Micro test 4 and 5

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What temperature is an optimal growth temperature for Campylobacter jejuni?

42C

What is the name of the organism that causes the disease cholera?

V. cholerae

A patient is brought to the emergency department witha severe case of gastroenteritis. The patient experienced gastrointestinal upset 3 days after eating raw oysters. What organism can be the culprit for this patients condition?

V. parahaemolyticus

What is the name of the organism that is responsible for "SUMMER DIARRHEA" in japan?

V. parahaemolyticus

WHich of the following is a CURVED gram negative rod that grows on TCBS agar?

Vibrio

A stool sample from a patient who recently traveled to India gave the following colonies when the stool was plated on TCBS. What organism is most likely growing in the image below?

Vicrio cholerae

Which of the following agars is not selective for Enterobacteriaceae

Buffered charcoal yeast extract

Which nonfermenter is considered by government agencies to be a potential bioterrorist agent?

Burkholderia mallei

What organism has a microscopic morphology of tiny, CURVED, Gram negative rods, wtih S SHAPES or seagull wing shapes on Gram stain?

Campylobacter

What organism may play a role in Guillain-Barre syndrome ?

Campylobacter

What organism is the most common cause of bacterial gastroenteritis worldwide

Campylobacter jejuni

Escherichia coli strains have all of the following properties EXCEPT

Can use citrate as a sole carbon source

When collecting specimens that may contain Campylobacter spp. bacteria. What transport medium should they be placed in if a delay in transport is possible

Cary-Blair

What organism is an opportunistic pathogen that strikes the immunocompromised patient with neutrophil deficits and produces violet pigment on nonselective agar

Chromobacterium violaceum

A microbiologist is reading stool culture plates. She is looking for enteric pathogens on the MAC plate. What do they look like?

Clear colorless colonies

Campy-BAP contains

Brucella agar base, 10% sheep red blood cells, vancomycin, trimethoprim, polymyxin B, amphotericin B, and cephalothin

Swarming is

A hazy blanket of growth on the surface of the agar that extends way beyond the streak lines

An organism gave the following reactions: TSI: acid slant/acid butt; no H2S gas produced indole: positive motility: positive citrate: negative lysine decarboxylase: positive Urea: negative VP: negative This organism most likely is: a Klebsiella pneumoniae b Shigella dysenteriae c Escherichia coli d Enterobacteria cloacae

Escherichia coli

A microbiologist is reading stool culture plates. She sees an organism that has a dry, pink colony with surrounding "halo" or pink on the MAC. What is a good presumptive identification of this organism?

Escherichia/Citrobacter- like organism

What organism is the most common cause of UTI in humans

Eschericia coli

In the carrier state, where are pathogenic salmonella carried

Gallbladder

While swimming in a lake near his home, a young boy cut his foot, and an infection developed. The culture grew a nonfastidious gram-negative, oxidase positive, beta-hemolytic, motile bacilli that produced deoxyribonuclease. The most likely identification is: a Enterobacter cloacae b Serratia marcescens c Aeromonas hydrophila d Escherichia coli

Aeromonas hydrophilia

A microbiologist is reading the plates from a sputum culture. On the SBA, they see FLAT spreading colonies with a metallic sheen. On cetrimide agar, a fluorescent green color is seen in the media with clear colonies. On MAC, medium clear colonies are seen that have a FRUITY or grape like odor. What is the most likely organism?

P. aeruginosa

What organism causes pulmonary disease among individuals with cystic fibrosis?

P. aeruginosa

What two bacteria produce swarming colonies on nonselective media, such as SBA?

P. vulgaris and P. mirabilis

The primary pathogens of the Enterobacteriaceae include

Proteus mirabilis

What organism possesses the Vi antigen

Salmonella typhi

A TSI tube inoculated with an organism gave the following reactions: Alkaline slant/acid butt: no H2S, no gas produced This organism is?

Shigella dysenteriae

A positive test for motility

Shows hazy appearance throughout or movement away from a stab

Why is that most cases of salmonella food poisioning are not treated with antibiotics

The disease is self limiting and will subside on its own

Why can direct microscopy not be used to provide a presumptive identification of the enteric bacteria?

The microscopic characteristics are indistinguishable from other gram negative bacteria

What substances do non fermeters fail to ferment in traditional media

carbohydrates

How is chlorea treated

copious amounts of intravenous fluid

All of the following are symptoms of salmonellosis that may appear 8 to 36 hours after ingestion of contaminated food EXCEPT

headache

If a technician sees a lactose fermenter on the MAC plate, what is the first biochemical test performed

indole

A nonfermenting gram negative bacilius is isolated from a wound. The nitrate and oxidase are strongly positive. The growth on sheep blood agar has a GRAPE LIKE odor. The organism is

pseudomonas aeruginosa

What nonfermenter is the leading cause of nosocomial pneumonia and bacteremia?

pseudomonas aeruginosa

A blood culture bottle with macroscopic signs of growth is gram stained and the technician notes small, curved gram negative bacilli resembling "gull wings". It is subcultured to blood and chocolate agar, and incubated aerobically and anaerobically. After 24 hours, no growth is apparent. The next step should be to

subculture the bottle; and incubate in microaerophilic conditions

A gastroenterologist submits a gastric biopsy from a patient with a peptic ulcer. To obtain presumptive evidence of Helicobacter pylori, a portion of the specimen should be added to which media

urea broth

How is Helicobacter pylori identified?

A rapid urease test done on a gastric biopsy

What organism causes meliodosis

B. pseudomallei

What is the name of the organism that is strongly associated with gastric, peptic, and duodenal ulcers as well as GI carcinoma

Helicobacter pylori

A microbiologist is reading stool culture plates. she sees an organism that has a large, mucoid pink colony on MAC. What is a good presumptive identification of this organism?

Klebsiella/ Enterobacter like organiam

The Eschericia coli serotype 0157:H7 is associated with which of the following?

Liver damage

What kind of atmosphere do Camplobacter require for growth?

Microaerophilic and capnophilic

The initial screening of gram negative rods is done by testing for the use of the carbohydrate

lactose

All of the following are biochemical features of Samonella EXCEPT

lactose positive

What type of an atmosphere do camplobacters require for growth

microaerophilic and capnophilic

What biochemical test will differentiate nonfermenters from Enterobacteriaceae ?

oxidase

What is the first test that should be performed on a NON lactose fermenter

oxidase


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