the microscope
Ocular lenses
"EYEPIECES" ARE LOCATED ON TOP OF THE BODY TUBE AND USUALLY HAVE A MAGNIFICATION POWER OF 10X.
IF THE SIZE OF THE HIGH-POWER FIELD IS 1.2 MM, AND OBJECT THAT OCCUPIES APPROXIMATELY A THIRD OF THAT FIELD HAS AN ESTIMATED DIAMETER OF
.4MM
IF, WHEN USING A 10X OCULAR AND A 15X OBJECTIVE, THE FIELD SIZE IS 1.5 MM, THE APPROXIMATE FIELD SIZE WITH A 30X OBJECTIVE IS
.75MM
IF A MICROSCOPE HAS A 10X OCULAR AND THE TOTAL MAGNIFICATION AT THE PARTICULAR TIME IS 950X, THE OBJECTIVE LENS IS USE AT THE TIME IS
95X
DO THE FOLLOWING FACTORS INCREASE OR DECREASE AS ONE MOVES TO HIGHER MAGNIFICATIONS WITH THE MICROSCOPE? DEPTH OF FIELD ____
DECREASE
DO THE FOLLOWING FACTORS INCREASE OR DECREASE AS ONE MOVES TO HIGHER MAGNIFICATIONS WITH THE MICROSCOPE? WORKING DISTANCE ___
DECREASE
condenser
DELIVERS A CONCENTRATED BEAM OF LIGHT TO THE SPECIMEN
WHEN FOCUSING, ALWAYS FOCUS (TOWARD) THE SPECIMEN.
F:FALSE; AWAY (SO YOU WONT GET TO CLOSE AND BREAK THE SLIDE)
A COVERSLIP SHOULD ALWAYS BE USED (WITH WET MOUNTS AND THE HIGH-POWER AND OIL LENSES).
F:FALSE; WITH WET MOUNTS only
THE MICROSCOPE SHOULD BE STORED WITH THE (OIL IMMERSION) LENS IN POSITION OVER THE STAGE.
FALSE; LOWEST-POWER OBJECTIVE
THE MICROSCOPE LENS MAY BE CLEANED (WITH ANY SOFT TISSUE).
FALSE; ONLY WITH SPECIAL GRIT-FREE LENS PAPER
DO THE FOLLOWING FACTORS INCREASE OR DECREASE AS ONE MOVES TO HIGHER MAGNIFICATIONS WITH THE MICROSCOPE? AMOUNT OF LIGHT NEEDED ___
INCREASE
DO THE FOLLOWING FACTORS INCREASE OR DECREASE AS ONE MOVES TO HIGHER MAGNIFICATIONS WITH THE MICROSCOPE? RESOLUTION ___
INCREASE
IF, AFTER FOCUSING IN LOW POWER, ONLY THE FINE ADJUSTMENT NEED BE USED TO FOCUS THE SPECIMEN AT THE HIGHER POWERS, THE MICROSCOPE IS SAID TO BE
PARFOCAL
Stage
PLATFORM ON WHICH THE SLIDE RESTS FOR VIEWING
mechanical stage or spring clips
SECURE(S) THE SLIDE TO THE STAGE
fine-adjustment knob
SED FOR PRECISE FOCUSING ONCE INITIAL FOCUSING HAS BEEN DONE
DESCRIBE THE PROPER PROCEDURE FOR PREPARING A WET MOUNT.
THE OBJECT IS PLACED IN A DROP OF WATER (OR SALINE) ON A CLEAN SLIDE. 2. A COVER-SLIP IS HELD AT A 45 DEGREE ANGLE WITH THE FINGERTIPS. 3. IT IS LOWERED CAREFULLY OVER THE WATER AND THE OBJECT.
WHY SHOULD THE LIGHT BE DIMMED WHEN LOOKING AT LIVING (NEARLY TRANSPARENT) CELLS?
TO PROVIDE MORE CONTRAST
iris diaphragm
USED TO INCREASE THE AMOUNT OF LIGHT PASSING THROUGH THE SPECIMEN
EXPLAIN THE PROPER TECHNIQUE FOR TRANSPORTING THE MICROSCOPE.
WHEN TRANSPORTING THE MICROSCOPE, HOLD IT IN AN UPRIGHT POSITION WITH ONE HAND ON ITS ARM AND THE OTHER SUPPORTING ITS BASE. AVOID SWINGING THE INSTRUMENT DURING ITS TRANSPORT AND JARRING THE INSTRUMENT WHEN SETTING IT DOWN.
Objective Lens
adjustable lens system that permits the use of a scanning lens. low-power lens, higher power lens, oil immersion lens
movable nosepiece
carries the objective lenses; rotates so that the different objective lenses can be brought into position over the specimen
THAT AREA OF THE SPECIMEN SEEN WHEN LOOKING THROUGH THE MICROSCOPE IS THE
fiel
Body Tube
holds the ocular lens
coarse-adjustment knob
knob used to bring the object into approximate focus; used only with scanning power objective
ASSUME THERE IS AN OBJECT ON THE LEFT SIDE OF THE FIELD THAT YOU WANT TO BRING TO THE CENTER (THAT IS, TOWARD THE APPARENT RIGHT). IN WHAT DIRECTION WOULD YOU MOVE YOUR SLIDE?
left
Nosepiece
revolving device that holds objective lens
Scanning objective
short objective lens and is used to scan the whole slide
Arm
supports upper parts and provides a carrying handle
base
the flat surface of the microscope that rests on the table
Low-power objective
this is lens is longer than the scanning power objective lens and is used to view objects in greater detail
high-pwer objective
this lens will be the longest and is used to view an object in even greater detail
WHEN BEGINNING TO FOCUS, USE THE (LOWEST-POWER) LENS.
true
THE DISTANCE FROM THE BOTTOM OF THE OBJECTIVE LENS IN USE TO THE SPECIMEN IS CALLED THE
working distance
