GMS Stain
What is the order of the stain?
-Chromic Acid -Na bisulphite -Working Silver -AuCl -Na2S2O3 -NFR
What kind of reduction is GMS?
Argentaffin (tissue reduces itself)
What is the toner used?
AuCl
What color is glycogen, mucin, fungai?
Black
Causes oral thrush?
Candida albicans
What is in fungi cell wall?
Carbohydrates (chitin)
What is the oxidizer?
Chromic acid
Carried in pigeons?
Cryptococcus
What are the contents of the working methenamine silver?
Distilled water Methenamine - silver 5% borax
Why can't bacteria be grown in pathology lab?
Fixatives kill them
What is used for impregnation and reduction?
Methenamine silver
What is the counterstain?
NFR
What is the principle of fungal staining?
Oxidation of carbohydrates to aldehydes
What color is nuclei and cytoplasm?
Red
What is the method of fungal staining?
Silver impregnation
What is the bleach used?
Sodium bisulphite
The removes the unreduced silver?
Sodium thiosulphate
What does extended incubation cause?
Too dark tissue
What does shortened incubation cause?
Too light tissue
