Lab #2: Protein modeling and quantification of protein content in lima beans

What is the biuret test for and how does it work?

The Biuret reagent tests for peptide bonds by reacting with the peptide bonds in proteins to form purple color. The darker the color, the more bonds are present.

Spectrophotometer video

https://www.dropbox.com/s/wte6gs0et9pudcg/How%20to%20use%20a%20Spectro-photometer.mp4

How does a spectrophotometer work?

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What is the purpose of centrifugation?

Seperate substances of greater or lesser density

How are proteins composed and how do they fold?

Proteins are made of amino acids, and it is the order of the amino acids in a polypeptide chain that determines how it folds in 3D space.

What cellular process creates proteins?

RNA Translation via ribosomes

What does RPM stand for?

Revolutions per minute

What determines if something is found in the pellet or supernatant?

density??