BS 161 hw 5

An RNA molecule is synthesized in which direction?

5' end to 3' end

Which one of the following statements about RNA is CORRECT?

RNA uses the same purine bases as DNA.

During DNA purification from cells, a strong base, like sodium hydroxide (NaOH) is added to increase the pH dramatically. Why? (Hint: think carefully about the effect of pH on chemical group interactions (Select all that apply).

Raising the pH disrupts ionic interactions between histones and DNA. Raising the pH causes proteins in the cell, such as histones, to denature. When these proteins unfold, they lose the ability to bind DNA and thus are easily removed.

Ribose differs from deoxyribose in that a ribose:

has an extra hydroxyl group.

During transcription, the template DNA strand and the RNA:

have an anti-parallel configuration. are bound together by hydrogen bonds.

In a DNA double helix the complementary strands are held together by: Question options:

hydrogen bonds.

Transcription starts at the ________ and ends at the _________

promoter; terminator.

In prokaryotes, the messenger RNA consists of the:

unprocessed primary RNA transcript.

When RNA is transcribed from the DNA template strand, new nucleotides are added at the:

3' end.

A template DNA strand contains 30% A, 20%T, 27% G, and 23% C. The RNA transcript contains:

30% U, 20% A, 27% C, and 23% G.

Which of the following CORRECTLY describes the complementary base pairing of adenine in both DNA and RNA? Question options:

Adenine pairs with thymine in DNA and with uracil in RNA.

A promoter region is a specific:

DNA sequence where RNA polymerase can bind to initiate transcription.

According to the central dogma of molecular biology, the genetic information flows as follows:

DNA →RNA →Protein

During transcription of a given protein-coding gene, both strands are used as template. Question options:

False

Which one of the following statements about RNA is INCORRECT?

RNA is usually found in double-stranded form, just like DNA.

What is the name of the enzyme complex that forms at the start of transcription?

RNA polymerase

Transcription refers to the process in cells where information from:

a DNA strand is used to synthesize RNA.

The base uracil pairs with _______

adenine.

RNA processing occurs in:

both prokaryotes and viruses.

The RNA transcript runs from _____ and matches ______. (Select all that a

the sense (i.e., non-template) DNA strand 3' to 5' 5' to 3'

During transcription, the sequence of the primary transcript (i.e., the new produced RNA) matches (Select all that apply):

the sense strand, but T is replaced with U. the non-template strand, but T is replace with U.

Some RNA molecules possess catalytic activity.

true