Defination

Elution

- is a process wherein the separated substances on the chromatogram are actually washed off the adsorbent for the purpose of removal of the solute from the stationary phase

Solutes

Components of mlecule of the substance on the chromatogram

Sepharos

Gel is made up of agarose

Bio gel p

Is polycrylamide in nature

Water silica

Moset common size exlusion packing for use under pressure

Support

a solid base which supports the liquid film in partition chromatography

Sephadex

beads of cross linked dextran used as a stationary phase of a chromatographic system

Column

is made of glass somewhat similar to a biurette

Stationary phase

solid base powder packed into the column through which the mobile phase flows (solid)

Development

the process where mobile phase is allowed to flow over the adsorbent or support - after the substances on the chromatogram have separated by development they are being DETECTED/VISUALIZED.

Adsorbent

the solid material that serves as the stationary phase in adsorption chromatography

Eluate

the solute coming out of the column dissolved in the mobile phase

Chromagtogram resolution

to become separated into constituent parts

Sphadex

• are granular, hydrophilic, water insoluble porous material and each granule acts as a complex system of holes