Chapter 9 Review

Handles microbes such as Escherichia coli. Minimal precautions include hand washing with antibacterial soap and disinfecting surfaces. Which biosafety level is this considered? (a.) BSL 1 (b) BSL 2 (c.) BSL 3 (d.) BSL4

(a.) BSL 1

The endospores of which organism are used as a biological indicator of sterilization? (a.) Bacillus stearothermophilus (b.) Salmonella enterica (c.) Mycobacterium tuberculosis (d.) Staphylococcus aureus

(a.) Bacillus stearothermophilus

Biosafety level 3 includes ___________ (a.) double sets of entry doors (b.) pressurized suits (c.) showers in entryways (d.) all of the above

(a.) double sets of entry doors

Which of the following types of radiation is more widely used as an antimicrobial technique? (a.) electron beams (b.) visible light waves (c.) radio waves (d.) microwaves

(a.) electron beams

In practical terms in everyday use, which of the following statements provides the definition of sterilization? (a.) eliminates organisms and their spores or endospores (b.) eliminates harmful microorganisms and viruses (c.) eliminates prions (d.) eliminates hyperthermophiles

(a.) eliminates organisms and their spores or endospores

Researchers add a suspension of a bacterium to a suitable concentration of the chemical being tested. They are then moved to a growth medium containing a disinfectant deactivator and then incubated. Which of the following describes this procedure? (a.) kelsey sykes capacity test (b.) use dilution test (c.) filter paper test (d.) in use test

(a.) kelsey sykes capacity test

Which of the following disinfectants acts against cell membranes? (a.) phenol (b.) peracetic acid (c.) silver nitrate (d.) glutaraldehyde

(a.) phenol

Which class of surfactant is most soluble in water? (a.) quaternary ammonium compounds (b.) alcohols (c.) soaps (d.) peracetic acids

(a.) quaternary ammonium compounds

The decimal reduction time for endospores of Bacillus cereus in milk is 36.2 minutes at 95°C. How long should it take to reduce a population of 1015 endospores to 1 endospore? (a) 36 minutes (b) 9 hours 5 minutes (c) 2 hours 5 minutes (d) 10 hours 5 minutes

(b) 9 hours 5 minutes

Facilities are designed for handling moderately hazardous agents. Extreme precautions are taken with contaminated sharp objects, procedures that might produce aerosols are conducted within safety cabinets. What biosafety level is this? (a.) BSL 1 (b) BSL 2 (c.) BSL 3 (d.) BSL4

(b.) BSL 2

which of the following substances or processes kills microorganisms on laboratory surfaces? (a.) antiseptics (b.) disinfectants (c.) degermers (d.) pasteurization

(b.) disinfectants

Which of the following chemicals is active against bacterial endospores? (a.) copper ions (b.) ethylene oxide (c.) ethanol (d.) triclosan

(b.) ethylene oxide

Measures the efficacy of disinfectants and antiseptics against specific microbes. (a.) kelsey sykes capacity test (b.) use dilution test (c.) filter paper test (d.) in use test

(b.) use dilution test

Which of the following tests is the U.S. standard for determining the activity of a disinfectant in a particular environment or application? (a.) Phenol Coefficient (b.) use-dilution test (c.) The Kelsey-Sykes capacity test (d.) in-use test

(b.) use-dilution test

A Surgical field is __________ (a) Sanitized (b) Disinfected (c) Aseptic (d) Sterile

(c) Aseptic

Which of the following would contribute to the decreased efficiency of an antimicrobial agent? (a) Higher temperatures (b) Longer exposer to cells (c) Presence of vomit (d) Higher concentrations of the agent

(c) Presence of vomit

The use of steam heat under pressure to sterilize chemicals and objects that can tolerate moist heat is called... (a.) Desiccation (b.) Pasteurization (c.) Autoclave (d.) Boiling

(c.) Autoclave

All safety cabinets are required to contain HEPA filters and include entry through double sets of doors and lower air pressure so that no air leaves the room. What biosafety level would this be considered as? (a.) BSL 1 (b) BSL 2 (c.) BSL 3 (d.) BSL4

(c.) BSL 3

a sample of E coli has been subjected to heat for a specific time, and 90% of the cells have been destroyed. Which of the following terms best describes this event? (a.) thermal death point (b.) thermal death time (c.) decimal reduction time (d.) none of the above

(c.) decimal reduction time

The preservation of beef jerky from microbial growth relies on with method of microbial control? (a.)filtration (b.) lyophilization (c.) desiccation (d.) radiation

(c.) desiccation

Which of the following substances is least toxic to humans? (a.) carbolic acid (b.) glutaraldehyde (c.) hydrogen peroxide (d.) formalin

(c.) hydrogen peroxide

A researcher dips several metal cylinders into broth cultures of bacteria and briefly dries them at 37'C. Which of the following describes this procedure? (a.) disk diffusion test (b.) filter paper test (c.) use dilution test (d.) in use test

(c.) use dilution test

Which of the following microbial control procedures disrupts hydrogen bonding in DNA? (a) Noninzing radiation (b) Heavy metals (c) Autoclaving (d) Ionizing radiation

(d) Ionizing radiation

Which of the following is not a factor affecting efficacy of an antimicrobial method? (a) Environmental conditions such as temperature and pH (b) Relative susceptibility of microbes, killing the hardiest microbes first (c) Nature of site to be treated (d) The biosafety level of the lab to be cleaned

(d) The biosafety level of the lab to be cleaned

All of the following microbial control methods are bactericidal except.... (a) autoclaving (b) incineration (c) ionizing radiation (d) lyophilization

(d) lyophilization

All of the following antimicrobial agents function by disrupting cell membranes except __________. (a) detergent (b) phenol (c) Isopropanol (d) mercury

(d) mercury

Entry is strictly controlled through electronically sealed airlocks with multiple showers, a vacuum, a UV light room and other precautions to destroy all traces of biohazard. This is what biosafety level? (a.) BSL 1 (b) BSL 2 (c.) BSL 3 (d.) BSL4

(d.) BSL 4

Which of the following disinfectants contains alcohol? (a.) an iodophor (b.) a quad (c.) formalin (d.) a tincture of bromine

(d.) a tincture of bromine

the microbial death rate is used to measure _____________________. (a.) the efficiency of a detergent (b.) the efficiency of an antiseptic (c.) the efficiency of sanitization techniques (d.) all of the above

(d.) all of the above

which of the following statements are true concerning the selection of an antimicrobial agent? (a.) an ideal antimicrobial agent is stable during storage (b.) an ideal antimicrobial agent is fast acting (c.) ideal microbial agents do not exist (d.) all of the above

(d.) all of the above

Which of the following adjectives best describes a surgical procedure that is free of microbial contaminants? (a.) disinfected (b.) sanitized (c.) degermed (d.) aseptic

(d.) aseptic

Which antimicrobial chemical has been used to sterilize spacecraft? (a.) phenol (b.) alcohol (c.) heavy metal (d.) ethylene oxide

(d.) ethylene oxide

Which of the following substances would most effectively inhibit anaerobes? (a.) phenol (b.) silver (c.) ethanol (d.) hydrogen peroxide

(d.) hydrogen peroxide

A company that manufactures an antimicrobial cleaner for kitchen counters claims that its product is effective when used in a 50% water solution. By what means might scientists best verify this statement? (a.) disk diffusion test (b.) phenol coefficient (c.) filter paper test (d.) in use test

(d.) in use test

Which of the following items functions most like an autoclave? (a.) boiling pan (b.) incinerator (c.) microwave oven (d.) pressure cooker

(d.) pressure cooker

which of the following terms best describes the disinfection of cafeteria plates? (a.) pasteurization (b.) antisepsis (c.) sterilization (d.) sanitization

(d.) sanitization