GMS Stain

What is the order of the stain?

-Chromic Acid -Na bisulphite -Working Silver -AuCl -Na2S2O3 -NFR

What kind of reduction is GMS?

Argentaffin (tissue reduces itself)

What is the toner used?

AuCl

What color is glycogen, mucin, fungai?

Black

Causes oral thrush?

Candida albicans

What is in fungi cell wall?

Carbohydrates (chitin)

What is the oxidizer?

Chromic acid

Carried in pigeons?

Cryptococcus

What are the contents of the working methenamine silver?

Distilled water Methenamine - silver 5% borax

Why can't bacteria be grown in pathology lab?

Fixatives kill them

What is used for impregnation and reduction?

Methenamine silver

What is the counterstain?

NFR

What is the principle of fungal staining?

Oxidation of carbohydrates to aldehydes

What color is nuclei and cytoplasm?

Red

What is the method of fungal staining?

Silver impregnation

What is the bleach used?

Sodium bisulphite

The removes the unreduced silver?

Sodium thiosulphate

What does extended incubation cause?

Too dark tissue

What does shortened incubation cause?

Too light tissue