Micro test 4 and 5
What temperature is an optimal growth temperature for Campylobacter jejuni?
42C
What is the name of the organism that causes the disease cholera?
V. cholerae
A patient is brought to the emergency department witha severe case of gastroenteritis. The patient experienced gastrointestinal upset 3 days after eating raw oysters. What organism can be the culprit for this patients condition?
V. parahaemolyticus
What is the name of the organism that is responsible for "SUMMER DIARRHEA" in japan?
V. parahaemolyticus
WHich of the following is a CURVED gram negative rod that grows on TCBS agar?
Vibrio
A stool sample from a patient who recently traveled to India gave the following colonies when the stool was plated on TCBS. What organism is most likely growing in the image below?
Vicrio cholerae
Which of the following agars is not selective for Enterobacteriaceae
Buffered charcoal yeast extract
Which nonfermenter is considered by government agencies to be a potential bioterrorist agent?
Burkholderia mallei
What organism has a microscopic morphology of tiny, CURVED, Gram negative rods, wtih S SHAPES or seagull wing shapes on Gram stain?
Campylobacter
What organism may play a role in Guillain-Barre syndrome ?
Campylobacter
What organism is the most common cause of bacterial gastroenteritis worldwide
Campylobacter jejuni
Escherichia coli strains have all of the following properties EXCEPT
Can use citrate as a sole carbon source
When collecting specimens that may contain Campylobacter spp. bacteria. What transport medium should they be placed in if a delay in transport is possible
Cary-Blair
What organism is an opportunistic pathogen that strikes the immunocompromised patient with neutrophil deficits and produces violet pigment on nonselective agar
Chromobacterium violaceum
A microbiologist is reading stool culture plates. She is looking for enteric pathogens on the MAC plate. What do they look like?
Clear colorless colonies
Campy-BAP contains
Brucella agar base, 10% sheep red blood cells, vancomycin, trimethoprim, polymyxin B, amphotericin B, and cephalothin
Swarming is
A hazy blanket of growth on the surface of the agar that extends way beyond the streak lines
An organism gave the following reactions: TSI: acid slant/acid butt; no H2S gas produced indole: positive motility: positive citrate: negative lysine decarboxylase: positive Urea: negative VP: negative This organism most likely is: a Klebsiella pneumoniae b Shigella dysenteriae c Escherichia coli d Enterobacteria cloacae
Escherichia coli
A microbiologist is reading stool culture plates. She sees an organism that has a dry, pink colony with surrounding "halo" or pink on the MAC. What is a good presumptive identification of this organism?
Escherichia/Citrobacter- like organism
What organism is the most common cause of UTI in humans
Eschericia coli
In the carrier state, where are pathogenic salmonella carried
Gallbladder
While swimming in a lake near his home, a young boy cut his foot, and an infection developed. The culture grew a nonfastidious gram-negative, oxidase positive, beta-hemolytic, motile bacilli that produced deoxyribonuclease. The most likely identification is: a Enterobacter cloacae b Serratia marcescens c Aeromonas hydrophila d Escherichia coli
Aeromonas hydrophilia
A microbiologist is reading the plates from a sputum culture. On the SBA, they see FLAT spreading colonies with a metallic sheen. On cetrimide agar, a fluorescent green color is seen in the media with clear colonies. On MAC, medium clear colonies are seen that have a FRUITY or grape like odor. What is the most likely organism?
P. aeruginosa
What organism causes pulmonary disease among individuals with cystic fibrosis?
P. aeruginosa
What two bacteria produce swarming colonies on nonselective media, such as SBA?
P. vulgaris and P. mirabilis
The primary pathogens of the Enterobacteriaceae include
Proteus mirabilis
What organism possesses the Vi antigen
Salmonella typhi
A TSI tube inoculated with an organism gave the following reactions: Alkaline slant/acid butt: no H2S, no gas produced This organism is?
Shigella dysenteriae
A positive test for motility
Shows hazy appearance throughout or movement away from a stab
Why is that most cases of salmonella food poisioning are not treated with antibiotics
The disease is self limiting and will subside on its own
Why can direct microscopy not be used to provide a presumptive identification of the enteric bacteria?
The microscopic characteristics are indistinguishable from other gram negative bacteria
What substances do non fermeters fail to ferment in traditional media
carbohydrates
How is chlorea treated
copious amounts of intravenous fluid
All of the following are symptoms of salmonellosis that may appear 8 to 36 hours after ingestion of contaminated food EXCEPT
headache
If a technician sees a lactose fermenter on the MAC plate, what is the first biochemical test performed
indole
A nonfermenting gram negative bacilius is isolated from a wound. The nitrate and oxidase are strongly positive. The growth on sheep blood agar has a GRAPE LIKE odor. The organism is
pseudomonas aeruginosa
What nonfermenter is the leading cause of nosocomial pneumonia and bacteremia?
pseudomonas aeruginosa
A blood culture bottle with macroscopic signs of growth is gram stained and the technician notes small, curved gram negative bacilli resembling "gull wings". It is subcultured to blood and chocolate agar, and incubated aerobically and anaerobically. After 24 hours, no growth is apparent. The next step should be to
subculture the bottle; and incubate in microaerophilic conditions
A gastroenterologist submits a gastric biopsy from a patient with a peptic ulcer. To obtain presumptive evidence of Helicobacter pylori, a portion of the specimen should be added to which media
urea broth
How is Helicobacter pylori identified?
A rapid urease test done on a gastric biopsy
What organism causes meliodosis
B. pseudomallei
What is the name of the organism that is strongly associated with gastric, peptic, and duodenal ulcers as well as GI carcinoma
Helicobacter pylori
A microbiologist is reading stool culture plates. she sees an organism that has a large, mucoid pink colony on MAC. What is a good presumptive identification of this organism?
Klebsiella/ Enterobacter like organiam
The Eschericia coli serotype 0157:H7 is associated with which of the following?
Liver damage
What kind of atmosphere do Camplobacter require for growth?
Microaerophilic and capnophilic
The initial screening of gram negative rods is done by testing for the use of the carbohydrate
lactose
All of the following are biochemical features of Samonella EXCEPT
lactose positive
What type of an atmosphere do camplobacters require for growth
microaerophilic and capnophilic
What biochemical test will differentiate nonfermenters from Enterobacteriaceae ?
oxidase
What is the first test that should be performed on a NON lactose fermenter
oxidase
