Chapter 24 Genetics
DNA microarrays contain A. single-stranded DNA. B. double-stranded DNA. C. genomic DNA. D. complementary DNA.
A. single-stranded DNA.
One reason the proteome is larger than the genome is A. mutations of genes results in different proteins. B. some mRNAs are alternatively spliced. C. some mRNAs are not expressed due to regulation by miRNAs. D. some genes are silenced through methylation.
B. some mRNAs are alternatively spliced.
RNA-seq involves directly sequencing the RNA. True False
False
DNA microarrays are used to study patterns of gene expression. True False
True
DNA microarrays may be used to study how a cell responds to specific environmental conditions. True False
True
Computer programs that analyze genetic sequences can look for sequences or patterns. Which statement is correct about these types of searches? A. Typically, both sequence and pattern recognition searches require the researcher to input a sequence of interest. B. Typically, neither sequence nor pattern recognition searches require the researcher to input a sequence of interest. C. Typically, sequence recognition searches require a researcher to input a sequence of interest but a pattern recognition search does not. D. Typically, pattern recognition searches require a researcher to input a sequence of interest but a sequence recognition
A. Typically, both sequence and pattern recognition searches require the researcher to input a sequence of interest.
In the microarray procedure, what molecule is labeled with a fluorescent tag? A. mRNA B. double-stranded DNA C. single-stranded cDNA D. double-stranded cDNA
C. single-stranded cDNA
To use a microarray, mRNA from cells of interest are first converted to A. double-stranded DNA. B. proteins. C. tRNA. D. cDNA.
D. cDNA.
Choose true or false for each statement. 1. Two dimensional gel electrophoresis separates proteins based on size alone. (Click to select) True False 2. During isoelectric focusing proteins are separated by the pH at which they have a net neutral charge. (Click to select) True False 3. SDS coats the proteins to give them a net positive charge. (Click to select) True False 4. SDS allows proteins to separate based on molecular mass. (Click to select) True False 5. Two dimensional electrophoresis allows proteins that differ by one charged amino acid to be separated. (Click to select) True False
False 1. True 2. False 3. True 4. True
Chromatin immunoprecipitation involves A. Chemical cross linking of DNA to protein. B. Antibodies to specific DNA sequences for immunoprecipitation. C. Crosslinking of proteins to other proteins. D. Protein sequencing.
A. Chemical cross linking of DNA to protein.