Micro Bio Lab Test 2

Which of the following is the correct order of steps needed to aseptically inoculate an agar plate from a broth culture? -Remove broth tube cap and flame mouth of tube -Inoculate plate -Flame loop -Obtain inoculum -Flame mouth of tube and replace cap -Flame loop

1. Flame loop 2. Remove broth tube cap and flame mouth of tube 3. Obtain inoculum 4. Flame mouth of tube and replace cap 5. Inoculate plate 6. Flame loop

Please order the steps in this figure to demonstrate your understanding of the inoculation of an agar slant -Tube mouth is flamed, recapped, and tube is incubated - Tube of sterile agar is uncapped and mouth is flamed - Loop is flamed red hot and returned to receptacle - Inoculating loop is heated until red hot - Mouth of tube is flamed, but the loop is not heated - Cap is removed from slant culture and mouth is flamed - Organisms are removed from slant culture with loop - Slant is streaked in serpentine manner - Cap is replaced on slant culture

1. Inoculating loop is heated until red hot 2. Cap is removed from slant culture and mouth is flamed 3. Organisms are removed from slant culture with loop 4. Mouth of tube is flamed, but the loop is not heated 5. Cap is replaced on slant culture 6. Tube of sterile agar is uncapped and mouth is flamed 7. Slant is streaked in serpentine manner 8. Tube mouth is flamed, recapped, and tube is incubated 9. Loop is flamed red hot and returned to receptacle

Place in order the steps required to inoculate an agar plate from a broth culture. Flame mouth of tube, replace cap and place broth tube in test tube rack. Obtain inoculate with loop Remove cap from broth tube and flame mouth of tube Remove loop from plate, replace lid and flame loop Open plate lid and gently run loop across agar Flame inoculation loop

4 Flame mouth of tube, replace cap and place broth tube in test tube rack. 3 Obtain inoculate with loop 2 Remove cap from broth tube and flame mouth of tube 6 Remove loop from plate, replace lid and flame loop 5 Open plate lid and gently run loop across agar 1 Flame inoculation loop

When completing a quadrant streak, when do you flame the loop? A. Before you pickup a loopful of organisms from the original culture B. Before you streak quadrant one C. Before you streak quadrant two and three D. Before you return the loop to the receptacle

A. Before you pickup a loopful of organisms from the original culture C. Before you streak quadrant two and three D. Before you return the loop to the receptacle

Please select all of the statements which are TRUE regarding isolated colonies. A. The colony results from a single cell or a cluster of cells multiplying into a visible mass. B. The cells within the colony are all the same species. C. Isolated colonies form on solid nutrient media. D. Isolated colonies form in liquid nutrient media. E. Isolated colonies can only be obtained via the streak plate method.

A. The colony results from a single cell or a cluster of cells multiplying into a visible mass. B. The cells within the colony are all the same species. C. Isolated colonies form on solid nutrient media.

How is air contamination prevented when an inoculating loop is used to introduce or remove organisms from an agar plate? A. The plate lid is kept closed or lifted only slightly when inoculating. B. The plate lid is flamed before incubation. C. The loop is flamed just before introduction of organisms onto the plate surface. D. The plate lid is flamed before insertion of the loop.

A. The plate lid is kept closed or lifted only slightly when inoculating.

During streak plate technique: What is the goal of this technique? A. To promote growth of bacteria into single colonies on solid media B. To allow bacteria to grow into confluent growth on a petri dish C. To identify bacterial species based on the quadrant in which colonies form D. to kill bacteria and avoid contamination

A. To promote growth of bacteria into single colonies on solid media

During streak plate technique: How can you tell that you obtained a successful result? A. you will see single colonies after incubation B. you will see colonies touching each other in any of the quadrants C. you will see abundant growth in first quadrant D. you will see confluent growth in the last quadrant

A. you will see single colonies after incubation

Which term refers to the introduction of unwanted microorganisms? A.sterilization B. ubiquitous C. contamination D. inoculation

contamination

A substance used to support the growth of microbial life is referred to as a ___________________________. A. water B. culture medium C. inoculating loop D. incubator

culture medium

You begin your shift at the inner city outreach clinic when a young woman enters crying. She is 19 years old and 28 weeks pregnant with her second child. Her complaint is that she woke this morning leaking clear to milky-colored fluid vaginally. Her first child was born 6 weeks early due to premature rupture of membranes and she is worried this is happening again. You reassure the patient, and explain that a vaginal speculum exam will be performed and specimens will be taken for examination. Once obtaining the proper specimens, you label them appropriately, and send the wet mount and culturette to the laboratory for processing. The patient asks how microbes from her body can be grown in the lab. You explain that specimens are introduced to nutrient medium and that any growth of the microbe that appears after incubating the specimen is called the A. culture. B. colony. C. microorganism. D. infectious agent.

culture.

An agar plate that has colonies demonstrating multiple different morphologies is most likely a pure culture. True False

false

Petri dishes should be incubated with the lid side up. True False

false

true or false: Treating the work area with disinfectant will kill all forms of microbes.

false

true or false: Aseptic technique is only used when working with pure cultures since isolation has already been achieved.

false

true or false: Observation of a broth after transfer can show whether or not contamination has occurred.

false

true or false: The isolation streak plate technique is used to transfer a pure culture of bacteria from one medium to another.

false

Does this image represent a pure culture? Picture yes No

no

Ms. Hungh, a Burmese immigrant, enters your clinic with her interpreter complaining of fatigue, weight loss, persistent cough, and rust-colored sputum. The interpreter explains that Ms. Hungh has had this cough for many months in her home country and, now that she is in America, is seeking assistance for her condition. Culturing of the sputum resulted in the growth of distinct colonies on the medium, and the technician informs you that further isolation by subculturing is now needed. You understand that this is accomplished by taking a bit of growth from an isolated colony and inoculating a separate medium, resulting in the production of a A. broth. B. pure culture. C. mixed culture. D. diagnosis.

pure culture.

To retrieve a sample from a culture tube with an inoculating loop, the cap of the tube is removed and held with the fingers of the loop hand removed with the fingers of the loop hand and placed in the fingers of the tube hand removed with the fingers of the loop hand and placed on the laboratory bench any of these methods can be used removed and held in one's teeth

removed and held with the fingers of the loop hand

Which of the following is essential for development of discrete, isolated colonies? A. solid medium B. broth medium C. selective medium D. assay medium E. differential medium

solid medium

What type of isolation technique is most effective for the majority of applications? A. loop dilution B. spread plate C. streak plate D. culture plate E. pour plate

streak plate

true or false: Once focus is achieved at one magnification, a higher-power objective lens can be rotated into position without fear of striking the slide.

true

true or false: Patients' samples need to be cultured and isolate single colonies, before carrying out other specific testing to identify microbe.

true

Microorganisms are found in nearly every environment and are said to be ____________________. A. omnipresent B. ubiquitous C. contaminated D. omniscient

ubiquitous

From the following list, select the scenarios for which using the isolation streak plate method would be used. A. Determining if a newly discovered bacteria is capable of growing in an enriched media. B. Determining the bacteria from a patient nasal swab may be causing an infection. C. Determining the diversity of bacteria in a water sample. D. Detecting the presence of a specific bacteria in a swab sample from a patient wound. E. Detecting the ability of an organism to breakdown specific sugars for energy production.

B. Determining the bacteria from a patient nasal swab may be causing an infection. C. Determining the diversity of bacteria in a water sample. D. Detecting the presence of a specific bacteria in a swab sample from a patient wound.

Put these lenses in the order they should be used when viewing a specimen with a brightfield microscope. A. Oil immersion lens B. Low power lens C. High dry lens

B. Low power C. High dry A. Oil immersion

Which of the steps described here prevents the contamination of just-washed hands? A. Lathering under running water for 30 seconds. B. Use of a paper towel to turn off a faucet. C. Wetting the hands before applying soap. D. Use of hot water.

B. Use of a paper towel to turn off a faucet.

When a hypothesis has been thoroughly supported by long-term study and data, it is considered. A. proved. B. a theory. C. a law. D. a speculation.

B. a theory

Which is the most appropriate disposal for a swab used to culture your throat during lab? A. sharps container B. biohazard bag C. recycle bin D. normal trash receptacle

B. biohazard bag

How is an inoculating loop or needle sterilized prior to use? A. soaking in 95% ethyl alcohol for several minutes and allowing to air dry B. holding in the hottest part of the bunsen burner flame until red-hot C. exposure to UV radiation for 15 seconds D. dipping into a beaker of boiling water

B. holding in the hottest part of the Bunsen burner flame until red-hot

Where should the label be placed on Petri dishes? A. on the edge B. on the bottom C. whichever location is most convenient D. on the lid

B. on the bottom

When an inoculated plate (in particular streak plate) is placed inside incubator, it is important to invert plate because:_________ (invert means with agar side up) A. this avoids contamination of the colonies from external contaminants B. this position of the plate avoids condensation from dripping into the colonies C. this position of the plate promotes more growth D. this position of the plate promotes faster growth

B. this position of the plate avoids condensation from dripping into the colonies

What biosafety levels do most introductory microbiology students work with? A. All of the above levels B. BSL-2 C. BSL-3 D. BSL-1

BSL-1

During streak plate technique: Why is it necessary to flame the loop in between streaks? A. To grab bacteria from previous streaked area going into new streaked area B. In order to count colonies from streaked areas based on previous areas C. To reduce number of bacteria from previous streaked area going into new streaked area D. to kill bacteria and avoid contamination

C. To reduce number of bacteria from previous streaked area going into new streaked area

You begin your shift at the inner city outreach clinic when a young woman enters crying. She is 19 years old and 28 weeks pregnant with her second child. Her complaint is that she woke this morning leaking clear to milky-colored fluid vaginally. Her first child was born 6 weeks early due to premature rupture of membranes and she is worried this is happening again. You reassure the patient, and explain that a vaginal speculum exam will be performed and specimens will be taken for examination. Once obtaining the proper specimens, you label them appropriately, and send the wet mount and culturette to the laboratory for processing. No ferning of the vaginal fluid is detected, and you reassure your patient that her membranes have not ruptured. You explain that the laboratory technicians will use which five basic techniques (in order) to manipulate, grow, examine, and characterize any microorganisms present in the collected specimens? A. isolation, incubation, inspection, identification, and inoculation B. identification, isolation, incubation, inspection, and inoculation C. inoculation, incubation, isolation, inspection, and identification D. inspection, identification, isolation, incubation, and inoculation

C. inoculation, incubation, isolation, inspection, and identification

An inoculating loop or needle is sterilized in a flame or incinerator: A. until the handle is bright red for exactly 5 minutes B. until the tip is bright red C. until the entire wire is bright red by one brief passage

C. until the entire wire is bright red

To cultivate (verb) or observable growth (noun) Inoculation Medium Culture

Culture

Place in order the steps necessary to carry out proper isolation streak plate technique. A. Flame loop and obtain inoculum from mixed culture B. Flame loop, drag across side of first inoculation on the agar plate and streak one third of the plate C. Place loop in inoculated third of plate and streak the final third portion of the plate D. Inoculate one half of an agar plate E. Flame loop and replace plate lid

Flame loop and obtain inoculum from mixed culture Inoculate one half of an agar plate Place loop in inoculated third of plate and streak the final third portion of the plate Flame loop, drag across side of first inoculation on the agar plate and streak one third of the plate Flame loop and replace lid

Process of adding a microbe to a growth material inoculation Medium Culture

Inoculation

The material which provides the nutrients for growth inoculation Medium Culture

Medium

You have cultured several different objects and surfaces in the environment. Which of the following indicates a more abundant bacterial population? The item with the greatest number of microbial colonies on an agar plate The item with the largest colonies on an agar plate

The item with the greatest number of microbial colonies on an agar plate

Media in slants and Petri dishes is solidified by the addition of __________________. A. gelatin B. polyacrylamide C. polypeptides D. agar

agar

What type of medium provides the best opportunity for growing and observing the morphology of isolated colonies? broth agar plates slants

agar plates