Chapter 12: Biotechnology

Which of the following are applications of recombinant DNA technology

-making crops that are pest resistant -making proteins are useful as medicines -Making bacteria that can clean up toxic waste

In gel electrophoresis DNA molecules migrate from

Negative to positive end of the gel

This video explaining the CRISPR-Cas9 system.what is Cas9?

A protein that cuts DNA at specific sequences

In a PCR reaction, the strands of DNA are first separated by

heating

Bacteria act as microscopic protein factories

True

Bacteria are the source of plasmids. True or false

True

If I cut a piece of DNA into one long and two short pieces, which of the following shells shows how it will look

This gel has one brand near the top, which represents a long piece of DNA and two bands near the bottom, which represents too short pieces of DNA.

When is PCR particularly applicable?

When there are small quantities of DNA to analyze

What is the correct sequence of events that occur in a PCR reaction?

separation of DNA strands; addition of primers; use of DNA polymerase to produce second strand of DNA

The unpaired nucleotides produced by the action of restriction enzymes are referred to as

sticky ends

How would CRISPR-Cas9 system have to be costomized for this application?

-The guide aren't they would have to target the section of the HB with mutation -Bone marrow cells would have to be targeted for modifications -a normal copy of the HBb Gene in this model represents a DNA nucleotide, which part of a DNA nucleotide contains a negative charge and would have to be provided

The sticky end of the DNA restriction fragment shown here will pair with a DNA restriction fragment with a sticky end

ACGT

I've accidentally hook up the wires to this gel, electrophoresis chamber backwards with a positive pole the top and the negative pull at the bottom. If I turn on the power with it hooked up this way what will happen to the DNA ?

DNA normally moves down through a gel due to the attraction of opposite charges. In this case the DNA would move up the gel towards the positive pull at the top.

The bacterial chromosome is inserted into human cells

False

The bacterial chromosome provides genetic information for making the protein of interest

False

Which of these genes codes for protein that plays a role in growth?

GH1

Which of these genes codes for protein that plays a role in white blood cell function

MPO

Used to create a large sample of DNA for testing from a small sample of DNA. What is that method called?

PCR machine

Which part of the DNA nucleotide contains a negative charge

Phosphate

DNA polymerase is a heat sensitive enzyme. What is one thing that would need to be considered concerning the activity of the enzyme in PCR when the temperature is heated during each cycle to separate the DNA strands.

The DNA polymers could be denatured.

The RP13 gene of chromosome 17 codes for a protein

involved in eye development

The TP53 gene of chromosome 17 codes for a protein

involved in the regulation of the cell cycle

The gene that codes for gastrin is located on the _______ of chromosome 17.

q arm