Microbiology Lab Chapter 5

Which metric units are most appropriate for expressing the size of macroscopic organisms? Nanometers Centimeters Micrometers Meters

Centimeters Meters

What is enhanced when a stain is used to make a sample, or parts of a sample, darker than the background in a slide? Media type Resolution Magnification Contrast

Contrast

Which metric unit is most appropriate for expressing the size of human skin cells? Micrometer Centimeter Meter Nanometer

Micrometer

Which microscopy method is beneficial because of its applications for living specimens? Wet mount Scanning electron Oil immersion Magnetic resonance imaging

Wet mount

Resolution is an image quality factor that involves being able to see fine _____, which is essential for image clarity.

details or detail

Changing the magnification of an image with a microscope changes the portion of the slide that is visible, or the stage. field of view. real image. objective.

field of view.

Bright-field microscopes use a combination of visible and invisible light to magnify an image tremendously. light and a screen to project a larger image of a specimen. electrons and a high-powered scanner to produce an image of the specimen. glass lenses and light to view a specimen.

glass lenses and light to view a specimen.

Bright-field microscopes use a combination of lenses and _____ to view a specimen.

lights, light, light sources, a light source, or a light

The portion of a slide that is visible through the eye piece is called the field of _____.

view or views

A microscopy sample that is prepared by suspending it in a fluid that temporarily keeps it alive and gives it space to move is called a _____ _____.

wet mount

A benefit of using the oil immersion lens is a reduction in scatter, which increases resolution. decreases resolution. decreases contrast. increases contrast.

increases resolution.

If a specimen is viewed using a 100× objective and the ocular lenses, what is the total magnification? 101× 1100× 1000× 110×

1000×

Oil immersion microscopy uses which objective lens? 10× lens 100× lens 40× lens 4× lens

100× lens

Which lens has a magnification of 40×? Scanning objective Oil immersion High-power objective Low-power objective

High-power objective

The lenses of a bright-field microscope are responsible for the _____ of the object you are viewing. Multiple choice question. Magnification Media type Contrast Resolution

Magnification

Which image quality factor is defined as the ability to make an object appear larger? Contrast Media type Magnification Resolution

Magnification

Which of the image quality factors is usually expressed in a number such as 40×? Magnification Resolution Media type Contrast

Magnification

Select the benefits of wet mount sample preparation for microscopy. Motility of specimens can be observed. This preparation reduces light scatter. Living organisms can be viewed. The preparation is relatively simple and fast.

Motility of specimens can be observed. Living organisms can be viewed. The preparation is relatively simple and fast.

Which image quality factor is defined as the ability to distinguish fine detail? Magnification Media type Resolution Contrast

Resolution

Which lens has a magnification of 4×? Oil immersion High-power objective Low-power objective Scanning objective

Scanning objective

Which part of the bright-field microscope is a lens that is located closest to the eye during use? The objective lenses The ocular lenses The aperture The condenser lens

The ocular lenses

The goal of microscopy is to create a magnified image of objects that are too small to be seen with the unaided eye. any object. objects that are generally measured in centimeters or meters. objects that are too large to be seen in detail.

objects that are too small to be seen with the unaided eye.