Protein: Test procedures
What is the purpose of the BUN test?
Determine the amount of Urea in the blood
Which of the following is the principle for the biuret method for total protein analysis?
Formation of vilet colored chelate between Cu2+ ions and peptide bonds
Which of the following methods may be employed to definitively identify Bence Jones proteins?
Immunoelectrophoresis
Which of the following methods may be employed to definitively identify Bence-Jones proteins?
Immunoelectrophoresis
Which of the following test confirms the presence of Bence Jones proteinsuria?
Immunoelectrophoresis
The migration rate of proteins on cellulose acetate is primarily the results of
Ionic charge on the proteins
Which of the following analytes will be falsely increased due to hemolysis?
K Mg Fe TP
Which of the following conditions is most likely when an oligoclonal band is seen in CSF electrophoresis withhout a corresponding serum peak?
Multiple sclerosis
Protein in a buffer that sets the pH at 8.6 will become_______ charged and migrate to the _________
Negative, anode
The property MOST responsible for the migration of protein in an electrical field is:
Net surface charge
There are several different types of media that can be used in electrophoresisi. Most methods today use gel. cellulose acetate, agarose, or polyacyrlamide gel. Which statement is true?
Polyacry;amide gel have the greatest resolution or separtion of solutes
There are several different types of media that can be used in electrophoresis. Most methods today use a gel- cellulose acetate, agarose, or polyacrylamide gel. Which one of the following statements is true regarding these gels?
Polyacrylamide gels have the greatest resolution or separation of solutes
Which of the following analytes will be falsely elevated due to hemolysis?
Potassium, Magnesium, Iron, Total protein
Which of the following contributes to specific gravity but does NOT contribute to osmolality?
Protein
In serum protein electrophoresis all of the following proteins reside in the band closets to the anodal EXCEPT
RBP Transthyetin Albumin are all correct
Which of the following methods would be used to confirm the presence of Bence Jones protein in the Urine:
Urine protein electrophoresisi or immunoelectrophoresis
What causes polyacrylamide gels to have greater resolution of proteins than agrose gels?
Varying pore size in each polyacrylamide layer
What causes polyacrylamide gels to have great resolution of proteins than agrase gels?
Varying pore size in each polyacylamide layer
Left is normal and right is from a patient with a particular disorder gamma is bridge
X-linked agammaglobulinemia
Which of the following proteins has the FASTEST electrophoretic mobility?
aLBUMIN
A routine serum protein electrophoresisi with normal proteins results in _______ zones of bands
five
The biuret reaction for the analysis of serum protein depends on the number of:
peptide bonds
Which statement is correct for a protein with an isoelectric point (PI) of 7.0?
At pH <7.0, this protein will be positively charged
Using the formula Creatine clearance: Urine Creatinine- 100 Plasma creatinine- 1.9 Urine Vol- 1375 Surface area- 1.2
72mL
Which of the following GFR values is considered to be in renal failure?
14 mL/min
A 56 year old women Plasma creatinine- 2.4 Urine creatine- 65 Urine volume- 1400
26.32
How many distinct bands are seperated during a standard serum protein electrophoresisi?
5
Serum proteins can be separated by cellulose acetate electrophoresisi into how many basic fractions?
5 fractions
Given the following information, calculate the results in mg/24 hrs for a 24 hour urine protein. Total volume for 24 hours =2400mL Urine protein=2.7 mg/dL
64.8 mg/24 hrs
Which of the following proteins has the fastest electrophoretic mobility?
Albumin
Which protein is represcented by the band labeled by the number 4 on the SPE scan?
Albumin
The lab test used to determine the presence of neural tube defect is
Alpha fetoprotein
Which of the following tests would be employed in order to detect neural tube defects?
Alpha fetoprotein
All of the following have an impact on electrophoresisi techniques in the clinical lab EXCEPT?
Amount of light present in electrophoresis box
All of the following are quantitiatative methods for the determination of albuminw except?
Sulfosalicyclic acid precipitation
What test can be used as a confirmatory test for proteinuria?
Sulfosalicylic acid test
Which substance is used in the Jendrassik-Grof method to accelarate the reaction of unconjugated bilirubin with the diazo reagent?
Caffine benzoate
Which dye-binding method uses an increase of absorbance at 595nm to determine the total protein concentration of the specimen
Coomassie brilliant blue 250
Which of the following tests would be used in the assessment of glomerular filtration?
Creation clearance
Select the primary reagent components used in the jaffe reaction for creatinine
Saturated picric acid and sodium hydroxide
The following results were obtained on an SPE scan. what can you infer about the specimen from these results?
There is an interfering substance present in the specimen such as hemoglobin due to hemolysis
The pI of a protein is 9.2. This protein is placed in an electrical field where a buffer sets the pH at 10.0. Select the correct statement regarding the electrophoretic migration of this protein.
This protein will have a net negative charge and will migrate toward the anode, the positively charged electrode.
TIBC is an indirect measurement of which of the following:
Transferrin
Which of the following is currently considered to be a good test for screening persons for hereditary hemchromatosis due to its sensitivity and specificity for iron overload?
Transferrin saturation
Which of the following is currently considered to be a good test for screening persons for hereditary hemochromatosis (HH) due to its sensitivity and specificity for iron overload?
Transferrin saturation
All of the following specimen processing examples are considered processing errors that can have a negative impact on the test results EXCEPT?
Transporting an ammonia specimen to the laboratory in an ice slurry
