Microtomy, hone & strop, floatation
Edge first, heel to toe
Direction for honing
Edge last, toe to heel
Direction of stropping
Fine carborumdum
Hone used only for badly nicked knives
Paldwell Trefall
Invented the rocking microtome
Minot
Invented the rotary microtome
Adams
Invented the sliding microtome
Queckett
Inventex the freezing microtome
120mm
Length of biconcave knife
8"×3"
Length of hone
25mm
Length of plane concave knife
100mm
Length of plane wedge knife
1 sec
Length of time for each stroke during stropping
Horse leather
Material used in paddle strop
10-20
No. of single strokes for honing
27-32°
Normal bevel angle
10-15°
Normal clearance angle
40-120
Number of double strokes for stropping
Mineral oil
Oil that should never come in contact w strop, only w hone
Rocking microtome
Other name of cambridge microtome
Rotary microtome
Other name of minot microtome
Edges are not parallel
Reason for curved ribbon
Static electricity
Reason why sections adhere to knife
Insufficient dehydration
Reason why tissue shrinks away from was when trimmed
Repeat the whole procedure
Remedy for incomplete fixation and insufficient dehydration
76 × 25 × 1-1.2 mm
Size of glass slide
Free floating sections
These sections are fished individually using cuplets w holes
1-2mm
Thickness of paraffin around tissue block after trimming
Finely powdered aluminum oxide
This is placed on a flat circular glass plate with water to act as an abrasive for grinding and removing nicks
Platinum or copper grid (not aluminum!)
Used to fish resin
Polyethylene glycol (ex: Pearse fluid and Blank & McCarthy fluid)
Used to float out carbowax sections
Soapy water or fine oil
Used to lubricate hone before using
Vegetable oil
Used to lubricate the back of the strop before using it
Nailbrush
Used to remove black film after honing
Forceps and squirrel hair brush
Used to remove folds and creased on sections during floating out9
After honing and before cutting each block
When to strop
