Bio 203 Homework #10

In order to insert a human gene into a plasmid, both must _____ have identical DNA sequences originate from the same type of cell code for the same gene product be cut by the same restriction enzyme be the same length

be cut by the same restriction enzyme

How many DNA molecules would there be after four rounds of PCR if the initial reaction mixture contained two molecules? 32 16 8 64

32

What is the polymerase chain reaction (PCR)? A method to propagate a gene in bacteria. A method to amplify a fragment of DNA. A method to join two fragments of DNA together. A method to determine the sequence of bases in a gene.

A method to amplify a fragment of DNA.

During which step in the PCR cycle do primers form bonds with a single-stranded template? Denaturation. Extension. All of these steps. Annealing.

Annealing.

What enzyme forms covalent bonds between restriction fragments? DNA primase DNA helicase single-strand binding protein DNA polymerase DNA ligase

DNA ligase

During which step in the PCR cycle are nucleotides used? Annealing. Extension. Denaturation. All of these steps.

Extension.

True or false? The Taq enzyme is a type of DNA polymerase that allows researchers to separate the DNA strands during the annealing step of the PCR cycle without destroying the polymerase.

False

True or false? Comparison of the sequences of the same gene across species can give some insight into the existence of a common ancestor with that gene.

True

What information can not be obtained from the sequence of a gene? Whether the gene is methylated. Amino acid sequence of the protein. Effects of mutation on gene function. Relationship between two species.

Whether the gene is methylated.

In gel electrophoresis DNA molecules migrate from _____ to _____ ends of the gel. acidic ... basic negative ... positive basic ... acidic long ... short positive ... negative

negative ... positive

The unpaired nucleotides produced by the action of restriction enzymes are referred to as _____. sticky ends base sequences single strands restriction fragments ligases

sticky ends