Labster - Bacterial Isolation

Which bacterial species is contained in the blood sample from Max? a) Proteus b) e. coli c) Fungi d) Salmonella

d) Salmonella

What conclusion can you draw from the graph?

The wire loop is not sterile

Based on the results of the streaking method and previously inoculated plate, how can you tell that the colony was successfully isolated? a) The bacterial colony is deposited a few millimeters away from other colonies on the surface of the agar plate with uniform, clearly defined shape and color b) The bacterial colony is mixed with other colonies on the surface of the agar plate with nonuniform shape and color c) The bacterial colony is within the cluster of other colonies on the surface of the agar plate with similar shapes and colors d) It cannot be determined just by looking at the plate

a) The bacterial colony is deposited a few millimeters away from other colonies on the surface of the agar plate with uniform, clearly defined shape and color

Why is it important for us to isolate a single colony of bacteria from the chicken farm sample? a) We require a sample in which all the bacterial cells are genetically identical b) A single colony will show the bacteria are resistant to antibiotics c) If a single colony grows we know it's the same strain of bacteria that infected Max d) We need to identify if the bacteria are Salmonella

a) We require a sample in which all the bacterial cells are genetically identical

The flame kills all the microbes and sterilizes the wire loop. Why is this important to do before working with the chicken farm sample? a) To reduce the spread of antibiotic resistance b) To avoid contamination c) Salmonella can be found everywhere d) All the answers are correct

b) To avoid contamination

Which method can we use to identify if the antibiotic-resistant bacteria in the chicken shed sample are Salmonella? a) Prescribe Max with a course of antibiotics that treat Salmonella b) Culture the bacteria in antibiotics c) Grow the bacteria on a Salmonella Shigella agar plate d) Isolate a colony of bacteria on a blood agar plate

c) Grow the bacteria on a Salmonella Shigella agar plate

Why are the bacteria samples stored in the fridge? a) They are pathogenic b) Otherwise they get contaminated c) Reduces bacterial growth d) Bacteria cannot survive at room temperature

c) Reduces bacterial growth

There are black and red antibiotic-resistant bacterial species growing on the agar plate. Can you identify these species? a) E. Coli and Shigella b) Shigella and Salmonella c) Salmonella and E. Coli d) Shigella and Proteus

c) Salmonella and E. Coli

Which statement best describes the purpose of the T-streaking technique? a) To reduce the risk of contamination on the agar surface b) To boost bacterial growth on the agar surface c) To decrease the concentration of bacterial cells on the agar surface d) To grow 3 different strains of bacteria on each sector

c) To decrease the concentration of bacterial cells on the agar surface