9.2 Gene Transfer, Genetic Engineering, and Genomics: Genet Engineering
Synonyms for restriction endonucleases
-restriction enzyme -restriction nuclease -endonuclease
Name two things bacteria can be genetically engineered to do
1. break down toxic wastes 2. produce antibiotics
Name two things plants have been engineered using microbial genes for
1. herbicidal activity 2. viral resistance
Where does EcoRI cut?
At the specific palindrome sequence
the commercial and industrial products derived from genetic engineering
Biotechnology
A recombinant DNA molecule contains what
DNA fragments spliced together from 2 or more organisms
In restriction endonuclease, the fragments are joined by
DNA ligase
Genetic Engineering Was Born from what
Genetic Recombination
On a plasmid, a restriction site is also called
MCS or multiple cloning site
Examples of restriction endonuclease
Pst I, EcoRI, HindIII, BamHI, Sall, each cut at a unique palindrome DNA sequence
Why does the DNA need to be single stranded?
So it can hybridize of course!
Cows produce more milk when injected with _____ growth hormone produced by engineered bacteria
bovine
involves changing the genetic material in an organism to alter its traits or products
genetic engineering
•Specific nucleotide sequences in pathogens allow us to what
identify them using DNA probes
Recombinant vaccines can be safer than traditional vaccines using what
killed or attenuated microbes
Specific stretches of nucleotides can be cut with specific what
restriction endonucleases
True or false: the -genes responsible for producing human insulin can be cloned into bacteria
true
