sequences d. exhibit all of the above features

exhibit all of the above features

The BLAST program begins with a particular sequence and a. translates it into an amino acid sequence b. determines if it contains one or more genes c. identifies homologs within a database d. does all of the above

identifies homologs within a database

A DNA microarray is a slide that is dotted with a. mRNAs from a sample of cells b. fluorescently labeled cDNA c. known sequences of DNA d. known cellular proteins

known sequences of DNA

The identification of a stop codon for a particular gene is an example of a. sequence recognition b. pattern recognition c. both a and b d. none of the above

sequence recognition

The technique of tandem mass spectroscopy is used to determine a. the amino acid sequence of a peptide fragment b. the nucleotide sequence of a segment of RNA c. the nucleotide sequence of a segment of DNA d. the number of genes in a species' genome

the amino acid sequence of a peptide fragment

The purpose of a ChIP-chip assay is to determine a. the expression levels of particular genes in the genome b. the sites in a genome where a particular protein binds c. the amount of a specific protein that is made in a given cell type d. any of the above

the sites in a genome where a particular protein binds