Protein: Test procedures

What is the purpose of the BUN test?

Determine the amount of Urea in the blood

Which of the following is the principle for the biuret method for total protein analysis?

Formation of vilet colored chelate between Cu2+ ions and peptide bonds

Which of the following methods may be employed to definitively identify Bence Jones proteins?

Immunoelectrophoresis

Which of the following methods may be employed to definitively identify Bence-Jones proteins?

Immunoelectrophoresis

Which of the following test confirms the presence of Bence Jones proteinsuria?

Immunoelectrophoresis

The migration rate of proteins on cellulose acetate is primarily the results of

Ionic charge on the proteins

Which of the following analytes will be falsely increased due to hemolysis?

K Mg Fe TP

Which of the following conditions is most likely when an oligoclonal band is seen in CSF electrophoresis withhout a corresponding serum peak?

Multiple sclerosis

Protein in a buffer that sets the pH at 8.6 will become_______ charged and migrate to the _________

Negative, anode

The property MOST responsible for the migration of protein in an electrical field is:

Net surface charge

There are several different types of media that can be used in electrophoresisi. Most methods today use gel. cellulose acetate, agarose, or polyacyrlamide gel. Which statement is true?

Polyacry;amide gel have the greatest resolution or separtion of solutes

There are several different types of media that can be used in electrophoresis. Most methods today use a gel- cellulose acetate, agarose, or polyacrylamide gel. Which one of the following statements is true regarding these gels?

Polyacrylamide gels have the greatest resolution or separation of solutes

Which of the following analytes will be falsely elevated due to hemolysis?

Potassium, Magnesium, Iron, Total protein

Which of the following contributes to specific gravity but does NOT contribute to osmolality?

Protein

In serum protein electrophoresis all of the following proteins reside in the band closets to the anodal EXCEPT

RBP Transthyetin Albumin are all correct

Which of the following methods would be used to confirm the presence of Bence Jones protein in the Urine:

Urine protein electrophoresisi or immunoelectrophoresis

What causes polyacrylamide gels to have greater resolution of proteins than agrose gels?

Varying pore size in each polyacrylamide layer

What causes polyacrylamide gels to have great resolution of proteins than agrase gels?

Varying pore size in each polyacylamide layer

Left is normal and right is from a patient with a particular disorder gamma is bridge

X-linked agammaglobulinemia

Which of the following proteins has the FASTEST electrophoretic mobility?

aLBUMIN

A routine serum protein electrophoresisi with normal proteins results in _______ zones of bands

five

The biuret reaction for the analysis of serum protein depends on the number of:

peptide bonds

Which statement is correct for a protein with an isoelectric point (PI) of 7.0?

At pH <7.0, this protein will be positively charged

Using the formula Creatine clearance: Urine Creatinine- 100 Plasma creatinine- 1.9 Urine Vol- 1375 Surface area- 1.2

72mL

Which of the following GFR values is considered to be in renal failure?

14 mL/min

A 56 year old women Plasma creatinine- 2.4 Urine creatine- 65 Urine volume- 1400

26.32

How many distinct bands are seperated during a standard serum protein electrophoresisi?

5

Serum proteins can be separated by cellulose acetate electrophoresisi into how many basic fractions?

5 fractions

Given the following information, calculate the results in mg/24 hrs for a 24 hour urine protein. Total volume for 24 hours =2400mL Urine protein=2.7 mg/dL

64.8 mg/24 hrs

Which of the following proteins has the fastest electrophoretic mobility?

Albumin

Which protein is represcented by the band labeled by the number 4 on the SPE scan?

Albumin

The lab test used to determine the presence of neural tube defect is

Alpha fetoprotein

Which of the following tests would be employed in order to detect neural tube defects?

Alpha fetoprotein

All of the following have an impact on electrophoresisi techniques in the clinical lab EXCEPT?

Amount of light present in electrophoresis box

All of the following are quantitiatative methods for the determination of albuminw except?

Sulfosalicyclic acid precipitation

What test can be used as a confirmatory test for proteinuria?

Sulfosalicylic acid test

Which substance is used in the Jendrassik-Grof method to accelarate the reaction of unconjugated bilirubin with the diazo reagent?

Caffine benzoate

Which dye-binding method uses an increase of absorbance at 595nm to determine the total protein concentration of the specimen

Coomassie brilliant blue 250

Which of the following tests would be used in the assessment of glomerular filtration?

Creation clearance

Select the primary reagent components used in the jaffe reaction for creatinine

Saturated picric acid and sodium hydroxide

The following results were obtained on an SPE scan. what can you infer about the specimen from these results?

There is an interfering substance present in the specimen such as hemoglobin due to hemolysis

The pI of a protein is 9.2. This protein is placed in an electrical field where a buffer sets the pH at 10.0. Select the correct statement regarding the electrophoretic migration of this protein.

This protein will have a net negative charge and will migrate toward the anode, the positively charged electrode.

TIBC is an indirect measurement of which of the following:

Transferrin

Which of the following is currently considered to be a good test for screening persons for hereditary hemchromatosis due to its sensitivity and specificity for iron overload?

Transferrin saturation

Which of the following is currently considered to be a good test for screening persons for hereditary hemochromatosis (HH) due to its sensitivity and specificity for iron overload?

Transferrin saturation

All of the following specimen processing examples are considered processing errors that can have a negative impact on the test results EXCEPT?

Transporting an ammonia specimen to the laboratory in an ice slurry