Lab exam
Which objective lens requires oil to be applied?
100x
What lens could get dirty with oil if you move the revolving nosepiece in the wrong direction after viewing under oil immersion?
40x
You have successfully focused on an object with the 10x and 40x objective lenses. You have rotated the 100x objective lens into position but are unable to obtain a sharp image by rotating the fine focus adjustment knob. Which of the following is most likely to correct this problem?
Add a drop of immersion oil before rotating the 100x lens into position.
Arrange the following steps of applying immersion oil in the correct sequence
1. Focus on the specimen using the 40x objective lens. 2. Turn the objectives to half way between the 40x lens and the 100x lens. 3. Apply a drop of immersion oil directly onto area of the slide you were viewing. 4. Rotate the 100x lens over the microscope slide, and use the fine focus adjustment knob to sharpen the image.
Indicate the correct order of steps as you bring an object into focus under high power.
1. Place slide on stage and secure with stage clips. 2. Turn on the light and center the specimen over the light source. 3. Focus specimen on scanning objective using coarse focus adjustment 4. Rotate to low power objective, then use fine focus to sharpen the image 5. Rotate high power lens into position. 6. Readjust fine focus under high power to produce the sharpest image
The total magnification achieved using a 10× objective lens with a 10× eyepiece lens is 20×.
False
The __________ causes the stage to move upward or downward.
coarse adjustment knob
After focusing on an object with the low power objective lens, you are trying to bring it into focus under the 40x objective lens, but the field of view is very dark. Which of the following may remedy this problem?
increase the light intensity
A change in what component of microscopy influences the apparent size of the objects being viewed?
magnification
Which objective lens provides the highest total magnification?
oil immersion
Using oil immersion is useful because
oil immersion increases the resolving power of the specimen.
