9.2 Gene Transfer, Genetic Engineering, and Genomics: Genet Engineering

Synonyms for restriction endonucleases

-restriction enzyme -restriction nuclease -endonuclease

Name two things bacteria can be genetically engineered to do

1. break down toxic wastes 2. produce antibiotics

Name two things plants have been engineered using microbial genes for

1. herbicidal activity 2. viral resistance

Where does EcoRI cut?

At the specific palindrome sequence

the commercial and industrial products derived from genetic engineering

Biotechnology

A recombinant DNA molecule contains what

DNA fragments spliced together from 2 or more organisms

In restriction endonuclease, the fragments are joined by

DNA ligase

Genetic Engineering Was Born from what

Genetic Recombination

On a plasmid, a restriction site is also called

MCS or multiple cloning site

Examples of restriction endonuclease

Pst I, EcoRI, HindIII, BamHI, Sall, each cut at a unique palindrome DNA sequence

Why does the DNA need to be single stranded?

So it can hybridize of course!

Cows produce more milk when injected with _____ growth hormone produced by engineered bacteria

bovine

involves changing the genetic material in an organism to alter its traits or products

genetic engineering

•Specific nucleotide sequences in pathogens allow us to what

identify them using DNA probes

Recombinant vaccines can be safer than traditional vaccines using what

killed or attenuated microbes

Specific stretches of nucleotides can be cut with specific what

restriction endonucleases

True or false: the -genes responsible for producing human insulin can be cloned into bacteria

true